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- W3089357170 abstract "This study was aimed to increase the production of Spilanthol from S. acmella by using tissue cultures.Seeds were sterilized and cultured on MS medium supplemented with different concentrations 2.0,4.0,6.0mg/l of Naphthalene acetic acid (NAA),1.0 , 2.0 , 3.0 mg/l Benzyl adenine (BA)and 1.0, 2.0, 3.0 mg/l Benzylamino purine (BAP). These growth regulaters were used for callus induction from leaf segments, plus 0.1gm casein hydrolysate in combination with the same growth regulaters for callus induction and Spilantholproduction. Quality and quantity of Spilanthol were investingated using high performance liquidchromatography (HPLC), results showed that using 1.5% sodium hypochlorate for 15 min was veryeffective for disinfecting and survival . The combination of 3.0 mg/ l BAP, 6.0 mg/l NAA with 0.1 gm caseinhydrolysed was most effective for callus induction from leaf segments ,The highest mean fresh weight andthe highest amount of Spilanthol reached to 80.46 mg/g in combination of 2.0 mg/lBAP, 2.0 mg/l NAA with0.1 g casein hydrolysed. This increased the Spilanthol compound to its highest amount when compairedwith the original plant." @default.
- W3089357170 created "2020-10-08" @default.
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- W3089357170 date "2015-06-01" @default.
- W3089357170 modified "2023-09-25" @default.
- W3089357170 title "Increasing the Production of Spilanthol in Spilanthes acmella (L.)Murr. by Using Tissue Culture Techniques" @default.
- W3089357170 doi "https://doi.org/10.24126/jobrc.2015.9.2.431" @default.
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