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- W3091270345 abstract "Abstract Introduction: MicroRNAs play a critical role in EMT regulation through tightly regulating the transcription factors. EMT (Epithelial-Mesenchymal Transition) in the colorectal cancer cell (CRC) is a highly controlled mechanism, contributing to the development of progressive cancer. Throughout this research, miR-200a/205 was focused as a component implicated throughout regulating the EMT process in colorectal cancer cells. In this sense, the induction of the EMT process was made using colorectal cancer cell lines.Materials and Methods: The mRNA levels of E-cadherin, Vimentin, β-catenin, Zeb1, and Snail were determined using real time-PCR for characterization of the EMT process. True real time-PCR was conducted for evaluating the alteration amount of microRNAs.Results: The findings of this study verified the in-vitro EMT model being developed. The in vitro analysis revealed a negative correlation between the Zeb1 and Snail miR-200a and miR205 (P=0.001) (P=0.0001). The results of miR-200a and miR205 are regulated down in vitro.Conclusion: miR-200a and miR205 may be used as candidates in further research to prevent colorectal cancer's invasive properties via the EMT process." @default.
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- W3091270345 date "2020-09-26" @default.
- W3091270345 modified "2023-09-23" @default.
- W3091270345 title "Association of miR-200a and miR-205 Expression with Epithelial-Mesenchymal Transition in Colorectal Cancer Cells" @default.
- W3091270345 doi "https://doi.org/10.22037/aab.v11i3.31476" @default.
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