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- W3088559610 abstract "Abstract Developing methods for accurate detection of RNA modifications remains a major challenge in epitranscriptomics. Next-generation sequencing-based mapping approaches have recently emerged but, often, they are not quantitative and lack specificity. Pseudouridine (ψ), produced by uridine isomerization, is one of the most abundant RNA modification. ψ mapping classically involves derivatization with soluble carbodiimide (CMCT), which is prone to variation making this approach only semi-quantitative. Here, we developed ‘HydraPsiSeq’, a novel quantitative ψ mapping technique relying on specific protection from hydrazine/aniline cleavage. HydraPsiSeq is quantitative because the obtained signal directly reflects pseudouridine level. Furthermore, normalization to natural unmodified RNA and/or to synthetic in vitro transcripts allows absolute measurements of modification levels. HydraPsiSeq requires minute amounts of RNA (as low as 10–50 ng), making it compatible with high-throughput profiling of diverse biological and clinical samples. Exploring the potential of HydraPsiSeq, we profiled human rRNAs, revealing strong variations in pseudouridylation levels at ∼20–25 positions out of total 104 sites. We also observed the dynamics of rRNA pseudouridylation throughout chondrogenic differentiation of human bone marrow stem cells. In conclusion, HydraPsiSeq is a robust approach for the systematic mapping and accurate quantification of pseudouridines in RNAs with applications in disease, aging, development, differentiation and/or stress response." @default.
- W3088559610 created "2020-10-01" @default.
- W3088559610 creator A5001477363 @default.
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- W3088559610 creator A5089150142 @default.
- W3088559610 date "2020-09-25" @default.
- W3088559610 modified "2023-10-18" @default.
- W3088559610 title "HydraPsiSeq: a method for systematic and quantitative mapping of pseudouridines in RNA" @default.
- W3088559610 cites W1429519121 @default.
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- W3088559610 cites W1535850881 @default.
- W3088559610 cites W1566729113 @default.
- W3088559610 cites W1824644573 @default.
- W3088559610 cites W1989849863 @default.
- W3088559610 cites W1991154121 @default.
- W3088559610 cites W1992817900 @default.
- W3088559610 cites W1998916320 @default.
- W3088559610 cites W2008121312 @default.
- W3088559610 cites W2012690689 @default.
- W3088559610 cites W2019427404 @default.
- W3088559610 cites W2020747171 @default.
- W3088559610 cites W2031000216 @default.
- W3088559610 cites W2032328269 @default.
- W3088559610 cites W2043165339 @default.
- W3088559610 cites W2044980059 @default.
- W3088559610 cites W2053212888 @default.
- W3088559610 cites W2056032938 @default.
- W3088559610 cites W2058123073 @default.
- W3088559610 cites W2058767191 @default.
- W3088559610 cites W2061440884 @default.
- W3088559610 cites W2065847836 @default.
- W3088559610 cites W2078964320 @default.
- W3088559610 cites W2083537672 @default.
- W3088559610 cites W2085928743 @default.
- W3088559610 cites W2091464648 @default.
- W3088559610 cites W2097603183 @default.
- W3088559610 cites W2105938928 @default.
- W3088559610 cites W2112093431 @default.
- W3088559610 cites W2113738713 @default.
- W3088559610 cites W2119563195 @default.
- W3088559610 cites W2120276342 @default.
- W3088559610 cites W2126756655 @default.
- W3088559610 cites W2131271579 @default.
- W3088559610 cites W2147329079 @default.
- W3088559610 cites W2154941581 @default.
- W3088559610 cites W2161890613 @default.
- W3088559610 cites W2170551349 @default.
- W3088559610 cites W2253835652 @default.
- W3088559610 cites W2313435036 @default.
- W3088559610 cites W2414460090 @default.
- W3088559610 cites W2432150382 @default.
- W3088559610 cites W2461624292 @default.
- W3088559610 cites W2467082561 @default.
- W3088559610 cites W2468350979 @default.
- W3088559610 cites W2544285983 @default.
- W3088559610 cites W2565793297 @default.
- W3088559610 cites W2566723880 @default.
- W3088559610 cites W2572064190 @default.
- W3088559610 cites W2586073275 @default.
- W3088559610 cites W2590034108 @default.
- W3088559610 cites W2600070854 @default.
- W3088559610 cites W2600492947 @default.
- W3088559610 cites W2612694788 @default.
- W3088559610 cites W2612853933 @default.
- W3088559610 cites W2754113619 @default.
- W3088559610 cites W2766140892 @default.
- W3088559610 cites W2767790244 @default.
- W3088559610 cites W2768377421 @default.
- W3088559610 cites W2794431611 @default.
- W3088559610 cites W2815388331 @default.
- W3088559610 cites W2890330353 @default.
- W3088559610 cites W2891167139 @default.
- W3088559610 cites W2898317540 @default.
- W3088559610 cites W2899193720 @default.
- W3088559610 cites W2900280264 @default.
- W3088559610 cites W2911081018 @default.
- W3088559610 cites W2923706270 @default.
- W3088559610 cites W2948860462 @default.
- W3088559610 cites W2949564046 @default.
- W3088559610 cites W2951692245 @default.
- W3088559610 cites W2971413301 @default.
- W3088559610 cites W2971578544 @default.
- W3088559610 cites W2972933339 @default.
- W3088559610 cites W3006558261 @default.
- W3088559610 cites W367446868 @default.
- W3088559610 cites W4252834064 @default.
- W3088559610 doi "https://doi.org/10.1093/nar/gkaa769" @default.
- W3088559610 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7641733" @default.
- W3088559610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32976574" @default.
- W3088559610 hasPublicationYear "2020" @default.