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• W3088558246 abstract "Due to the ever-increasing resolution of 3D printing technology, additive manufacturing is now even used to produce complex devices for laboratory applications. Personalized experimental devices or entire cultivation systems of almost unlimited complexity can potentially be manufactured within hours from start to finish-an enormous potential for experimental parallelization in a highly controllable environment. This study presents customized 3D-printed co-cultivation systems, which qualify for angiogenesis studies. In these systems, endothelial and mesenchymal stem cells (AD-MSC) were indirectly co-cultivated-that is, both cell types were physically separated through a rigid, 3D-printed barrier in the middle, while still sharing the same cell culture medium that allows for the exchange of signalling molecules. Biochemical-based cytotoxicity assays initially confirmed that the 3D printing material does not exert any negative effects on cells. Since the material also enables phase contrast and fluorescence microscopy, the behaviour of cells could be observed over the entire cultivation via both. Microscopic observations and subsequent quantitative analysis revealed that endothelial cells form tubular-like structures as angiogenic feature when indirectly co-cultured alongside AD-MSCs in the 3D-printed co-cultivation system. In addition, further 3D-printed devices are also introduced that address different issues and aspire to help in varying experimental setups. Our results mark an important step forward for the integration of customized 3D-printed systems as self-contained test systems or equipment in biomedical applications." @default.
• W3088558246 created "2020-10-01" @default.
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• W3088558246 date "2020-09-25" @default.
• W3088558246 modified "2023-10-18" @default.
• W3088558246 title "Customizable 3D-Printed (Co-)Cultivation Systems for In Vitro Study of Angiogenesis" @default.
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• W3088558246 doi "https://doi.org/10.3390/ma13194290" @default.
• W3088558246 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7579111" @default.
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• W3088558246 hasPublicationYear "2020" @default.
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