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• W3090776618 endingPage "1734" @default.
• W3090776618 startingPage "1724" @default.
• W3090776618 abstract "Although transport of molecules into cells via electroporation is a common biomedical procedure, its protocols are often based on trial and error. Despite a long history of theoretical effort, the underlying mechanisms of cell membrane electroporation are not sufficiently elucidated, in part, because of the number of independent fitting parameters needed to link theory to experiment. Here, we ask if the electroporation behavior of a reduced cell membrane is consistent with time-resolved, atomistic, molecular dynamics (MD) simulations of phospholipid bilayers responding to electric fields. To avoid solvent and tension effects, giant unilamellar vesicles (GUVs) were used, and transport kinetics were measured by the entry of the impermeant fluorescent dye calcein. Because the timescale of electrical pulses needed to restructure bilayers into pores is much shorter than the time resolution of current techniques for membrane transport kinetics measurements, the lifetimes of lipid bilayer electropores were measured using systematic variation of the initial MD simulation conditions, whereas GUV transport kinetics were detected in response to a nanosecond timescale variation in the applied electric pulse lifetimes and interpulse intervals. Molecular transport after GUV permeabilization induced by multiple pulses is additive for interpulse intervals as short as 50 ns but not 5-ns intervals, consistent with the 10-50-ns lifetimes of electropores in MD simulations. Although the results were mostly consistent between GUV and MD simulations, the kinetics of ultrashort, electric-field-induced permeabilization of GUVs were significantly different from published results in cells exposed to ultrashort (6 and 2 ns) electric fields, suggesting that cellular electroporation involves additional structures and processes." @default.
• W3090776618 created "2020-10-08" @default.
• W3090776618 creator A5006870070 @default.
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• W3090776618 date "2020-11-01" @default.
• W3090776618 modified "2023-10-17" @default.
• W3090776618 title "Dye Transport through Bilayers Agrees with Lipid Electropore Molecular Dynamics" @default.
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• W3090776618 cites W1967905520 @default.
• W3090776618 cites W1969959009 @default.
• W3090776618 cites W1973142530 @default.
• W3090776618 cites W1976499671 @default.
• W3090776618 cites W1979328135 @default.
• W3090776618 cites W2004620568 @default.
• W3090776618 cites W2008800998 @default.
• W3090776618 cites W2014061733 @default.
• W3090776618 cites W2014567501 @default.
• W3090776618 cites W2021520922 @default.
• W3090776618 cites W2022603060 @default.
• W3090776618 cites W2022803930 @default.
• W3090776618 cites W2033495141 @default.
• W3090776618 cites W2035266068 @default.
• W3090776618 cites W2035687084 @default.
• W3090776618 cites W2037581262 @default.
• W3090776618 cites W2045558911 @default.
• W3090776618 cites W2051960501 @default.
• W3090776618 cites W2054853170 @default.
• W3090776618 cites W2057477511 @default.
• W3090776618 cites W2057777676 @default.
• W3090776618 cites W2066414494 @default.
• W3090776618 cites W2069041223 @default.
• W3090776618 cites W2070310244 @default.
• W3090776618 cites W2071624468 @default.
• W3090776618 cites W2071747332 @default.
• W3090776618 cites W2073162912 @default.
• W3090776618 cites W2080271268 @default.
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• W3090776618 cites W2092575669 @default.
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• W3090776618 cites W2101898293 @default.
• W3090776618 cites W2103245465 @default.
• W3090776618 cites W2103945336 @default.
• W3090776618 cites W2117149845 @default.
• W3090776618 cites W2121381406 @default.
• W3090776618 cites W2122590911 @default.
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• W3090776618 cites W2155161956 @default.
• W3090776618 cites W2155703402 @default.
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• W3090776618 cites W2316206799 @default.
• W3090776618 cites W2321968234 @default.
• W3090776618 cites W2330442656 @default.
• W3090776618 cites W2342739775 @default.
• W3090776618 cites W2512379437 @default.
• W3090776618 cites W2586222320 @default.
• W3090776618 cites W2592576464 @default.
• W3090776618 cites W2608448109 @default.
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• W3090776618 doi "https://doi.org/10.1016/j.bpj.2020.09.028" @default.
• W3090776618 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7677249" @default.
• W3090776618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33096018" @default.
• W3090776618 hasPublicationYear "2020" @default.
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