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- W3092162281 abstract "RNAs form critical components of biological processes implicated in human diseases, making them attractive for small-molecule therapeutics. Expanding the sites accessible to nuclear magnetic resonance (NMR) spectroscopy will provide atomic-level insights into RNA interactions. Here, we present an efficient strategy to introduce 19F-13C spin pairs into RNA by using a 5-fluorouridine-5'-triphosphate and T7 RNA polymerase-based in vitro transcription. Incorporating the 19F-13C label in two model RNAs produces linewidths that are twice as sharp as the commonly used 1H-13C spin pair. Furthermore, the high sensitivity of the 19F nucleus allows for clear delineation of helical and nonhelical regions as well as GU wobble and Watson-Crick base pairs. Last, the 19F-13C label enables rapid identification of a small-molecule binding pocket within human hepatitis B virus encapsidation signal epsilon (hHBV ε) RNA. We anticipate that the methods described herein will expand the size limitations of RNA NMR and aid with RNA-drug discovery efforts." @default.
- W3092162281 created "2020-10-15" @default.
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- W3092162281 date "2020-10-09" @default.
- W3092162281 modified "2023-10-15" @default.
- W3092162281 title "Solution NMR readily reveals distinct structural folds and interactions in doubly<sup>13</sup>C- and<sup>19</sup>F-labeled RNAs" @default.
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- W3092162281 doi "https://doi.org/10.1126/sciadv.abc6572" @default.
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- W3092162281 hasPublicationYear "2020" @default.
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