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- W3089924408 abstract "The purpose of this study was to determine the characteristic and stability of Amniotic Membrane Stem Cell Metabolite Product (AMSC-MP) in fluid and freeze dried form. Conducted a qualitative test of the liquid and freeze dried AMSC-MP form using the SDSPAGE method, also determined the quantitative TGF-β levels, stability stored testing both materials at room and cold temperature during 28 days. Characterization of the freeze dried form was also carried out including FTIR profile, DTA, SEM and XRD.SDS-PAGE results obtained that qualitatively the liquid and freeze dried forms have the same protein component a molecule weight (MW) of 75.33 kDa. Quantitatively, the form of fluid has higher TGF-β levels compared to freeze dried on day 0. However, the results of the stability test showed that the form of freeze dried has better stability than the fluid, as indicated by a decrease in TGF-β levels greater on the day 21st. Liquid form that is stored at room temperature changes the color after 7days. Furthermore characterization of freeze dried shows has a crystalline structure based on its XRD profile with an endothermic peak at 163.8 this is supported by SEM a tetragonal crystal. FTIR profile showed the maximum absorption at wave numbers 1674-1640 and 3350-3200 which indicates the presence of C=O and N-H bonds which are functional groups of protein compounds.Conclusion: The freeze dried AMSC-MP form has better stability than the fluid form in cold temperature storage and AMSC-MP is a protein." @default.
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- W3089924408 date "2020-02-01" @default.
- W3089924408 modified "2023-10-14" @default.
- W3089924408 title "Characterization and Stability Study of Amniotic Membrane Stem Cell Metabolite Product (AMSC-MP)" @default.
- W3089924408 doi "https://doi.org/10.21276/ijprhs.2020.01.02" @default.
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