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- W3092395383 abstract "The study demonstrates the use of chitosan as a carrier agent of designed antifungal formulation (CME 4:1:1) based on a combination of plant compounds such as trans- cinnamaldehyde (C), methyl eugenol (M), and estragole (E). The formulation was encapsulated inside the chitosan biopolymer nanomatrix (Ne-CME) and characterized by SEM, FTIR, and XRD. The Ne-CME exhibited enhanced antifungal and aflatoxin B 1 inhibitory effect compared to the individual compounds and unencapsulated form. Ne-CME (0.04 μl/ml) caused significant protection of Piper longum fruit from fungal (90.05%) and aflatoxin B 1 (100%) contamination and had no significant negative effects on its nutritional properties. In addition, the probable antifungal mechanism of Ne-CME was investigated using in-silico (effect on Omt-1 and Vbs structural genes of AFB 1 biosynthesis) and biochemical (perturbances in the cell membrane, carbohydrate catabolism, methyl-glyoxal, mitochondrial membrane potential, and antioxidant defense system) assay. • A novel antifungal formulation (CME) was designed using statistical model. • Formulation (CME) was encapsulated into chitosan nanoparticles. • Antifungal mechanism of action of CME was studied. • Antifungal effectiveness of encapsulated CME was validated in food system. • Recommended encapsulated CME as a green food preservatives." @default.
- W3092395383 created "2020-10-15" @default.
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- W3092395383 date "2020-12-01" @default.
- W3092395383 modified "2023-09-24" @default.
- W3092395383 title "Fabrication of volatile compounds loaded-chitosan biopolymer nanoparticles: Optimization, characterization and assessment against Aspergillus flavus and aflatoxin B1 contamination" @default.
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- W3092395383 doi "https://doi.org/10.1016/j.ijbiomac.2020.09.257" @default.
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