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• W3088548618 abstract "Abstract Background Glioblastoma is the most common primary malignant intracranial tumor with poor clinical prognosis in adults. Accumulating evidence indicates that long non-coding RNAs (lncRNAs) function as important regulators in cancer progression, including glioblastoma. Here, we identified a new lncRNA LPP antisense RNA-2 (LPP-AS2) and investigated its function and mechanism in the development of glioma. Methods High-throughput RNA sequencing was performed to discriminate differentially expressed lncRNAs and mRNAs between glioma tissues and normal brain tissues. Expression of LPP-AS2, epidermal growth factor receptor (EGFR) and miR-7-5p in glioma tissues and cell lines was detected by real-time quantitative PCR (RT-qPCR), and the functions of lncRNA LPP-AS2 in glioma were assessed by in vivo and in vitro assays. Insight into the underlying mechanism of competitive endogenous RNAs (ceRNAs) was obtained via bioinformatic analysis, dual luciferase reporter assays, RNA pulldown assays, RNA immunoprecipitation (RIP) and rescue experiments. Results The results of high-throughput RNA-seq indicated lncRNA LPP-AS2 was upregulated in glioma tissues and further confirmed by RT-qPCR. Higher LPP-AS2 expression was related to a poor prognosis in glioma patients. Based on functional studies, LPP-AS2 depletion inhibited glioma cell proliferation, invasion and promoted apoptosis in vitro and restrained tumor growth in vivo, overexpression of LPP-AS2 resulted in the opposite effects. In addition, LPP-AS2 and EGFR were observed in co-expression networks. LPP-AS2 was found to function as a ceRNA to regulate EGFR expression by sponging miR-7-5p in glioma cells. The result of chromatin immunoprecipitation (ChIP) assays validated that c-MYC binds directly to the promoter region of LPP-AS2. As a downstream protein of EGFR, c-MYC was modulated by LPP-AS2 and in turn enhanced LPP-AS2 expression. Thus, lncRNA LPP-AS2 promoted glioma tumorigenesis via a miR-7-5p/EGFR/PI3K/AKT/c-MYC feedback loop. Conclusions Our study elucidated that LPP-AS2 acted as an oncogene through a novel molecular pathway in glioma and might be a potential therapeutic approach for glioma diagnosis, therapy and prognosis." @default.
• W3088548618 created "2020-10-01" @default.
• W3088548618 creator A5008973325 @default.
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• W3088548618 date "2020-09-22" @default.
• W3088548618 modified "2023-10-15" @default.
• W3088548618 title "Long non-coding RNA LPP-AS2 promotes glioma tumorigenesis via miR-7-5p/EGFR/PI3K/AKT/c-MYC feedback loop" @default.
• W3088548618 cites W144423133 @default.
• W3088548618 cites W1792828569 @default.
• W3088548618 cites W1831063376 @default.
• W3088548618 cites W1964444913 @default.
• W3088548618 cites W1966411071 @default.
• W3088548618 cites W1970741980 @default.
• W3088548618 cites W1985928174 @default.
• W3088548618 cites W2006288539 @default.
• W3088548618 cites W2011912935 @default.
• W3088548618 cites W2046387002 @default.
• W3088548618 cites W2070011883 @default.
• W3088548618 cites W2087377443 @default.
• W3088548618 cites W2087616533 @default.
• W3088548618 cites W2092677877 @default.
• W3088548618 cites W2102641687 @default.
• W3088548618 cites W2105349784 @default.
• W3088548618 cites W2115862526 @default.
• W3088548618 cites W2126504590 @default.
• W3088548618 cites W2137343216 @default.
• W3088548618 cites W2138541072 @default.
• W3088548618 cites W2144137532 @default.
• W3088548618 cites W2146295863 @default.
• W3088548618 cites W2153268494 @default.
• W3088548618 cites W2158217645 @default.
• W3088548618 cites W2162673078 @default.
• W3088548618 cites W2289602271 @default.
• W3088548618 cites W2300041181 @default.
• W3088548618 cites W2311921258 @default.
• W3088548618 cites W2341291028 @default.
• W3088548618 cites W2395887422 @default.
• W3088548618 cites W2411895718 @default.
• W3088548618 cites W2471861710 @default.
• W3088548618 cites W2476629295 @default.
• W3088548618 cites W2505384565 @default.
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• W3088548618 cites W2528536314 @default.
• W3088548618 cites W2542315333 @default.
• W3088548618 cites W2591074529 @default.
• W3088548618 cites W2602182609 @default.
• W3088548618 cites W2605206394 @default.
• W3088548618 cites W2607129810 @default.
• W3088548618 cites W2609243345 @default.
• W3088548618 cites W2612812586 @default.
• W3088548618 cites W2735329000 @default.
• W3088548618 cites W2749611213 @default.
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• W3088548618 cites W2767506536 @default.
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• W3088548618 cites W2770661082 @default.
• W3088548618 cites W2783798065 @default.
• W3088548618 cites W2786992500 @default.
• W3088548618 cites W2787520936 @default.
• W3088548618 cites W2789269408 @default.
• W3088548618 cites W2789382357 @default.
• W3088548618 cites W2799683421 @default.
• W3088548618 cites W2800290862 @default.
• W3088548618 cites W2886329331 @default.
• W3088548618 cites W2888150662 @default.
• W3088548618 cites W2888170117 @default.
• W3088548618 cites W2895938244 @default.
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• W3088548618 cites W2913336364 @default.
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• W3088548618 cites W2922330509 @default.
• W3088548618 cites W2951344785 @default.
• W3088548618 cites W2952632749 @default.
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• W3088548618 cites W2971469357 @default.
• W3088548618 cites W2972820322 @default.
• W3088548618 cites W2976750539 @default.
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• W3088548618 doi "https://doi.org/10.1186/s13046-020-01695-8" @default.
• W3088548618 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7510091" @default.
• W3088548618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32962742" @default.
• W3088548618 hasPublicationYear "2020" @default.
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