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- W3089098168 abstract "Human milk oligosaccharides (HMOs) are unique components of human breast milk. Their large-scale production by fermentation allows infant formulas to be fortified with HMOs, but current fermentation processes require lactose as a starting material, increasing the costs, bioburden, and environmental impact of manufacturing. Here we report the development of an Escherichia coli strain that produces 2′-fucosyllactose (2′-FL), the most abundant HMO, de novo using sucrose as the sole carbon source. Strain engineering required the expression of a novel glucose-accepting galactosyltransferase, overexpression of the de novo UDP-d-galactose and GDP-l-fucose pathways, the engineering of an intracellular pool of free glucose, and overexpression of a suitable α(1,2)-fucosyltransferase. The export of 2′-FL was facilitated using a sugar efflux transporter. The final production strain achieved 2′-FL yields exceeding 60 g/L after fermentation for 84 h. This efficient strategy facilitates the lactose-independent production of HMOs by fermentation, which will improve product quality and reduce the costs of manufacturing." @default.
- W3089098168 created "2020-10-01" @default.
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- W3089098168 date "2020-09-23" @default.
- W3089098168 modified "2023-10-05" @default.
- W3089098168 title "High-Titer <i>De Novo</i> Biosynthesis of the Predominant Human Milk Oligosaccharide 2′-Fucosyllactose from Sucrose in <i>Escherichia coli</i>" @default.
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- W3089098168 doi "https://doi.org/10.1021/acssynbio.0c00304" @default.
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