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• W3088403496 abstract "The main objective of this study is to investigate the antibacterial activity of silver nanoparticles (AgNPs) against multidrug-resistant Salmonella isolates recovered from diarrheic sheep and goats.This study used chemical reduction synthesis of AgNPs to evaluate their antimicrobial effects by estimation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for each isolate using the microplate dilution method and tetrazolium salt reduction test to detect the viability percentage. In vivo treatment efficacy was assessed in mice by determining the viable count of Salmonella Enteritidis recovered from feces and by hematologic, biochemical and histopathologic examinations to confirm that use of AgNPs has no toxic or pathologic effects and to evaluate its ability in tissue regeneration following treatment.All recovered strains were identified as MDR with a prevalence of 4% and 3.6% in sheep and goats, respectively. The results of TEM, DLS, Zeta potential, and FTIR revealed typical characteristics of the synthesized AgNPs. Silver nanoparticles showed antibacterial activity against all recovered strains with MIC of ≤0.02-0.313 μg/mL (mean average 0.085±0.126 μg/mL) and MBC of 0.078-1.250 μg/mL (average 0.508±0.315 μg/mL). In vivo efficacy of AgNPs was observed by a reduction in the number of viable S. Enteritidis recovered from feces in an S. Enteritidis infected mouse model, with complete shedding stopping between treatment days 4 and 6. Hematologic, serum biochemical, and histopathologic analyses proved the ability of AgNPs to suppress inflammatory reaction caused by S. Enteritidis infection.The study proved the effective ability of AgNPs to fight MDR Salmonella spp. in vitro and in vivo without adverse effects." @default.
• W3088403496 created "2020-10-01" @default.
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• W3088403496 date "2020-09-01" @default.
• W3088403496 modified "2023-10-15" @default.
• W3088403496 title "<p>The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-Resistant <em>Salmonella</em> Species Isolates</p>" @default.
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• W3088403496 doi "https://doi.org/10.2147/ijn.s270204" @default.
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