FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3091755319> ?p ?o ?g. }

• W3091755319 abstract "Abstract To date sperm-oviduct interactions have largely been investigated under in vitro conditions. Therefore we set out to characterize the behaviour of bovine spermatozoa within the sperm reservoir under near in vivo conditions and in real-time using a novel live cell imaging technology and a newly established fluorescent sperm binding assay. Sperm structure and tubal reactions after sperm binding were analysed using scanning and transmission electron microscopy and histochemistry. As a model to specify the impact of stress on sperm-oviduct interactions, frozen-thawed conventional and sex-sorted spermatozoa from the same bulls (n = 7) were co-incubated with oviducts obtained from cows immediately after slaughter. Our studies revealed that within the oviductal sperm reservoir agile (bound at a tangential angle of about 30°, actively beating undulating tail), lagging (bound at a lower angle, reduced tail movement), immotile (absence of tail movement) and hyperactivated (whip-like movement of tail) spermatozoa occur, the prevalence of which changes in a time-dependent pattern. After formation of the sperm reservoir, tubal ciliary beat frequency is significantly increased ( p = 0.022) and the epithelial cells show increased activity of endoplasmic reticula. After sex sorting, spermatozoa occasionally display abnormal movement patterns characterized by a 360° rotating head and tail. Sperm binding in the oviduct is significantly reduced ( p = 0.008) following sexing. Sex-sorted spermatozoa reveal deformations in the head, sharp bends in the tail and a significantly increased prevalence of damaged mitochondria ( p < 0.001). Our results imply that the oviductal cells specifically react to the binding of spermatozoa, maintaining sperm survival within the tubal reservoir. The sex-sorting process, which is associated with mechanical, chemical and time stress, impacts sperm binding to the oviduct and mitochondrial integrity affecting sperm motility and function." @default.
• W3091755319 created "2020-10-08" @default.
• W3091755319 creator A5000531406 @default.
• W3091755319 creator A5017769481 @default.
• W3091755319 creator A5045312273 @default.
• W3091755319 creator A5053319200 @default.
• W3091755319 date "2020-10-05" @default.
• W3091755319 modified "2023-10-02" @default.
• W3091755319 title "Bovine sperm-oviduct interactions are characterized by specific sperm behaviour, ultrastructure and tubal reactions which are impacted by sex sorting" @default.
• W3091755319 cites W133447721 @default.
• W3091755319 cites W1854046621 @default.
• W3091755319 cites W1965273370 @default.
• W3091755319 cites W1980241059 @default.
• W3091755319 cites W1994919749 @default.
• W3091755319 cites W1998752745 @default.
• W3091755319 cites W2008065225 @default.
• W3091755319 cites W2009092555 @default.
• W3091755319 cites W2009986576 @default.
• W3091755319 cites W2012177408 @default.
• W3091755319 cites W2015550884 @default.
• W3091755319 cites W2016722679 @default.
• W3091755319 cites W2016843994 @default.
• W3091755319 cites W2022864664 @default.
• W3091755319 cites W2039698632 @default.
• W3091755319 cites W2044242150 @default.
• W3091755319 cites W2050615109 @default.
• W3091755319 cites W2053400113 @default.
• W3091755319 cites W2053786989 @default.
• W3091755319 cites W2055450941 @default.
• W3091755319 cites W2055837145 @default.
• W3091755319 cites W2070922502 @default.
• W3091755319 cites W2075148921 @default.
• W3091755319 cites W2078231188 @default.
• W3091755319 cites W2079624291 @default.
• W3091755319 cites W2082521446 @default.
• W3091755319 cites W2088903948 @default.
• W3091755319 cites W2092052252 @default.
• W3091755319 cites W2093245707 @default.
• W3091755319 cites W2094463280 @default.
• W3091755319 cites W2095232474 @default.
• W3091755319 cites W2097098722 @default.
• W3091755319 cites W2102525950 @default.
• W3091755319 cites W2105155857 @default.
• W3091755319 cites W2107652646 @default.
• W3091755319 cites W2109624880 @default.
• W3091755319 cites W2112878750 @default.
• W3091755319 cites W2114923918 @default.
• W3091755319 cites W2116388049 @default.
• W3091755319 cites W2118940704 @default.
• W3091755319 cites W2120014701 @default.
• W3091755319 cites W2123832135 @default.
• W3091755319 cites W2128688025 @default.
• W3091755319 cites W2133658410 @default.
• W3091755319 cites W2134233197 @default.
• W3091755319 cites W2134610096 @default.
• W3091755319 cites W2137098270 @default.
• W3091755319 cites W2137873625 @default.
• W3091755319 cites W2144870423 @default.
• W3091755319 cites W2145701451 @default.
• W3091755319 cites W2147227113 @default.
• W3091755319 cites W2148069201 @default.
• W3091755319 cites W2148448950 @default.
• W3091755319 cites W2150566463 @default.
• W3091755319 cites W2150638407 @default.
• W3091755319 cites W2151595874 @default.
• W3091755319 cites W2153344238 @default.
• W3091755319 cites W2155948635 @default.
• W3091755319 cites W2157648764 @default.
• W3091755319 cites W2162660587 @default.
• W3091755319 cites W2165064047 @default.
• W3091755319 cites W2166372453 @default.
• W3091755319 cites W2261290973 @default.
• W3091755319 cites W2282600098 @default.
• W3091755319 cites W2339396927 @default.
• W3091755319 cites W2341422510 @default.
• W3091755319 cites W2396805552 @default.
• W3091755319 cites W2400089061 @default.
• W3091755319 cites W2404394342 @default.
• W3091755319 cites W2510813896 @default.
• W3091755319 cites W2511130026 @default.
• W3091755319 cites W2758706445 @default.
• W3091755319 cites W2767459494 @default.
• W3091755319 cites W2769025277 @default.
• W3091755319 cites W2785433598 @default.
• W3091755319 cites W2791908562 @default.
• W3091755319 cites W2792416671 @default.
• W3091755319 cites W2792574914 @default.
• W3091755319 cites W2794012893 @default.
• W3091755319 cites W2806016388 @default.
• W3091755319 cites W2809196778 @default.
• W3091755319 cites W2899847813 @default.
• W3091755319 cites W2899917148 @default.
• W3091755319 cites W2937301376 @default.
• W3091755319 cites W2963561003 @default.
• W3091755319 cites W2969666390 @default.
• W3091755319 cites W2996076422 @default.
• W3091755319 cites W3014054790 @default.
• W3091755319 cites W3016148414 @default.
• W3091755319 cites W3102273162 @default.
• W3091755319 cites W82918339 @default.

• next