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- W3089801963 abstract "RfaH is a compact two‐domain bacterial transcription factor that functions both as a regulator of transcription and an enhancer of translation. Underpinning the dual functional roles of RfaH is a partial but dramatic fold switch, which completely transforms the approximately 50‐amino acid C‐terminal domain (CTD) from an all‐α state to an all‐β state. The fold switch of the CTD occurs when RfaH binds to RNA polymerase (RNAP), however, the details of how this structural transformation is triggered is not well understood. Here we use all‐atom Monte Carlo simulations to characterize structural fluctuations and mechanical stability properties of the full‐length RfaH and the CTD as an isolated fragment. In agreement with experiments, we find that interdomain contacts are crucial for maintaining a stable, all‐α CTD in free RfaH. To probe mechanical properties, we use pulling simulations to measure the work required to inflict local deformations at different positions along the chain. The resulting mechanical stability profile reveals that free RfaH can be divided into a “rigid” part and a “soft” part, with a boundary that nearly coincides with the boundary between the two domains. We discuss the potential role of this feature for how fold switching may be triggered by interaction with RNAP. This article is protected by copyright. All rights reserved." @default.
- W3089801963 created "2020-10-08" @default.
- W3089801963 creator A5011206268 @default.
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- W3089801963 date "2020-10-10" @default.
- W3089801963 modified "2023-10-16" @default.
- W3089801963 title "Structural fluctuations and mechanical stabilities of the metamorphic protein <scp>RfaH</scp>" @default.
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- W3089801963 doi "https://doi.org/10.1002/prot.26014" @default.
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