FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3089713013> ?p ?o ?g. }

• W3089713013 endingPage "4742" @default.
• W3089713013 startingPage "4734" @default.
• W3089713013 abstract "Sulfiredoxin‑1 (SRX1) is a conserved endogenous antioxidative protein, which is involved in the response to cellular damage caused by oxidative stress. Oxidative stress and inflammation are the primary pathological changes in spinal cord injuries (SCI). The aim of present study was to explore the roles of SRX1 in SCI. Using reverse transcription‑quantitative PCR and western blotting, the present study discovered that the expression levels of SRX1 were downregulated in the spinal cord tissues of SCI model rats. Massive irregular cavities and decreased Nissl bodies were observed in the model group compared with the sham group. Thus, to determine the underlying mechanisms, neuron‑like PC12 cells were cultured in vitro. Western blotting analysis indicated that SRX1 expression levels were downregulated following the exposure of cells to lipopolysaccharide (LPS). Following the transfection with the SRX1 overexpression plasmid and stimulation with LPS, the results of the Cell Counting Kit‑8 assay indicated that the cell viability was increased compared with LPS stimulation alone. Furthermore, the expression levels of proinflammatory cytokines secreted by LPS‑treated PC12 cells were downregulated following SRX1 overexpression. Increased malondialdehyde content, decreased superoxide dismutase activity and reactive oxygen species production were also identified in PC12 cells treated with LPS using commercial detection kits, whereas the overexpression of SRX1 partially reversed the effects caused by LPS stimulation. The aforementioned results were further verified by determining the expression levels of antioxidative proteins using western blotting analysis. In addition, nuclear factor erythroid‑2‑related factor 2 (NRF2), a transcription factor known to regulate SRX1, was indicated to participate in the protective effect of SRX1 against oxidative stress. Inhibition of NRF2 further downregulated the expression levels of SRX1, NAD(P)H dehydrogenase quinone 1 and heme oxygenase‑1 in the presence of LPS, while activation of NRF2 reversed the effects of LPS on the expression levels of these proteins. In conclusion, the results of the present study indicated that the anti‑inflammatory and antioxidative effects of SRX1 may depend on NRF2, providing evidence that SRX1 may serve as a novel molecular target to exert a neuroprotective effect in SCI." @default.
• W3089713013 created "2020-10-08" @default.
• W3089713013 creator A5018729969 @default.
• W3089713013 creator A5034383217 @default.
• W3089713013 creator A5044925584 @default.
• W3089713013 creator A5057554882 @default.
• W3089713013 creator A5073863775 @default.
• W3089713013 date "2020-09-28" @default.
• W3089713013 modified "2023-10-16" @default.
• W3089713013 title "Inflammatory response and oxidative stress attenuated by sulfiredoxin‑1 in neuron‑like cells depends on nuclear factor erythroid‑2‑related factor 2" @default.
• W3089713013 cites W1506914739 @default.
• W3089713013 cites W1510295638 @default.
• W3089713013 cites W1600346125 @default.
• W3089713013 cites W1767659454 @default.
• W3089713013 cites W1967925595 @default.
• W3089713013 cites W1982192193 @default.
• W3089713013 cites W1985042590 @default.
• W3089713013 cites W2003687276 @default.
• W3089713013 cites W2006672022 @default.
• W3089713013 cites W2022697357 @default.
• W3089713013 cites W2049713754 @default.
• W3089713013 cites W2052380760 @default.
• W3089713013 cites W2077964579 @default.
• W3089713013 cites W2084141237 @default.
• W3089713013 cites W2092854379 @default.
• W3089713013 cites W2107277218 @default.
• W3089713013 cites W2128945056 @default.
• W3089713013 cites W2134705330 @default.
• W3089713013 cites W2299674453 @default.
• W3089713013 cites W2344977712 @default.
• W3089713013 cites W2475113336 @default.
• W3089713013 cites W2515257966 @default.
• W3089713013 cites W2521925016 @default.
• W3089713013 cites W2550532366 @default.
• W3089713013 cites W2581831437 @default.
• W3089713013 cites W2614737295 @default.
• W3089713013 cites W2619604815 @default.
• W3089713013 cites W2620759028 @default.
• W3089713013 cites W2757084251 @default.
• W3089713013 cites W2766209227 @default.
• W3089713013 cites W2804037648 @default.
• W3089713013 cites W2807481207 @default.
• W3089713013 cites W2888690979 @default.
• W3089713013 cites W2893614816 @default.
• W3089713013 cites W2900611411 @default.
• W3089713013 cites W2903448481 @default.
• W3089713013 cites W2903961260 @default.
• W3089713013 cites W2923507370 @default.
• W3089713013 cites W2933115845 @default.
• W3089713013 cites W2946364203 @default.
• W3089713013 cites W2984716297 @default.
• W3089713013 cites W2994651299 @default.
• W3089713013 cites W2994914254 @default.
• W3089713013 cites W3140711756 @default.
• W3089713013 doi "https://doi.org/10.3892/mmr.2020.11545" @default.
• W3089713013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7646873" @default.
• W3089713013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33173963" @default.
• W3089713013 hasPublicationYear "2020" @default.
• W3089713013 type Work @default.
• W3089713013 sameAs 3089713013 @default.
• W3089713013 citedByCount "3" @default.
• W3089713013 countsByYear W30897130132021 @default.
• W3089713013 countsByYear W30897130132022 @default.
• W3089713013 crossrefType "journal-article" @default.
• W3089713013 hasAuthorship W3089713013A5018729969 @default.
• W3089713013 hasAuthorship W3089713013A5034383217 @default.
• W3089713013 hasAuthorship W3089713013A5044925584 @default.
• W3089713013 hasAuthorship W3089713013A5057554882 @default.
• W3089713013 hasAuthorship W3089713013A5073863775 @default.
• W3089713013 hasBestOaLocation W30897130131 @default.
• W3089713013 hasConcept C185592680 @default.
• W3089713013 hasConcept C190283241 @default.
• W3089713013 hasConcept C203014093 @default.
• W3089713013 hasConcept C2776151105 @default.
• W3089713013 hasConcept C2781018059 @default.
• W3089713013 hasConcept C29537977 @default.
• W3089713013 hasConcept C502942594 @default.
• W3089713013 hasConcept C55493867 @default.
• W3089713013 hasConcept C86803240 @default.
• W3089713013 hasConcept C95444343 @default.
• W3089713013 hasConceptScore W3089713013C185592680 @default.
• W3089713013 hasConceptScore W3089713013C190283241 @default.
• W3089713013 hasConceptScore W3089713013C203014093 @default.
• W3089713013 hasConceptScore W3089713013C2776151105 @default.
• W3089713013 hasConceptScore W3089713013C2781018059 @default.
• W3089713013 hasConceptScore W3089713013C29537977 @default.
• W3089713013 hasConceptScore W3089713013C502942594 @default.
• W3089713013 hasConceptScore W3089713013C55493867 @default.
• W3089713013 hasConceptScore W3089713013C86803240 @default.
• W3089713013 hasConceptScore W3089713013C95444343 @default.
• W3089713013 hasIssue "6" @default.
• W3089713013 hasLocation W30897130131 @default.
• W3089713013 hasLocation W30897130132 @default.
• W3089713013 hasOpenAccess W3089713013 @default.
• W3089713013 hasPrimaryLocation W30897130131 @default.
• W3089713013 hasRelatedWork W2005837469 @default.
• W3089713013 hasRelatedWork W2016803845 @default.
• W3089713013 hasRelatedWork W2094452529 @default.

• next