Matches in SemOpenAlex for { <https://semopenalex.org/work/W3089248794> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W3089248794 endingPage "612" @default.
- W3089248794 startingPage "607" @default.
- W3089248794 abstract "Abstract Human high temperature requirement protease A2 (HtrA2) is a trimeric PDZ bearing proapoptotic serine protease, which is involved in various cellular processes and pathologies. Research in the last decade strongly advocates its role as a potential therapeutic target and therefore warrants the need to minutely investigate its mechanism of action, regulation, interactions with other proteins and its binding specificities. In this particular study, we adopted an in silico approach to predict novel interacting partners and/or substrates of HtrA2 by building a peptide library using a binding pattern search. This library was used to look for novel ligand proteins in the human proteome. Thereafter, the putative interaction was validated using biochemical and cell-based studies. In a first, here we report that HtrA2 shows robust interactions with DUSP9 (Dual specificity phosphatase 9) in GST-pulldown and Co-Immunoprecipitation (Co-IP) experiments and cleaves it in vitro. Besides, we also provided a detailed characterization of the interaction interface. Moreover, this study in general provides an efficient, fast and practical method of candidate ligand library screening for exploring the binding properties of HtrA2." @default.
- W3089248794 created "2020-10-01" @default.
- W3089248794 creator A5029623278 @default.
- W3089248794 creator A5059335539 @default.
- W3089248794 creator A5064388098 @default.
- W3089248794 creator A5064465717 @default.
- W3089248794 date "2020-12-01" @default.
- W3089248794 modified "2023-10-16" @default.
- W3089248794 title "Dual specificity phosphatase 9: A novel binding partner cum substrate of proapoptotic serine protease HtrA2" @default.
- W3089248794 cites W1031578623 @default.
- W3089248794 cites W1519752295 @default.
- W3089248794 cites W1547142901 @default.
- W3089248794 cites W1964834931 @default.
- W3089248794 cites W1987134040 @default.
- W3089248794 cites W2000769711 @default.
- W3089248794 cites W2001859505 @default.
- W3089248794 cites W2008574867 @default.
- W3089248794 cites W2021546591 @default.
- W3089248794 cites W2047449144 @default.
- W3089248794 cites W2055043387 @default.
- W3089248794 cites W2058243732 @default.
- W3089248794 cites W2058309041 @default.
- W3089248794 cites W2060079863 @default.
- W3089248794 cites W2060470131 @default.
- W3089248794 cites W2096209101 @default.
- W3089248794 cites W2141732393 @default.
- W3089248794 cites W2142678478 @default.
- W3089248794 cites W2147787249 @default.
- W3089248794 cites W2152458080 @default.
- W3089248794 cites W2156043727 @default.
- W3089248794 cites W2158714788 @default.
- W3089248794 cites W2239096611 @default.
- W3089248794 cites W2751476102 @default.
- W3089248794 cites W4244304233 @default.
- W3089248794 doi "https://doi.org/10.1016/j.bbrc.2020.09.062" @default.
- W3089248794 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32988583" @default.
- W3089248794 hasPublicationYear "2020" @default.
- W3089248794 type Work @default.
- W3089248794 sameAs 3089248794 @default.
- W3089248794 citedByCount "5" @default.
- W3089248794 countsByYear W30892487942022 @default.
- W3089248794 countsByYear W30892487942023 @default.
- W3089248794 crossrefType "journal-article" @default.
- W3089248794 hasAuthorship W3089248794A5029623278 @default.
- W3089248794 hasAuthorship W3089248794A5059335539 @default.
- W3089248794 hasAuthorship W3089248794A5064388098 @default.
- W3089248794 hasAuthorship W3089248794A5064465717 @default.
- W3089248794 hasConcept C138885662 @default.
- W3089248794 hasConcept C153911025 @default.
- W3089248794 hasConcept C178666793 @default.
- W3089248794 hasConcept C181199279 @default.
- W3089248794 hasConcept C185592680 @default.
- W3089248794 hasConcept C2776414213 @default.
- W3089248794 hasConcept C2776714187 @default.
- W3089248794 hasConcept C2777807008 @default.
- W3089248794 hasConcept C2780429522 @default.
- W3089248794 hasConcept C2780980858 @default.
- W3089248794 hasConcept C2994592520 @default.
- W3089248794 hasConcept C41895202 @default.
- W3089248794 hasConcept C55493867 @default.
- W3089248794 hasConcept C86803240 @default.
- W3089248794 hasConceptScore W3089248794C138885662 @default.
- W3089248794 hasConceptScore W3089248794C153911025 @default.
- W3089248794 hasConceptScore W3089248794C178666793 @default.
- W3089248794 hasConceptScore W3089248794C181199279 @default.
- W3089248794 hasConceptScore W3089248794C185592680 @default.
- W3089248794 hasConceptScore W3089248794C2776414213 @default.
- W3089248794 hasConceptScore W3089248794C2776714187 @default.
- W3089248794 hasConceptScore W3089248794C2777807008 @default.
- W3089248794 hasConceptScore W3089248794C2780429522 @default.
- W3089248794 hasConceptScore W3089248794C2780980858 @default.
- W3089248794 hasConceptScore W3089248794C2994592520 @default.
- W3089248794 hasConceptScore W3089248794C41895202 @default.
- W3089248794 hasConceptScore W3089248794C55493867 @default.
- W3089248794 hasConceptScore W3089248794C86803240 @default.
- W3089248794 hasIssue "3" @default.
- W3089248794 hasLocation W30892487941 @default.
- W3089248794 hasOpenAccess W3089248794 @default.
- W3089248794 hasPrimaryLocation W30892487941 @default.
- W3089248794 hasRelatedWork W109123086 @default.
- W3089248794 hasRelatedWork W2011904545 @default.
- W3089248794 hasRelatedWork W2029174463 @default.
- W3089248794 hasRelatedWork W2036951984 @default.
- W3089248794 hasRelatedWork W2056391400 @default.
- W3089248794 hasRelatedWork W2058759516 @default.
- W3089248794 hasRelatedWork W2080463731 @default.
- W3089248794 hasRelatedWork W2080509753 @default.
- W3089248794 hasRelatedWork W2090010218 @default.
- W3089248794 hasRelatedWork W2491451478 @default.
- W3089248794 hasVolume "533" @default.
- W3089248794 isParatext "false" @default.
- W3089248794 isRetracted "false" @default.
- W3089248794 magId "3089248794" @default.
- W3089248794 workType "article" @default.