FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3092320908> ?p ?o ?g. }

• W3092320908 abstract "ABSTRACT The reactive oxygen species produced during inflammation through the neutrophilic respiratory burst play profound roles in combating bacterial pathogens and regulating the microbiota. Among these, the neutrophilic oxidant bleach, hypochlorous acid (HOCl), is the most prevalent and strongest oxidizer and kills bacteria through non-specific oxidation of proteins, lipids, and DNA. Thus, HOCl can be viewed as a host-specific cue that conveys important information about what bacterial physiology and lifestyle programs may be required for successful colonization. Nevertheless, bacteria that colonize animals face a molecular challenge in how to achieve highly selective detection of HOCl due to its reactive and transient nature and chemical similarity to more benign and non-host-specific oxidants like hydrogen peroxide (H 2 O 2 ). Here, we report that in response to increasing HOCl levels E. coli regulates biofilm production via activation of the diguanylate cyclase DgcZ. We show the molecular mechanism of this activation to be specific oxidation of a conserved cysteine that coordinates the zinc of its regulatory chemoreceptor zinc-binding (CZB) domain, forming a zinc-cysteine redox switch 685-fold more sensitive to oxidation by HOCl over H 2 O 2 . Dissection of the signal transduction mechanism through quantum mechanics, molecular dynamics, and biochemical analyses reveal how the cysteine redox state alters the delicate equilibrium of competition for Zn ++ between the CZB domain and other zinc binders to relay the presence of HOCl through activating the associated GGDEF domain to catalyze c-di-GMP. We find biofilm formation and HOCl-sensing in vivo to be regulated by the conserved cysteine, and point mutants that mimic oxidized CZB states increase production of the biofilm matrix polymer poly-N-acetylglucosamine and total biofilm. We observe CZB-regulated diguanylate cyclases and chemoreceptors in phyla in which host-associated bacteria are prevalent and are possessed by pathogens that manipulate host inflammation as part of their colonization strategy. A phylogenetic survey of all known CZB sequences shows these domains to be conserved and widespread across diverse phyla, suggesting CZB origin predates the bacterial last universal common ancestor. The ability of bacteria to use CZB protein domains to perceive and thwart the host neutrophilic respiratory burst has implications for understanding the mechanisms of diseases of chronic inflammation and gut dysbiosis." @default.
• W3092320908 created "2020-10-15" @default.
• W3092320908 creator A5002840484 @default.
• W3092320908 creator A5002943998 @default.
• W3092320908 creator A5005344445 @default.
• W3092320908 creator A5005783260 @default.
• W3092320908 creator A5022373562 @default.
• W3092320908 creator A5027770199 @default.
• W3092320908 creator A5048098447 @default.
• W3092320908 creator A5060714245 @default.
• W3092320908 creator A5074509343 @default.
• W3092320908 date "2020-10-11" @default.
• W3092320908 modified "2023-09-26" @default.
• W3092320908 title "A bacterial inflammation sensor regulates c-di-GMP signaling, adhesion, and biofilm formation" @default.
• W3092320908 cites W1519266993 @default.
• W3092320908 cites W1531285870 @default.
• W3092320908 cites W1549181628 @default.
• W3092320908 cites W1620160353 @default.
• W3092320908 cites W1909924417 @default.
• W3092320908 cites W1966792574 @default.
• W3092320908 cites W1968113723 @default.
• W3092320908 cites W1968226573 @default.
• W3092320908 cites W1975084858 @default.
• W3092320908 cites W1983972705 @default.
• W3092320908 cites W1987861061 @default.
• W3092320908 cites W1994163777 @default.
• W3092320908 cites W1995106753 @default.
• W3092320908 cites W2003268469 @default.
• W3092320908 cites W2003662926 @default.
• W3092320908 cites W2008708467 @default.
• W3092320908 cites W2008720828 @default.
• W3092320908 cites W2012191446 @default.
• W3092320908 cites W2022948175 @default.
• W3092320908 cites W2025694904 @default.
• W3092320908 cites W2029587433 @default.
• W3092320908 cites W2029667189 @default.
• W3092320908 cites W2034400607 @default.
• W3092320908 cites W2040921311 @default.
• W3092320908 cites W2042274557 @default.
• W3092320908 cites W2042662175 @default.
• W3092320908 cites W2043986265 @default.
• W3092320908 cites W2048686249 @default.
• W3092320908 cites W2049650560 @default.
• W3092320908 cites W2058046187 @default.
• W3092320908 cites W2058363175 @default.
• W3092320908 cites W2058531000 @default.
• W3092320908 cites W2061448692 @default.
• W3092320908 cites W2061588529 @default.
• W3092320908 cites W2067011309 @default.
• W3092320908 cites W2069282308 @default.
• W3092320908 cites W2082199163 @default.
• W3092320908 cites W2084120324 @default.
• W3092320908 cites W2086323657 @default.
• W3092320908 cites W2086651923 @default.
• W3092320908 cites W2086829247 @default.
• W3092320908 cites W2087001688 @default.
• W3092320908 cites W2089903346 @default.
• W3092320908 cites W2094516986 @default.
• W3092320908 cites W2094642658 @default.
• W3092320908 cites W2097875133 @default.
• W3092320908 cites W2100148691 @default.
• W3092320908 cites W2100211989 @default.
• W3092320908 cites W2105329815 @default.
• W3092320908 cites W2107530746 @default.
• W3092320908 cites W2108700884 @default.
• W3092320908 cites W2109154308 @default.
• W3092320908 cites W2114196916 @default.
• W3092320908 cites W2122836009 @default.
• W3092320908 cites W2133179696 @default.
• W3092320908 cites W2136138987 @default.
• W3092320908 cites W2138682249 @default.
• W3092320908 cites W2139576605 @default.
• W3092320908 cites W2143981217 @default.
• W3092320908 cites W2146341019 @default.
• W3092320908 cites W2150697053 @default.
• W3092320908 cites W2150981663 @default.
• W3092320908 cites W2154900915 @default.
• W3092320908 cites W2158266834 @default.
• W3092320908 cites W2167279371 @default.
• W3092320908 cites W2167591869 @default.
• W3092320908 cites W2169103574 @default.
• W3092320908 cites W2169330513 @default.
• W3092320908 cites W2316847228 @default.
• W3092320908 cites W2320161747 @default.
• W3092320908 cites W2331570590 @default.
• W3092320908 cites W2334821946 @default.
• W3092320908 cites W2344658806 @default.
• W3092320908 cites W2408107261 @default.
• W3092320908 cites W2464482301 @default.
• W3092320908 cites W2477940784 @default.
• W3092320908 cites W2510319321 @default.
• W3092320908 cites W2578472495 @default.
• W3092320908 cites W2766175482 @default.
• W3092320908 cites W2766600278 @default.
• W3092320908 cites W2767100753 @default.
• W3092320908 cites W2767267823 @default.
• W3092320908 cites W2792694279 @default.
• W3092320908 cites W2801031128 @default.
• W3092320908 cites W2890128405 @default.
• W3092320908 cites W2893080428 @default.

• next