FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3088334671> ?p ?o ?g. }

• W3088334671 endingPage "114389" @default.
• W3088334671 startingPage "114389" @default.
• W3088334671 abstract "Abstract Here, an amphiphilic polymer, oleanolic acid conjugated methoxy-poly (ethylene glycol)-poly (D, l -lactide) (mPEG-PLA-OA) was synthesized, which self-assembled in aqueous environment and loaded the poorly soluble anticancer agent, doxorubicin. Various physico-chemical characterization studies on polymer and DOX-loaded polymeric micelles (DOX-mPEG-PLA-OA) at various drug to polymer (1:5/10/15) (DTP) ratios were performed, which included characterizations via UV, IR, NMR spectroscopy and gel permeation chromatography for polymers, and dynamic light scattering, DSC, XRD techniques, determination of critical micelles concentration, percent drug loading, encapsulation efficiency, drug release, kinetic stability and haemolysis studies for micelles. Further, DOX-mPEG-PLA-OA were evaluated for their cellular uptake, cytotoxicity, ability to induce apoptosis, DNA fragmentation, nuclear damage, ROS generation, and mitochondrial membrane depolarization in oral squamous cell carcinoma (FaDu HTB-43) and murine melanoma (B16F10) in monolayer and in FaDu HTB-43 3D cell culture systems. DOX-mPEG-PLA-OA at DTP ratios. 1:5/10/15 showed physico-chemical parameters in the following range: particle sizes. ~210.3–225.1 nm, DOX encapsulation efficiency. 29–35% and DOX-loading. 2.2–5%. DOX-mPEG-PLA-OA micelles (1:15) were stable as proven by kinetic stability study, and showed sustained release of encapsulated DOX for over 60 h. The cell viability study revealed that DOX-mPEG-PLA-OA micelles (1:15) demonstrated highest time- and dose dependent cytotoxicity compared to free DOX and micelles of other DTP ratios. Following treatment, the DOX-mPEG-PLA-OA micelles (1:5/10/15) induced highest cellular apoptosis in the range of 28.17–48.1, compared to free DOX (9.5 ± 2.52), and mPEG-PLA-OA micelles (25.4 ± 2.91), DOX-mPEG-PLA-OA micelles (1:15) being the strongest apoptosis inducer (48.1 ± 2.68) as determined by flow cytometry-mediated annexin V assay. DOX-mPEG-PLA-OA micelles (1:15) induced significantly higher DNA fragmentation, nuclear condensation, ROS generation, and mitochondrial membrane permeabilization compared to free Dox in monolayer FaDu HTB-43. DOX-mPEG-PLA-OA micelles (1:15) showed reasonably higher penetration and stronger growth inhibition in FaDu HTB-43 spheroids compared to free DOX and micelles of other DTP ratios. In-vivo pharmacokinetic studies showed that the DOX-mPEG-PLA-OA micelles demonstrated enhanced blood circulation time with decreased rate of clearance than free Dox (t1/2. 33.5 ± 2.5 h vs. 1.77 ± 0.32 h, and Cmax. 59.7 ± 2.2 vs. 2.3 ± 0.1, respectively). Immunohistochemistry revealed no significant toxicity in major organs of DOX-mPEG-PLA-OA micelles treated rats. The series of studies indicated that the OA-conjugated mPEG-PLA micelles served as an efficient nanocarriers for DOX, demonstrated DOX, and OA combined superior anticancer effect, sustained DOX level in blood circulation for up to 96 h, and no sign of major organs toxicity. Therefore, this newly developed DOX, OA combined micelles displayed its potential as promising alternative to DOX-based conventional therapy, and warrants further investigation for clinical application, especially for the treatment of oral cancer." @default.
• W3088334671 created "2020-10-01" @default.
• W3088334671 creator A5011840568 @default.
• W3088334671 creator A5036638461 @default.
• W3088334671 creator A5039785987 @default.
• W3088334671 creator A5081881235 @default.
• W3088334671 creator A5089684202 @default.
• W3088334671 date "2020-12-01" @default.
• W3088334671 modified "2023-10-12" @default.
• W3088334671 title "Oleanolic acid-conjugated poly (D, l-lactide)-based micelles for effective delivery of doxorubicin and combination chemotherapy in oral cancer" @default.
• W3088334671 cites W1966322089 @default.
• W3088334671 cites W1997470573 @default.
• W3088334671 cites W2013765163 @default.
• W3088334671 cites W2024255080 @default.
• W3088334671 cites W2060868862 @default.
• W3088334671 cites W2066745755 @default.
• W3088334671 cites W2076226743 @default.
• W3088334671 cites W2084505104 @default.
• W3088334671 cites W2086374939 @default.
• W3088334671 cites W2213763060 @default.
• W3088334671 cites W2213937043 @default.
• W3088334671 cites W2241433122 @default.
• W3088334671 cites W2277087960 @default.
• W3088334671 cites W2298640980 @default.
• W3088334671 cites W2317393487 @default.
• W3088334671 cites W2330266140 @default.
• W3088334671 cites W2331140348 @default.
• W3088334671 cites W2342754223 @default.
• W3088334671 cites W2474814867 @default.
• W3088334671 cites W2558871978 @default.
• W3088334671 cites W2583238555 @default.
• W3088334671 cites W2586464879 @default.
• W3088334671 cites W2613707220 @default.
• W3088334671 cites W2750805973 @default.
• W3088334671 cites W2753308895 @default.
• W3088334671 cites W2783103717 @default.
• W3088334671 cites W2789674962 @default.
• W3088334671 cites W2793018507 @default.
• W3088334671 cites W2807997070 @default.
• W3088334671 cites W2898883774 @default.
• W3088334671 cites W2947914445 @default.
• W3088334671 cites W2951392851 @default.
• W3088334671 cites W2975591999 @default.
• W3088334671 cites W4243722521 @default.
• W3088334671 doi "https://doi.org/10.1016/j.molliq.2020.114389" @default.
• W3088334671 hasPublicationYear "2020" @default.
• W3088334671 type Work @default.
• W3088334671 sameAs 3088334671 @default.
• W3088334671 citedByCount "13" @default.
• W3088334671 countsByYear W30883346712022 @default.
• W3088334671 countsByYear W30883346712023 @default.
• W3088334671 crossrefType "journal-article" @default.
• W3088334671 hasAuthorship W3088334671A5011840568 @default.
• W3088334671 hasAuthorship W3088334671A5036638461 @default.
• W3088334671 hasAuthorship W3088334671A5039785987 @default.
• W3088334671 hasAuthorship W3088334671A5081881235 @default.
• W3088334671 hasAuthorship W3088334671A5089684202 @default.
• W3088334671 hasConcept C112613896 @default.
• W3088334671 hasConcept C11268172 @default.
• W3088334671 hasConcept C121608353 @default.
• W3088334671 hasConcept C126322002 @default.
• W3088334671 hasConcept C142724271 @default.
• W3088334671 hasConcept C178790620 @default.
• W3088334671 hasConcept C184651966 @default.
• W3088334671 hasConcept C185592680 @default.
• W3088334671 hasConcept C192562407 @default.
• W3088334671 hasConcept C204787440 @default.
• W3088334671 hasConcept C2776694085 @default.
• W3088334671 hasConcept C2778858181 @default.
• W3088334671 hasConcept C2781303535 @default.
• W3088334671 hasConcept C521977710 @default.
• W3088334671 hasConcept C71924100 @default.
• W3088334671 hasConcept C98274493 @default.
• W3088334671 hasConceptScore W3088334671C112613896 @default.
• W3088334671 hasConceptScore W3088334671C11268172 @default.
• W3088334671 hasConceptScore W3088334671C121608353 @default.
• W3088334671 hasConceptScore W3088334671C126322002 @default.
• W3088334671 hasConceptScore W3088334671C142724271 @default.
• W3088334671 hasConceptScore W3088334671C178790620 @default.
• W3088334671 hasConceptScore W3088334671C184651966 @default.
• W3088334671 hasConceptScore W3088334671C185592680 @default.
• W3088334671 hasConceptScore W3088334671C192562407 @default.
• W3088334671 hasConceptScore W3088334671C204787440 @default.
• W3088334671 hasConceptScore W3088334671C2776694085 @default.
• W3088334671 hasConceptScore W3088334671C2778858181 @default.
• W3088334671 hasConceptScore W3088334671C2781303535 @default.
• W3088334671 hasConceptScore W3088334671C521977710 @default.
• W3088334671 hasConceptScore W3088334671C71924100 @default.
• W3088334671 hasConceptScore W3088334671C98274493 @default.
• W3088334671 hasFunder F4320320721 @default.
• W3088334671 hasFunder F4320323670 @default.
• W3088334671 hasFunder F4320334771 @default.
• W3088334671 hasLocation W30883346711 @default.
• W3088334671 hasOpenAccess W3088334671 @default.
• W3088334671 hasPrimaryLocation W30883346711 @default.
• W3088334671 hasRelatedWork W1872428248 @default.
• W3088334671 hasRelatedWork W2032664996 @default.
• W3088334671 hasRelatedWork W2050559479 @default.

• next