FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3088794562> ?p ?o ?g. }

• W3088794562 endingPage "487" @default.
• W3088794562 startingPage "487" @default.
• W3088794562 abstract "Penicillium crustosum Thom. is a fungus commonly found on cheese and nuts, but is also a postharvest pathogen that causes blue mold disease of pome and stone fruits including plum and nectarine (Louw and Korsten 2016; Restuccia et al. 2006). The fungus produces mycotoxins (penitrem A, roquefortine C, terrestric acid, and cyclopenol) which are of concern for human health (Frisvad and Samson 2004). In Serbia, P. crustosum has been previously described on apple fruit (Vico et al. 2014). On nectarine fruit (Prunus persica var. nucipersica), after 6 weeks of cold storage, symptoms of blue mold developed in a fruit market in Belgrade, Serbia. The fruit was collected and isolations performed in November 2017. Decayed areas on infected fruit were soft, light to medium brown with blue-green sporulation on the fruit surface. Two isolates were obtained (N2AS and N2BS) and cultured on Czapek yeast autolysate agar (CYA), malt extract agar (MEA), yeast extract sucrose agar (YES) and potato dextrose agar (PDA) at 25°C for 7 days. Isolates were identified as P. crustosum based on morphological features (Frisvad and Samson 2004; Pitt and Hocking 2009). On all media, mycelia were white and colonies turned blue-green with abundant sporulation. Colonies of both isolates were radially sulcate on MEA and YES, and plane with a granular texture on CYA and PDA, and were yellow to orange on the reverse side on YES. Mean colony diameter on PDA was 29.2 ± 1.2 mm for N2AS, and 31.3 ± 1.4 mm for N2BS; on CYA 30.8 ± 1.2 mm for N2AS and 30.9 ± 1.1 mm for N2BS; on YES 40.7 ± 3.6 mm for N2AS and 43.6 ± 1.4 mm for N2BS; and on MEA 33.4 ± 1.2 mm for N2AS and 34 ± 2.5 mm for N2BS. Crusts of conidial masses formed on MEA and PDA after 10 days. Conidiophores of both isolates were terverticillate, stipes were septate with rough walls, and conidia, borne in columns, were smooth and spherical to subglobose. Conidial diameter for N2AS was 2.32 to 3.95 (average 3.13) µm and for N2BS was 2.34 to 3.98 (average 3.27) µm (n=50). Isolates formed a yellow ring, using Ehrlich's reagent, indicating lack of cyclopiazonic acid, but production of other alkaloids. Morphological identification was confirmed by isolating genomic DNA, PCR amplification of the partial β-tubulin gene using Bt2a/Bt2b (Glass and Donaldson 1995) and sequencing. BLAST analysis revealed that N2AS sequence (MT799805) was 99% similar and N2BS (MT799806) was identical to sequences AY674351 (strain CBS 101025) and KJ775121 (strain DTO_244H8) of P. crustosum in GenBank. Sequences (2X consensus) of the two isolates differed in one nucleotide showing the existence of single-nucleotide polymorphism among P. crustosum isolates. Pathogenicity was tested on nectarine, peach and apple fruit (four fruit per isolate and the control). Fruit were washed, surface-sanitized with 70% ethanol, and wound (10x4 mm) inoculated on two sides with 40 μl of a 105/ml conidial suspension in sterile distilled water containing 0.1% Tween 20 (TSDW). Control fruit was inoculated with TSDW. Inoculated and control fruit were stored at 25°C for 7 days. Inoculated fruit developed light brown decay with cracks in the epidermis that spread from the inoculation point on nectarines and peaches. Blue-green sporulation was present on all inoculated fruit. Control fruit remained symptomless. The fungus was re-isolated and was morphologically identical to the original isolates, thus completing Koch's postulates. This is the first report of P. crustosum causing postharvest blue mold decay on nectarine fruit in Serbia. Results show that P. crustosum is not only present as a postharvest pathogen of apple fruit, but of nectarine as well and may pose a threat in storage of both pome and stone fruits in Serbia. References: Frisvad, J. C. and Samson, R. A. 2004. Stud. Mycol. 49:1. Glass, N.L. and Donaldson, G. C. 1995. Appl. Environ. Microbiol. 61: 1323. Louw, J.P., and Korsten, L. 2016. Eur. J. Plant Pathol. 146: 779. Pitt, J. I. and Hocking, A. D. 2009. Fungi and food spoilage, 239. Springer. Restuccia et al. 2006. J. Food Prot. 69: 2465. Vico, I., et al. 2014. Plant Dis. 98:1430. Acknowledgment: This research was supported by the project III46008, No. 451-03-68/2020-14/200116, financed by the Ministry of Education, Science and Technological Development, Republic of Serbia." @default.
• W3088794562 created "2020-10-01" @default.
• W3088794562 creator A5023207720 @default.
• W3088794562 creator A5024770966 @default.
• W3088794562 creator A5039891369 @default.
• W3088794562 creator A5075518449 @default.
• W3088794562 creator A5080525411 @default.
• W3088794562 date "2021-02-01" @default.
• W3088794562 modified "2023-10-16" @default.
• W3088794562 title "First Report of Blue Mold Caused by <i>Penicillium crustosum</i> on Nectarine Fruit in Serbia" @default.
• W3088794562 cites W1869907716 @default.
• W3088794562 cites W2103610678 @default.
• W3088794562 cites W2397418336 @default.
• W3088794562 cites W4230171876 @default.
• W3088794562 cites W4254411246 @default.
• W3088794562 cites W73101576 @default.
• W3088794562 doi "https://doi.org/10.1094/pdis-07-20-1632-pdn" @default.
• W3088794562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32967562" @default.
• W3088794562 hasPublicationYear "2021" @default.
• W3088794562 type Work @default.
• W3088794562 sameAs 3088794562 @default.
• W3088794562 citedByCount "4" @default.
• W3088794562 countsByYear W30887945622021 @default.
• W3088794562 countsByYear W30887945622022 @default.
• W3088794562 crossrefType "journal-article" @default.
• W3088794562 hasAuthorship W3088794562A5023207720 @default.
• W3088794562 hasAuthorship W3088794562A5024770966 @default.
• W3088794562 hasAuthorship W3088794562A5039891369 @default.
• W3088794562 hasAuthorship W3088794562A5075518449 @default.
• W3088794562 hasAuthorship W3088794562A5080525411 @default.
• W3088794562 hasBestOaLocation W30887945621 @default.
• W3088794562 hasConcept C143108565 @default.
• W3088794562 hasConcept C144027150 @default.
• W3088794562 hasConcept C157670687 @default.
• W3088794562 hasConcept C2777484150 @default.
• W3088794562 hasConcept C2778138406 @default.
• W3088794562 hasConcept C2778660310 @default.
• W3088794562 hasConcept C2779692143 @default.
• W3088794562 hasConcept C2779951321 @default.
• W3088794562 hasConcept C2780630512 @default.
• W3088794562 hasConcept C2781240563 @default.
• W3088794562 hasConcept C523546767 @default.
• W3088794562 hasConcept C54355233 @default.
• W3088794562 hasConcept C59822182 @default.
• W3088794562 hasConcept C83082669 @default.
• W3088794562 hasConcept C86803240 @default.
• W3088794562 hasConceptScore W3088794562C143108565 @default.
• W3088794562 hasConceptScore W3088794562C144027150 @default.
• W3088794562 hasConceptScore W3088794562C157670687 @default.
• W3088794562 hasConceptScore W3088794562C2777484150 @default.
• W3088794562 hasConceptScore W3088794562C2778138406 @default.
• W3088794562 hasConceptScore W3088794562C2778660310 @default.
• W3088794562 hasConceptScore W3088794562C2779692143 @default.
• W3088794562 hasConceptScore W3088794562C2779951321 @default.
• W3088794562 hasConceptScore W3088794562C2780630512 @default.
• W3088794562 hasConceptScore W3088794562C2781240563 @default.
• W3088794562 hasConceptScore W3088794562C523546767 @default.
• W3088794562 hasConceptScore W3088794562C54355233 @default.
• W3088794562 hasConceptScore W3088794562C59822182 @default.
• W3088794562 hasConceptScore W3088794562C83082669 @default.
• W3088794562 hasConceptScore W3088794562C86803240 @default.
• W3088794562 hasFunder F4320322729 @default.
• W3088794562 hasIssue "2" @default.
• W3088794562 hasLocation W30887945621 @default.
• W3088794562 hasLocation W30887945622 @default.
• W3088794562 hasOpenAccess W3088794562 @default.
• W3088794562 hasPrimaryLocation W30887945621 @default.
• W3088794562 hasRelatedWork W1969028531 @default.
• W3088794562 hasRelatedWork W2006450668 @default.
• W3088794562 hasRelatedWork W2041575431 @default.
• W3088794562 hasRelatedWork W2058188979 @default.
• W3088794562 hasRelatedWork W2068803650 @default.
• W3088794562 hasRelatedWork W2124495075 @default.
• W3088794562 hasRelatedWork W2339954562 @default.
• W3088794562 hasRelatedWork W2397418336 @default.
• W3088794562 hasRelatedWork W2980662857 @default.
• W3088794562 hasRelatedWork W3166612526 @default.
• W3088794562 hasVolume "105" @default.
• W3088794562 isParatext "false" @default.
• W3088794562 isRetracted "false" @default.
• W3088794562 magId "3088794562" @default.
• W3088794562 workType "article" @default.