FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3091640303> ?p ?o ?g. }

• W3091640303 endingPage "1022" @default.
• W3091640303 startingPage "1008" @default.
• W3091640303 abstract "Mast cells (MCs) are pro-inflammatory tissue-resident immune cells that play a key role in inflammation. MCs circulate in peripheral blood as progenitors and undergo terminal differentiation in the tissue microenvironment where they can remain for many years. This in situ maturation results in tissue- and species-specific MC phenotypes, culminating in significant variability in response to environmental stimuli. There are many challenges associated with studying mature tissue-derived MCs, particularly in humans. In cases where cultured MCs are able to differentiate in two-dimensional in vitro cultures, there remains an inability for full maturation. Extracellular matrix (ECM) scaffolds provide for a more physiologically relevant environment for cells in vitro and have been shown to modulate the response of other immune cells such as T cells, monocytes, and macrophages. To improve current in vitro testing platforms of MCs and to assess future use of ECM scaffolds for MC regulation, we studied the in vitro response of human MCs cultured on decellularized porcine dermis hydrogels (dermis extracellular matrix hydrogel [dECM-H]). This study investigated the effect of dECM-H on cellular metabolic activity, cell viability, and receptor expression compared to collagen type I hydrogel (Collagen-H). Human MCs showed different metabolic activity when cultured in the dECM-H and also upregulated immunoglobulin E (IgE) receptors associated with MC maturation/activation compared to collagen type I. These results suggest an overall benefit in the long-term culture of human MCs in the dECM-H compared to Collagen-H providing important steps toward a model that is more representative of in vivo conditions. Mast cells (MCs) are difficult to culture in vitro as current culture conditions and substrates fail to promote similar phenotypic features observed in vivo. Extracellular matrix (ECM)-based biomaterials offer three-dimensional, tissue-specific environments that more closely resemble in vivo conditions. Our study explores the use of dermal ECM hydrogels for MC culture and shows significant upregulation of metabolic activity, cell viability, and gene expression of markers associated with MC maturation or activation compared to collagen type I-hydrogel and tissue culture plastic controls at 7 days. These results are among the first to describe MC behavior in response to ECM hydrogels." @default.
• W3091640303 created "2020-10-08" @default.
• W3091640303 creator A5000775960 @default.
• W3091640303 creator A5002379615 @default.
• W3091640303 creator A5009940383 @default.
• W3091640303 creator A5019847998 @default.
• W3091640303 creator A5023277450 @default.
• W3091640303 creator A5047025517 @default.
• W3091640303 creator A5074952330 @default.
• W3091640303 creator A5083291686 @default.
• W3091640303 date "2021-08-01" @default.
• W3091640303 modified "2023-10-02" @default.
• W3091640303 title "Dermal Extracellular Matrix-Derived Hydrogels as an <i>In Vitro</i> Substrate to Study Mast Cell Maturation" @default.
• W3091640303 cites W102174519 @default.
• W3091640303 cites W146090589 @default.
• W3091640303 cites W1497757154 @default.
• W3091640303 cites W1505264719 @default.
• W3091640303 cites W1509033214 @default.
• W3091640303 cites W1539863156 @default.
• W3091640303 cites W1595249201 @default.
• W3091640303 cites W1606413143 @default.
• W3091640303 cites W1845412871 @default.
• W3091640303 cites W1862989971 @default.
• W3091640303 cites W1890077533 @default.
• W3091640303 cites W1951686243 @default.
• W3091640303 cites W1973957435 @default.
• W3091640303 cites W1975342014 @default.
• W3091640303 cites W1979462929 @default.
• W3091640303 cites W1987602789 @default.
• W3091640303 cites W1989036737 @default.
• W3091640303 cites W1991027120 @default.
• W3091640303 cites W2005200593 @default.
• W3091640303 cites W2006580685 @default.
• W3091640303 cites W2011590786 @default.
• W3091640303 cites W2013635497 @default.
• W3091640303 cites W2016523415 @default.
• W3091640303 cites W2017335231 @default.
• W3091640303 cites W2021557819 @default.
• W3091640303 cites W2022849203 @default.
• W3091640303 cites W2023167946 @default.
• W3091640303 cites W2035073334 @default.
• W3091640303 cites W2038545269 @default.
• W3091640303 cites W2039112412 @default.
• W3091640303 cites W2042022714 @default.
• W3091640303 cites W2044504507 @default.
• W3091640303 cites W2048979058 @default.
• W3091640303 cites W2049082059 @default.
• W3091640303 cites W2054635074 @default.
• W3091640303 cites W2057121443 @default.
• W3091640303 cites W2059486645 @default.
• W3091640303 cites W2059910471 @default.
• W3091640303 cites W2064180188 @default.
• W3091640303 cites W2073365757 @default.
• W3091640303 cites W2074419825 @default.
• W3091640303 cites W2075748521 @default.
• W3091640303 cites W2078561828 @default.
• W3091640303 cites W2080719665 @default.
• W3091640303 cites W2086862049 @default.
• W3091640303 cites W2086965338 @default.
• W3091640303 cites W2098044666 @default.
• W3091640303 cites W2106099967 @default.
• W3091640303 cites W2109125534 @default.
• W3091640303 cites W2114305100 @default.
• W3091640303 cites W2114570899 @default.
• W3091640303 cites W2119256134 @default.
• W3091640303 cites W2123556483 @default.
• W3091640303 cites W2128308244 @default.
• W3091640303 cites W2137828907 @default.
• W3091640303 cites W2154936272 @default.
• W3091640303 cites W2163435138 @default.
• W3091640303 cites W2169075370 @default.
• W3091640303 cites W2179695532 @default.
• W3091640303 cites W2225150806 @default.
• W3091640303 cites W2287025129 @default.
• W3091640303 cites W2336642884 @default.
• W3091640303 cites W2347163170 @default.
• W3091640303 cites W2411765633 @default.
• W3091640303 cites W2413734684 @default.
• W3091640303 cites W2520858311 @default.
• W3091640303 cites W2574998181 @default.
• W3091640303 cites W2586499858 @default.
• W3091640303 cites W2592704774 @default.
• W3091640303 cites W2737628422 @default.
• W3091640303 cites W2737984947 @default.
• W3091640303 cites W2748957178 @default.
• W3091640303 cites W2748969123 @default.
• W3091640303 cites W2768599619 @default.
• W3091640303 cites W2800548936 @default.
• W3091640303 cites W2837928171 @default.
• W3091640303 cites W2839646944 @default.
• W3091640303 cites W2900213480 @default.
• W3091640303 cites W2925333081 @default.
• W3091640303 cites W2945658966 @default.
• W3091640303 cites W2952800810 @default.
• W3091640303 cites W2981167732 @default.
• W3091640303 cites W3002971935 @default.
• W3091640303 cites W3034458203 @default.
• W3091640303 cites W4251892294 @default.

• next