FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3089751495> ?p ?o ?g. }

• W3089751495 abstract "Summary Leptosphaeria maculans , the causal agent of blackleg disease, interacts with Brassica napus (oilseed rape, canola) in a gene-for-gene manner. The avirulence genes AvrLmS and AvrLep2 were described to be perceived by the resistance genes RlmS and LepR2 , respectively, present in the cultivar Surpass 400. Here we report cloning of AvrLmS and AvrLep2 using two independent methods. AvrLmS was cloned using combined in vitro crossing between avirulent and virulent isolates with sequencing of DNA bulks from avirulent or virulent progeny (Bulked-Segregant-Sequencing) to rapidly identify one candidate avirulence gene present in the effector repertoire of L. maculans. AvrLep2 was cloned using a bi-parental cross of avirulent and virulent L. maculans isolates and a classical map-based cloning approach. Taking these two approaches independently, we found that AvrLmS and AvrLep2 are the same gene. Complementation of virulent isolates with this gene confirmed its role in inducing resistance on Surpass 400 and Topas- LepR2 . The gene renamed AvrLmS-Lep2 encodes for a small cysteine-rich protein of unknown function with an N-terminal secretory signal peptide, which are common features of the majority of effectors from extracellular fungal plant pathogens. The AvrLmS-Lep2 / LepR2 interaction phenotype was found to vary from a typical hypersensitive response to intermediate resistance sometimes at the edge of, or evolving toward, susceptibility depending on the inoculation conditions. AvrLmS-Lep2 was nevertheless sufficient to significantly reduce the stem lesion size on plant genotypes with LepR2 , indicating the potential efficiency of this resistance to control the disease in the field." @default.
• W3089751495 created "2020-10-08" @default.
• W3089751495 creator A5012907964 @default.
• W3089751495 creator A5013078077 @default.
• W3089751495 creator A5029142572 @default.
• W3089751495 creator A5040306269 @default.
• W3089751495 creator A5054738550 @default.
• W3089751495 creator A5056459986 @default.
• W3089751495 creator A5059081530 @default.
• W3089751495 creator A5064135644 @default.
• W3089751495 creator A5064481209 @default.
• W3089751495 creator A5068494489 @default.
• W3089751495 creator A5086358262 @default.
• W3089751495 creator A5087086583 @default.
• W3089751495 date "2020-10-03" @default.
• W3089751495 modified "2023-09-30" @default.
• W3089751495 title "Two independent approaches converge to the cloning of a new <i>Leptosphaeria maculans</i> avirulence effector gene, <i>AvrLmS-Lep2</i>" @default.
• W3089751495 cites W1832784998 @default.
• W3089751495 cites W1936727819 @default.
• W3089751495 cites W1974895740 @default.
• W3089751495 cites W1975779489 @default.
• W3089751495 cites W1982036537 @default.
• W3089751495 cites W1990801008 @default.
• W3089751495 cites W2001908713 @default.
• W3089751495 cites W2002769326 @default.
• W3089751495 cites W2015983397 @default.
• W3089751495 cites W2028743783 @default.
• W3089751495 cites W2029209906 @default.
• W3089751495 cites W2030832367 @default.
• W3089751495 cites W2031488725 @default.
• W3089751495 cites W2046007308 @default.
• W3089751495 cites W2053088447 @default.
• W3089751495 cites W2061366675 @default.
• W3089751495 cites W2072682718 @default.
• W3089751495 cites W2075857235 @default.
• W3089751495 cites W2081569915 @default.
• W3089751495 cites W2092615290 @default.
• W3089751495 cites W2094201044 @default.
• W3089751495 cites W2102619694 @default.
• W3089751495 cites W2108234281 @default.
• W3089751495 cites W2113280067 @default.
• W3089751495 cites W2117368344 @default.
• W3089751495 cites W2119180969 @default.
• W3089751495 cites W2120296258 @default.
• W3089751495 cites W2125937573 @default.
• W3089751495 cites W2131271579 @default.
• W3089751495 cites W2149992227 @default.
• W3089751495 cites W2173041129 @default.
• W3089751495 cites W2179421710 @default.
• W3089751495 cites W2207408507 @default.
• W3089751495 cites W2243973145 @default.
• W3089751495 cites W2415475022 @default.
• W3089751495 cites W2782568005 @default.
• W3089751495 cites W2800016513 @default.
• W3089751495 cites W2811101963 @default.
• W3089751495 cites W2900054628 @default.
• W3089751495 cites W2915376760 @default.
• W3089751495 cites W2942888469 @default.
• W3089751495 cites W2949850166 @default.
• W3089751495 cites W2952519878 @default.
• W3089751495 cites W2969779744 @default.
• W3089751495 cites W3034940604 @default.
• W3089751495 cites W4241389099 @default.
• W3089751495 cites W4366064192 @default.
• W3089751495 doi "https://doi.org/10.1101/2020.10.02.322479" @default.
• W3089751495 hasPublicationYear "2020" @default.
• W3089751495 type Work @default.
• W3089751495 sameAs 3089751495 @default.
• W3089751495 citedByCount "8" @default.
• W3089751495 countsByYear W30897514952020 @default.
• W3089751495 countsByYear W30897514952021 @default.
• W3089751495 countsByYear W30897514952022 @default.
• W3089751495 crossrefType "posted-content" @default.
• W3089751495 hasAuthorship W3089751495A5012907964 @default.
• W3089751495 hasAuthorship W3089751495A5013078077 @default.
• W3089751495 hasAuthorship W3089751495A5029142572 @default.
• W3089751495 hasAuthorship W3089751495A5040306269 @default.
• W3089751495 hasAuthorship W3089751495A5054738550 @default.
• W3089751495 hasAuthorship W3089751495A5056459986 @default.
• W3089751495 hasAuthorship W3089751495A5059081530 @default.
• W3089751495 hasAuthorship W3089751495A5064135644 @default.
• W3089751495 hasAuthorship W3089751495A5064481209 @default.
• W3089751495 hasAuthorship W3089751495A5068494489 @default.
• W3089751495 hasAuthorship W3089751495A5086358262 @default.
• W3089751495 hasAuthorship W3089751495A5087086583 @default.
• W3089751495 hasBestOaLocation W30897514951 @default.
• W3089751495 hasConcept C104317684 @default.
• W3089751495 hasConcept C121050878 @default.
• W3089751495 hasConcept C122735190 @default.
• W3089751495 hasConcept C127716648 @default.
• W3089751495 hasConcept C188082640 @default.
• W3089751495 hasConcept C199360897 @default.
• W3089751495 hasConcept C2776747608 @default.
• W3089751495 hasConcept C2777201196 @default.
• W3089751495 hasConcept C2779297677 @default.
• W3089751495 hasConcept C2779999212 @default.
• W3089751495 hasConcept C2780229518 @default.
• W3089751495 hasConcept C30481170 @default.
• W3089751495 hasConcept C41008148 @default.
• W3089751495 hasConcept C51785407 @default.

• next