Matches in SemOpenAlex for { <https://semopenalex.org/work/W3090402362> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W3090402362 abstract "To determine genotype frequency of biallelic single nucleotide polymorphisms and its use in detection of informative allele in donor/recipient pairs (sibling pairs) having undergone haematopoietic stem cell transplantation with various haematological disorders using a PCR based method.This descriptive study was conducted at GRC Lab Rawalpindi from Jan 2018- Oct 2019.A total of twenty donor/ recipient pairs (sibling pairs) were studied for genotype frequency and informativeness of single nucleotide polymorphisms. Genomic DNA was extracted from the peripheral blood and amplification of single nucleotide polymorphisms was done by PCR based method. The amplified DNA was seen by electrophoresis on 6% polyacrylamide gel.A sharp band of DNA on the polyacrylamide gel indicated a positive reaction. At least two or more informative SNP markers were found in every sibling pair.Our results demonstrate that PCR amplification of polyacrylamide gel electrophoresis using single nucleotide polymorphism has allowed the successful screening and detection of informative allele in all the donor/recipient pairs. (Sibling pairs). This PCR based assay using SNPs appears to be a quick, simple, reliable and technically feasible method for a use in a Pakistani setting." @default.
- W3090402362 created "2020-10-08" @default.
- W3090402362 creator A5058491280 @default.
- W3090402362 creator A5070398672 @default.
- W3090402362 date "2020-09-28" @default.
- W3090402362 modified "2023-10-17" @default.
- W3090402362 title "Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction" @default.
- W3090402362 cites W1980066896 @default.
- W3090402362 cites W2006714444 @default.
- W3090402362 cites W2011208086 @default.
- W3090402362 cites W2028407627 @default.
- W3090402362 cites W2050680849 @default.
- W3090402362 cites W2061008984 @default.
- W3090402362 cites W2063299523 @default.
- W3090402362 cites W2081608868 @default.
- W3090402362 cites W2107916366 @default.
- W3090402362 cites W2129973156 @default.
- W3090402362 cites W2137393500 @default.
- W3090402362 doi "https://doi.org/10.12669/pjms.36.7.2998" @default.
- W3090402362 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7674877" @default.
- W3090402362 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33235576" @default.
- W3090402362 hasPublicationYear "2020" @default.
- W3090402362 type Work @default.
- W3090402362 sameAs 3090402362 @default.
- W3090402362 citedByCount "1" @default.
- W3090402362 countsByYear W30904023622022 @default.
- W3090402362 crossrefType "journal-article" @default.
- W3090402362 hasAuthorship W3090402362A5058491280 @default.
- W3090402362 hasAuthorship W3090402362A5070398672 @default.
- W3090402362 hasBestOaLocation W30904023621 @default.
- W3090402362 hasConcept C104317684 @default.
- W3090402362 hasConcept C135763542 @default.
- W3090402362 hasConcept C153209595 @default.
- W3090402362 hasConcept C153911025 @default.
- W3090402362 hasConcept C17757408 @default.
- W3090402362 hasConcept C180754005 @default.
- W3090402362 hasConcept C37463918 @default.
- W3090402362 hasConcept C49105822 @default.
- W3090402362 hasConcept C54355233 @default.
- W3090402362 hasConcept C552990157 @default.
- W3090402362 hasConcept C71924100 @default.
- W3090402362 hasConcept C86803240 @default.
- W3090402362 hasConceptScore W3090402362C104317684 @default.
- W3090402362 hasConceptScore W3090402362C135763542 @default.
- W3090402362 hasConceptScore W3090402362C153209595 @default.
- W3090402362 hasConceptScore W3090402362C153911025 @default.
- W3090402362 hasConceptScore W3090402362C17757408 @default.
- W3090402362 hasConceptScore W3090402362C180754005 @default.
- W3090402362 hasConceptScore W3090402362C37463918 @default.
- W3090402362 hasConceptScore W3090402362C49105822 @default.
- W3090402362 hasConceptScore W3090402362C54355233 @default.
- W3090402362 hasConceptScore W3090402362C552990157 @default.
- W3090402362 hasConceptScore W3090402362C71924100 @default.
- W3090402362 hasConceptScore W3090402362C86803240 @default.
- W3090402362 hasLocation W30904023621 @default.
- W3090402362 hasLocation W30904023622 @default.
- W3090402362 hasLocation W30904023623 @default.
- W3090402362 hasOpenAccess W3090402362 @default.
- W3090402362 hasPrimaryLocation W30904023621 @default.
- W3090402362 hasRelatedWork W11953848 @default.
- W3090402362 hasRelatedWork W12478100 @default.
- W3090402362 hasRelatedWork W13097937 @default.
- W3090402362 hasRelatedWork W13726115 @default.
- W3090402362 hasRelatedWork W16693249 @default.
- W3090402362 hasRelatedWork W17396074 @default.
- W3090402362 hasRelatedWork W19151415 @default.
- W3090402362 hasRelatedWork W6347863 @default.
- W3090402362 hasRelatedWork W7032323 @default.
- W3090402362 hasRelatedWork W9209984 @default.
- W3090402362 isParatext "false" @default.
- W3090402362 isRetracted "false" @default.
- W3090402362 magId "3090402362" @default.
- W3090402362 workType "article" @default.