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• W3090840260 abstract "Retroviral insertional mutagenesis (RIM) is both a relevant risk in gene therapy and a powerful tool for identifying genes that enhance the competitiveness of repopulating hematopoietic stem and progenitor cells (HSPCs). However, focusing only on the gene closest to the retroviral vector insertion site (RVIS) may underestimate the effects of RIM, as dysregulation of distal and/or multiple genes by a single insertion event was reported in several studies. As a proof of concept, we examined the common insertion site (CIS) Bcl-xL, which revealed seven genes located within ±150 kb from the RVIS for our study. We confirmed that Bcl-xL enhanced the competitiveness of HSPCs, whereas the Bcl-xL neighbor Id1 hindered HSPC long-term repopulation. This negative influence of Id1 could be counteracted by co-expressing Bcl-xL. Interestingly, >90% of early reconstituted myeloid cells were found to originate from transduced HSPCs upon simultaneous overexpression of Bcl-xL and Id1, which implies that Bcl-xL and Id1 can collaborate to rapidly replenish the myeloid compartment under stress conditions. To directly compare the competitiveness of HSPCs conveyed by multiple transgenes, we developed a multiple competitor competitive repopulation (MCCR) assay to simultaneously screen effects on HSPC repopulating capacity in a single mouse. The MCCR assay revealed that multiple genes within a CIS can have positive or negative impact on hematopoiesis. Furthermore, these data highlight the importance of studying multiple genes located within the proximity of an insertion site to understand complex biological effects, especially as the number of gene therapy patients increases." @default.
• W3090840260 created "2020-10-08" @default.
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• W3090840260 date "2021-05-01" @default.
• W3090840260 modified "2023-10-14" @default.
• W3090840260 title "Multiple Genes Surrounding <i>Bcl-x_L</i>, a Common Retroviral Insertion Site, Can Influence Hematopoiesis Individually or in Concert" @default.
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• W3090840260 cites W1966530560 @default.
• W3090840260 cites W1966774802 @default.
• W3090840260 cites W1966790428 @default.
• W3090840260 cites W1967350125 @default.
• W3090840260 cites W1968561263 @default.
• W3090840260 cites W1971062917 @default.
• W3090840260 cites W1971946672 @default.
• W3090840260 cites W1976246831 @default.
• W3090840260 cites W1976953797 @default.
• W3090840260 cites W1977287641 @default.
• W3090840260 cites W1985991486 @default.
• W3090840260 cites W1992900585 @default.
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• W3090840260 cites W2003934533 @default.
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• W3090840260 cites W2027838649 @default.
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• W3090840260 cites W2063254961 @default.
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• W3090840260 cites W2068972073 @default.
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• W3090840260 cites W2077338924 @default.
• W3090840260 cites W2078149732 @default.
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• W3090840260 doi "https://doi.org/10.1089/hum.2019.344" @default.
• W3090840260 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33012194" @default.
• W3090840260 hasPublicationYear "2021" @default.
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