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- W3088553402 abstract "Abstract The aim of this study is to assess the cytoprotection and potential molecular mechanisms of procyanidin B 2 (PCB 2 ) on hydrogen peroxide (H 2 O 2 )-induced oxidative damage in MCF-7 cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the viability of MCF-7 cell exposure to H 2 O 2 or PCB 2 . We measured the antioxidant properties of PCB 2 by determining the activities of SOD, GSH-Px, LDH and MDA levels, and evaluated apoptosis and intracellular reactive oxygen species (ROS) levels. The related proteins expression levels were monitored by Western blot. MCF-7 cells induced with H 2 O 2 had a remarkable decrease in cell viability that was suppressed when it was interfered with PCB 2 (0.1–10.0 μM). PCB 2 interference memorably and dose-dependently inhibited H 2 O 2 -induced LDH leakage, ROS and MDA overproduction, while PCB 2 markedly increased H 2 O 2 -induced the activities of SOD and GSH-Px. Eventually, H 2 O 2 prominently down-regulated the ratio of Bcl-2/Bax and the relative proteins expression levels of Nrf2, GCLC, NQO1 and HO-1, and up-regulated the relative proteins expression levels of cytochrome c, caspase-3 and Keap1. However, the relative expression levels of these proteins were reversed in PCB 2 -interfered MCF-7 cells. This study implied that protective effect of PCB 2 on H 2 O 2 -induced oxidative damage in MCF-7 cells might be related to inhibition of mitochondria-dependent apoptosis, activation of Keap1/Nrf2/HO-1 signaling pathway and improvement of the antioxidant enzymes activities." @default.
- W3088553402 created "2020-10-01" @default.
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- W3088553402 date "2020-09-21" @default.
- W3088553402 modified "2023-09-23" @default.
- W3088553402 title "Protective effect of procyanidin B2 on hydrogen peroxide (H2O2)-induced oxidative damage in MCF-7 cells" @default.
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- W3088553402 doi "https://doi.org/10.1186/s13765-020-00545-7" @default.
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