FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3089476998> ?p ?o ?g. }

• W3089476998 abstract "Background Cytokine-Induced Killer (CIK) cells are ex vivo expanded T cells with NK cell phenotype. They express both CD3 and CD56 antigens, and exert a potent antitumor activity against a variety of tumors. Several clinical trials demonstrated the safety and the feasibility of CIK cell therapy, with very low side effects and minimal graft-versus-host toxicity. In this study, we developed a GMP-compliant protocol for robust large-scale expansion of CIK cells using G-Rex® gas-permeable static culture flasks. Materials and Methods CIK cells were obtained by stimulating healthy donor PBMCs with GMP-grade IFN-γ, IL-2 and CD3 mAbs, and were cultured in G-Rex6® or G-Rex®6M well plates. CIK cells in G-Rex6® were split only once at day 7 to reduce cell density, whereas the number of CIK cells culterd in G-Rex®6M was not adjusted. In both culture conditions, fresh IL-2 was provided every 3–4 days. We compared these two culture protocols with the culture in standard flasks. Phenotype was analyzed by flow cytometry and cytotoxicity was assessed against several tumor cell lines by calcein-release assay. Results CIK cells cultured in G-Rex6® well plates showed an outstanding cell expansion compared to G-Rex®6M well plates or standard culture flasks, with a 400-fold expansion and a mean of 10 9 total cells obtained per single well in 14 days, starting from just 2.5 × 10 6 cells per well. Moreover, the cultures in G-Rex6® were characterized by an higher percentage of CD3 + CD56 + cells, as compared to G-Rex®6M or standard culture flasks. Cells cultured in all devices had a comparable expression of NKG2D, NKp30, NKp44, 2B4 receptors. Importantly, CIK cells expanded in G-Rex®6 were as cytotoxic as cells expanded in standard culture flasks. Conversely, CIK cells cultured in G-Rex®6M showed a remarkable reduction of cytotoxicity against tumor cell targets, thus suggesting that cell density during expansion could affect CIK cell activity. Conclusions We propose a GMP-compliant protocol for robust large-scale production of CIK cells. G-Rex® system allows to obtain large amounts of CIK cells highly enriched in the CD3 + CD56 + subset and endowed with high cytotoxic activity; this can be accomplished with just a single cell culture split at day 7, which dramatically reduces the culture manipulation as compared to the standard culture flasks. Notably, this strategy can be further and easily scalable to produce CIK cells for clinical immunotherapy applications. Disclosure Information A. Ventura: None. P. Palmerini: None. A. Dalla Pietà: None. R. Sommaggio: None. G. Astori: None. K. Chieregato: None. M. Tisi: None. C. Visco: None. O. Perbellini: None. M. Ruggeri: None. E. Cappuzzello: None. A. Rosato: None." @default.
• W3089476998 created "2020-10-08" @default.
• W3089476998 creator A5003274863 @default.
• W3089476998 creator A5017630713 @default.
• W3089476998 creator A5027899852 @default.
• W3089476998 creator A5031434443 @default.
• W3089476998 creator A5041326568 @default.
• W3089476998 creator A5058093044 @default.
• W3089476998 creator A5059045065 @default.
• W3089476998 creator A5060753670 @default.
• W3089476998 creator A5074505517 @default.
• W3089476998 creator A5075657251 @default.
• W3089476998 creator A5078017131 @default.
• W3089476998 creator A5083158120 @default.
• W3089476998 date "2020-10-01" @default.
• W3089476998 modified "2023-09-27" @default.
• W3089476998 title "P09.13 Optimization of a GMP-grade large-scale expansion protocol for cytokine-induced killer cells using gas-permeable static culture flasks" @default.
• W3089476998 doi "https://doi.org/10.1136/jitc-2020-itoc7.113" @default.
• W3089476998 hasPublicationYear "2020" @default.
• W3089476998 type Work @default.
• W3089476998 sameAs 3089476998 @default.
• W3089476998 citedByCount "0" @default.
• W3089476998 crossrefType "journal-article" @default.
• W3089476998 hasAuthorship W3089476998A5003274863 @default.
• W3089476998 hasAuthorship W3089476998A5017630713 @default.
• W3089476998 hasAuthorship W3089476998A5027899852 @default.
• W3089476998 hasAuthorship W3089476998A5031434443 @default.
• W3089476998 hasAuthorship W3089476998A5041326568 @default.
• W3089476998 hasAuthorship W3089476998A5058093044 @default.
• W3089476998 hasAuthorship W3089476998A5059045065 @default.
• W3089476998 hasAuthorship W3089476998A5060753670 @default.
• W3089476998 hasAuthorship W3089476998A5074505517 @default.
• W3089476998 hasAuthorship W3089476998A5075657251 @default.
• W3089476998 hasAuthorship W3089476998A5078017131 @default.
• W3089476998 hasAuthorship W3089476998A5083158120 @default.
• W3089476998 hasBestOaLocation W30894769981 @default.
• W3089476998 hasConcept C114684123 @default.
• W3089476998 hasConcept C139149728 @default.
• W3089476998 hasConcept C147483822 @default.
• W3089476998 hasConcept C147789679 @default.
• W3089476998 hasConcept C153911025 @default.
• W3089476998 hasConcept C167672396 @default.
• W3089476998 hasConcept C185592680 @default.
• W3089476998 hasConcept C203014093 @default.
• W3089476998 hasConcept C2778690821 @default.
• W3089476998 hasConcept C2779109515 @default.
• W3089476998 hasConcept C54355233 @default.
• W3089476998 hasConcept C553184892 @default.
• W3089476998 hasConcept C81885089 @default.
• W3089476998 hasConcept C86803240 @default.
• W3089476998 hasConceptScore W3089476998C114684123 @default.
• W3089476998 hasConceptScore W3089476998C139149728 @default.
• W3089476998 hasConceptScore W3089476998C147483822 @default.
• W3089476998 hasConceptScore W3089476998C147789679 @default.
• W3089476998 hasConceptScore W3089476998C153911025 @default.
• W3089476998 hasConceptScore W3089476998C167672396 @default.
• W3089476998 hasConceptScore W3089476998C185592680 @default.
• W3089476998 hasConceptScore W3089476998C203014093 @default.
• W3089476998 hasConceptScore W3089476998C2778690821 @default.
• W3089476998 hasConceptScore W3089476998C2779109515 @default.
• W3089476998 hasConceptScore W3089476998C54355233 @default.
• W3089476998 hasConceptScore W3089476998C553184892 @default.
• W3089476998 hasConceptScore W3089476998C81885089 @default.
• W3089476998 hasConceptScore W3089476998C86803240 @default.
• W3089476998 hasLocation W30894769981 @default.
• W3089476998 hasOpenAccess W3089476998 @default.
• W3089476998 hasPrimaryLocation W30894769981 @default.
• W3089476998 hasRelatedWork W11803546 @default.
• W3089476998 hasRelatedWork W11833714 @default.
• W3089476998 hasRelatedWork W13811198 @default.
• W3089476998 hasRelatedWork W18310587 @default.
• W3089476998 hasRelatedWork W20179192 @default.
• W3089476998 hasRelatedWork W6139446 @default.
• W3089476998 hasRelatedWork W7601916 @default.
• W3089476998 hasRelatedWork W7899714 @default.
• W3089476998 hasRelatedWork W9256180 @default.
• W3089476998 hasRelatedWork W1847038 @default.
• W3089476998 isParatext "false" @default.
• W3089476998 isRetracted "false" @default.
• W3089476998 magId "3089476998" @default.
• W3089476998 workType "article" @default.