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- W3089485553 abstract "The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants. The RNA-guided nuclease can be used to not only generate precise genomic mutations, but also to manipulate gene expression when present as a deactivated protein (dCas9).In this study, we describe a vector toolkit for analyzing dCas9-mediated activation (CRISPRa) or inactivation (CRISPRi) of gene expression in maize protoplasts. An improved maize protoplast isolation and transfection method is presented, as well as a description of dCas9 vectors to enhance or repress maize gene expression.We anticipate that this maize protoplast toolkit will streamline the analysis of gRNA candidates and facilitate genetic studies of important trait genes in this transformation-recalcitrant plant." @default.
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- W3089485553 date "2020-10-02" @default.
- W3089485553 modified "2023-10-18" @default.
- W3089485553 title "A CRISPR/dCas9 toolkit for functional analysis of maize genes" @default.
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- W3089485553 doi "https://doi.org/10.1186/s13007-020-00675-5" @default.
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