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- W3089678834 abstract "Abstract Background The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process. Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei . Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available. A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network. Results Experimental results confirmed the impact of sugar mixture on the enzymatic cocktail composition. The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile. A gene regulatory network (GRN) built using the BRANE Cut software reveals three sub-networks related to i a positive correlation between lactose concentration and cellulase production, ii a particular dependence of the lactose onto the β -glucosidase regulation and iii a negative regulation of the development process and growth. Conclusions This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode. Our study expose a co-orchestration of xyr1 , clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β -glucosidase and a decrease of growth in favor of cellulase production. These conclusions provide us with potential targets for further genetic engineering leading to better cellulase-producing strains." @default.
- W3089678834 created "2020-10-08" @default.
- W3089678834 creator A5004177638 @default.
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- W3089678834 creator A5078683978 @default.
- W3089678834 date "2020-10-04" @default.
- W3089678834 modified "2023-09-26" @default.
- W3089678834 title "Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30" @default.
- W3089678834 cites W115123004 @default.
- W3089678834 cites W1451523330 @default.
- W3089678834 cites W1700615994 @default.
- W3089678834 cites W1791233317 @default.
- W3089678834 cites W1906461790 @default.
- W3089678834 cites W1914511025 @default.
- W3089678834 cites W1968468250 @default.
- W3089678834 cites W1970105037 @default.
- W3089678834 cites W1985405185 @default.
- W3089678834 cites W2003698297 @default.
- W3089678834 cites W2004280561 @default.
- W3089678834 cites W2012219468 @default.
- W3089678834 cites W2021738810 @default.
- W3089678834 cites W2022680565 @default.
- W3089678834 cites W2023767092 @default.
- W3089678834 cites W2026589666 @default.
- W3089678834 cites W2028168480 @default.
- W3089678834 cites W2029629283 @default.
- W3089678834 cites W2030654163 @default.
- W3089678834 cites W2030832855 @default.
- W3089678834 cites W2041830656 @default.
- W3089678834 cites W2044965911 @default.
- W3089678834 cites W2046808586 @default.
- W3089678834 cites W2047999975 @default.
- W3089678834 cites W2050074420 @default.
- W3089678834 cites W2050402774 @default.
- W3089678834 cites W2052622825 @default.
- W3089678834 cites W2068729249 @default.
- W3089678834 cites W2075743939 @default.
- W3089678834 cites W2082324597 @default.
- W3089678834 cites W2082591769 @default.
- W3089678834 cites W2089207270 @default.
- W3089678834 cites W2095361018 @default.
- W3089678834 cites W2103017472 @default.
- W3089678834 cites W2106319366 @default.
- W3089678834 cites W2106387791 @default.
- W3089678834 cites W2108234281 @default.
- W3089678834 cites W2109384743 @default.
- W3089678834 cites W2115436659 @default.
- W3089678834 cites W2116598854 @default.
- W3089678834 cites W2118481109 @default.
- W3089678834 cites W2120320979 @default.
- W3089678834 cites W2120814647 @default.
- W3089678834 cites W2123100463 @default.
- W3089678834 cites W2124985265 @default.
- W3089678834 cites W2127297472 @default.
- W3089678834 cites W2130695937 @default.
- W3089678834 cites W2134526812 @default.
- W3089678834 cites W2136292095 @default.
- W3089678834 cites W2147339485 @default.
- W3089678834 cites W2154462802 @default.
- W3089678834 cites W2155120873 @default.
- W3089678834 cites W2156426990 @default.
- W3089678834 cites W2157013134 @default.
- W3089678834 cites W2157186150 @default.
- W3089678834 cites W2157931305 @default.
- W3089678834 cites W2159269304 @default.
- W3089678834 cites W2161922735 @default.
- W3089678834 cites W2162595238 @default.
- W3089678834 cites W2165574256 @default.
- W3089678834 cites W2165763383 @default.
- W3089678834 cites W2177920816 @default.
- W3089678834 cites W2221523274 @default.
- W3089678834 cites W2304105402 @default.
- W3089678834 cites W2419653649 @default.
- W3089678834 cites W2471096355 @default.
- W3089678834 cites W2522890822 @default.
- W3089678834 cites W2527459689 @default.
- W3089678834 cites W2604229156 @default.
- W3089678834 cites W2625419514 @default.
- W3089678834 cites W2751617862 @default.
- W3089678834 cites W2756789532 @default.
- W3089678834 cites W2772432103 @default.
- W3089678834 cites W2789854595 @default.
- W3089678834 cites W2793196451 @default.
- W3089678834 cites W2838126376 @default.
- W3089678834 cites W2880740023 @default.
- W3089678834 cites W2887436780 @default.
- W3089678834 cites W2913228488 @default.
- W3089678834 cites W2937278281 @default.
- W3089678834 cites W2938451512 @default.
- W3089678834 cites W2942781309 @default.
- W3089678834 cites W2949816873 @default.
- W3089678834 cites W2950131515 @default.
- W3089678834 cites W2951675384 @default.
- W3089678834 cites W2996281985 @default.
- W3089678834 doi "https://doi.org/10.1101/2020.10.02.324319" @default.