Matches in SemOpenAlex for { <https://semopenalex.org/work/W3090231781> ?p ?o ?g. }
- W3090231781 endingPage "112683" @default.
- W3090231781 startingPage "112683" @default.
- W3090231781 abstract "Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) for the analysis of zearalenone (ZEA), one of the most frequently encountered mycotoxins in food worldwide. Instead of a toxin-conjugate conventionally used in competitive immunoassays, we designed a ZEA mimicking peptide extended by a biotin-linker and confirmed its excellent suitability to mimic ZEA by nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analysis. Upconversion nanoparticles (UCNP, type NaYF4:Yb,Tm) served as background-free optical label for the detection of the peptide mimetic in the competitive ULISA. Streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. The UCNP conjugate clearly outperformed conventional labels such as enzymes or fluorescent dyes. With a limit of detection of 20 pg mL−1 (63 pM), the competitive ULISA is well applicable to the detection of ZEA at the levels set by the European legislation. Moreover, the ULISA is specific for ZEA and its metabolites (α- and β-zearalenol) without significant cross-reactivity with other related mycotoxins. We detected ZEA in spiked and naturally contaminated maize samples using liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) as a reference method to demonstrate food analysis in real samples." @default.
- W3090231781 created "2020-10-08" @default.
- W3090231781 creator A5000597135 @default.
- W3090231781 creator A5007030984 @default.
- W3090231781 creator A5009961097 @default.
- W3090231781 creator A5011789826 @default.
- W3090231781 creator A5018410091 @default.
- W3090231781 creator A5029429628 @default.
- W3090231781 creator A5039015701 @default.
- W3090231781 creator A5052164855 @default.
- W3090231781 creator A5054938337 @default.
- W3090231781 creator A5056527826 @default.
- W3090231781 creator A5079363307 @default.
- W3090231781 creator A5082301783 @default.
- W3090231781 date "2020-12-01" @default.
- W3090231781 modified "2023-10-07" @default.
- W3090231781 title "Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone" @default.
- W3090231781 cites W1544971129 @default.
- W3090231781 cites W1668990467 @default.
- W3090231781 cites W1733568468 @default.
- W3090231781 cites W1996336187 @default.
- W3090231781 cites W1999441504 @default.
- W3090231781 cites W2010774103 @default.
- W3090231781 cites W2030791074 @default.
- W3090231781 cites W2075682176 @default.
- W3090231781 cites W2084586277 @default.
- W3090231781 cites W2095030337 @default.
- W3090231781 cites W2127126181 @default.
- W3090231781 cites W2139574393 @default.
- W3090231781 cites W2147203421 @default.
- W3090231781 cites W2219228819 @default.
- W3090231781 cites W2225292601 @default.
- W3090231781 cites W2288948102 @default.
- W3090231781 cites W2322536997 @default.
- W3090231781 cites W2329179116 @default.
- W3090231781 cites W2405057777 @default.
- W3090231781 cites W2568192871 @default.
- W3090231781 cites W2610509862 @default.
- W3090231781 cites W2739579980 @default.
- W3090231781 cites W2767823092 @default.
- W3090231781 cites W2767966695 @default.
- W3090231781 cites W2797886716 @default.
- W3090231781 cites W2886033880 @default.
- W3090231781 cites W2893003169 @default.
- W3090231781 cites W2894999772 @default.
- W3090231781 cites W2896923286 @default.
- W3090231781 cites W2919830774 @default.
- W3090231781 cites W2934860357 @default.
- W3090231781 cites W2936426664 @default.
- W3090231781 cites W2955144726 @default.
- W3090231781 cites W2968214861 @default.
- W3090231781 cites W2984825384 @default.
- W3090231781 cites W2988395938 @default.
- W3090231781 cites W2996037751 @default.
- W3090231781 cites W3004932152 @default.
- W3090231781 cites W3011529694 @default.
- W3090231781 cites W3011572515 @default.
- W3090231781 cites W3014124124 @default.
- W3090231781 cites W3015038901 @default.
- W3090231781 cites W3015972573 @default.
- W3090231781 cites W3043635829 @default.
- W3090231781 cites W3083631702 @default.
- W3090231781 cites W4211210890 @default.
- W3090231781 cites W4211237594 @default.
- W3090231781 doi "https://doi.org/10.1016/j.bios.2020.112683" @default.
- W3090231781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33069054" @default.
- W3090231781 hasPublicationYear "2020" @default.
- W3090231781 type Work @default.
- W3090231781 sameAs 3090231781 @default.
- W3090231781 citedByCount "33" @default.
- W3090231781 countsByYear W30902317812021 @default.
- W3090231781 countsByYear W30902317812022 @default.
- W3090231781 countsByYear W30902317812023 @default.
- W3090231781 crossrefType "journal-article" @default.
- W3090231781 hasAuthorship W3090231781A5000597135 @default.
- W3090231781 hasAuthorship W3090231781A5007030984 @default.
- W3090231781 hasAuthorship W3090231781A5009961097 @default.
- W3090231781 hasAuthorship W3090231781A5011789826 @default.
- W3090231781 hasAuthorship W3090231781A5018410091 @default.
- W3090231781 hasAuthorship W3090231781A5029429628 @default.
- W3090231781 hasAuthorship W3090231781A5039015701 @default.
- W3090231781 hasAuthorship W3090231781A5052164855 @default.
- W3090231781 hasAuthorship W3090231781A5054938337 @default.
- W3090231781 hasAuthorship W3090231781A5056527826 @default.
- W3090231781 hasAuthorship W3090231781A5079363307 @default.
- W3090231781 hasAuthorship W3090231781A5082301783 @default.
- W3090231781 hasConcept C106847996 @default.
- W3090231781 hasConcept C119128265 @default.
- W3090231781 hasConcept C134306372 @default.
- W3090231781 hasConcept C155672457 @default.
- W3090231781 hasConcept C159654299 @default.
- W3090231781 hasConcept C171250308 @default.
- W3090231781 hasConcept C185592680 @default.
- W3090231781 hasConcept C192562407 @default.
- W3090231781 hasConcept C197336794 @default.
- W3090231781 hasConcept C203014093 @default.
- W3090231781 hasConcept C2776008721 @default.
- W3090231781 hasConcept C2776190903 @default.