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• W3092017259 abstract "Abstract There is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, multiphoton microscope, driven by a fiber-based ultrafast laser, enabling excitation wavelengths at 775 nm and 1300 nm for nonlinear optical imaging, which simultaneously records second-harmonic generation, third-harmonic generation and three-photon excited ultraviolet fluorescence to identify and detect protein crystals with high sensitivity. The instrument serves as a valuable and important tool supporting sample scoring and sample optimization in biomolecular crystallography, which we hope will increase the capabilities and productivity of serial diffraction data collection in the future." @default.
• W3092017259 created "2020-10-15" @default.
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• W3092017259 date "2020-10-13" @default.
• W3092017259 modified "2023-10-14" @default.
• W3092017259 title "Protein-crystal detection with a compact multimodal multiphoton microscope" @default.
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• W3092017259 doi "https://doi.org/10.1038/s42003-020-01275-8" @default.
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