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- W3091805183 abstract "HomePlant DiseaseVol. 105, No. 3First Report of Botryosphaeria dothidea Causing Stem Canker and Dieback of Gleditsia sinensis in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Botryosphaeria dothidea Causing Stem Canker and Dieback of Gleditsia sinensis in ChinaFeiyan Huang, Min Yang, Jiani Liu, Zebin Chen, Yu Fang, Qiyu Wang, Shifeng He, Yu Zhong, and Lei YuFeiyan Huanghttp://orcid.org/0000-0002-5166-0848College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this author, Min YangCollege of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this author, Jiani Liu†Corresponding authors: J. N. Liu; E-mail Address: 47067521@qq.com and L. Yu; E-mail Address: yulei0425@163.comCollege of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this author, Zebin ChenCollege of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this author, Yu FangCollege of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this author, Qiyu WangCollege of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this author, Shifeng HeCollege of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this author, Yu ZhongCollege of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this author, and Lei Yu†Corresponding authors: J. N. Liu; E-mail Address: 47067521@qq.com and L. Yu; E-mail Address: yulei0425@163.comhttp://orcid.org/0000-0001-6638-9803College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, ChinaSearch for more papers by this authorAffiliationsAuthors and Affiliations Feiyan Huang Min Yang Jiani Liu † Zebin Chen Yu Fang Qiyu Wang Shifeng He Yu Zhong Lei Yu † College of Agronomy, Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, China Published Online:25 Jan 2021https://doi.org/10.1094/PDIS-05-20-1074-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleGleditsia sinensis Lam is a kind of legume perennial woody plant, which is a traditional Chinese medicine with high economic and ecological value. Its planting area is about 0.1 million hectares. In July 2018, symptoms of stem blight were observed on G. sinensis in An shun (26.072311°N, 106.097433°E), Guizhou province (southwestern China). Symptoms included stem canker and dieback, twig blight, and extensive vascular discoloration, with incidence rate of 45 to 70%. Samples from plants with symptoms were washed with running tap water, surface sterilized with 2% sodium hypochlorite and then 75% ethanol, rinsed in sterile distilled water, plated on potato dextrose agar (PDA), and incubated at 28°C. Fungal isolates developed copious white aerial mycelium that became dark gray after 4 to 5 days and formed black pycnidia after 23 days. Single-hyphal-tip cultures of 27 putative isolates were stored in the culture collection (CMW) of the Urban Modern Agriculture Engineering Research Center at the Kunming University. Conidia forming on pycnidia were one-celled, hyaline, aseptate, and fusiform, with dimensions of 20.3 to 25.9 × 4.2 to 6.5 μm (average 22.5 × 5.5 μm) (60 conidia were measured). DNA sequence analysis of the ribosomal internal transcribed spacer regions ITS1-5.8S-ITS4, β-tubulin, and the translation elongation factor 1-alpha (EF1-α) were performed. BLAST searches at GenBank showed the highest nucleotide sequence identity with Botryosphaeria dothidea reference sequence (ITS: >99.82%, KR708996; EF1-α: 99.62%, KP183214; β-tubulin: >99.67%, KU306116). Representative sequences of isolates from these regions were deposited in GenBank (ITS: MT449017; EF1-α: MT454342; β-tubulin: MT454343). Morphological and molecular results confirmed this species as B. dothidea (Aguirre et al. 2018). Pathogenicity tests were conducted by stem inoculation of 2-year-old G. sinensis seedlings. Mycelial plugs (2 to 3 mm in diameter) from actively growing colonies of B. dothidea (PDA) were applied to same-size bark wounds on the middle point of the stems. Inoculated wounds were wrapped with Parafilm. Control seedlings received sterile PDA plugs. Inoculated and control seedlings (nine each) were kept in the greenhouse (28°C, 80% humidity). After 10 days, all of the inoculated plants showed dark vascular stem tissue, and the controls remained healthy. After 30 days, all of the inoculated but none of the control G. sinensis seedlings showed leaf wilting and tissue discoloration. B. dothidea was reisolated from symptomatic tissues, thus fulfilling Koch’s postulates. No symptoms were visible in the control seedlings, and no B. dothidea was reisolated from the control seedling tissues. B. dothidea is a member of Botryosphaeriaceae, commonly associated with cankers and dieback of woody plants. B. dothidea has been reported as a pathogen causing stem dieback and branch canker on Malosma laurina (Aguirre et al. 2018), Helwingia chinensis (Yu et al. 2012b), and blueberry (Choi 2011; Yu et al. 2012a). To our knowledge, this is the first report of B. dothidea on G. sinensis in China.The author(s) declare no conflict of interest.References:Aguirre, N. M., et al. 2018. Plant Dis. 102:1451. https://doi.org/10.1094/PDIS-08-17-1322-PDN Link, ISI, Google ScholarChoi, I. Y. 2011. Plant Dis. 95:207. Google ScholarYu, L., et al. 2012a. Plant Dis. 96:1697. https://doi.org/10.1094/PDIS-05-12-0500-PDN Link, ISI, Google ScholarYu, L., et al. 2012b. Plant Dis. 96:1821. https://doi.org/10.1094/PDIS-03-12-0275-PDN Link, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: This research was supported by Yunnan Provincial Department of Science and Technology (2018HB100, 2017FH001-005, 2017FH001-035, 2019FH001-008).DetailsFiguresLiterature CitedRelated Vol. 105, No. 3 March 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionMaize ear showing scattered moldy or “starburst” symptoms, typical of Fusarium verticillioides (A. F. Logrieco et al.). Photo credit: G. Munkvold. Bright yellow vein banding, rings, and lines associated with alfalfa mosaic virus infection in chayote (Sechium edule (Jacq.) Sw.) (G. Parrella et al.). Photo credit: G. Parrella. Metrics Article History Issue Date: 26 Mar 2021Published: 25 Jan 2021First Look: 6 Oct 2020Accepted: 2 Oct 2020 Pages: 706-706 Information© 2021 The American Phytopathological SocietyFundingYunnan Provincial Department of Science and TechnologyGrant/Award Number: 2018HB100Grant/Award Number: 2017FH001-005Grant/Award Number: 2017FH001-035Grant/Award Number: 2019FH001-008Keywordsfungitreesforestepidemiologydisease development and spreadThe author(s) declare no conflict of interest.PDF downloadCited byThe first detection of Botryosphaeria sinensis on white lupinePLANT PROTECTION NEWS, Vol. 104, No. 4" @default.
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