Matches in SemOpenAlex for { <https://semopenalex.org/work/W3092416032> ?p ?o ?g. }
- W3092416032 endingPage "18" @default.
- W3092416032 startingPage "1" @default.
- W3092416032 abstract "Hypoxic Pulmonary Vasoconstriction (HPV) is an important physiological mechanism of the lungs that matches perfusion to ventilation thus maximizing O 2 saturation of the venous blood within the lungs. This study emphasizes on principal pathways in the initiation and modulation of hypoxic pulmonary vasoconstriction with a primary focus on the role of Ca 2+ signaling and Ca 2+ influx pathways in hypoxic pulmonary vasoconstriction. We used an ex vivo model, isolated perfused/ventilated mouse lung to evaluate hypoxic pulmonary vasoconstriction. Alveolar hypoxia (utilizing a mini ventilator) rapidly and reversibly increased pulmonary arterial pressure due to hypoxic pulmonary vasoconstriction in the isolated perfused/ventilated lung. By applying specific inhibitors for different membrane receptors and ion channels through intrapulmonary perfusion solution in isolated lung, we were able to define the targeted receptors and channels that regulate hypoxic pulmonary vasoconstriction. We show that extracellular Ca 2+ or Ca 2+ influx through various Ca 2+ ‐permeable channels in the plasma membrane is required for hypoxic pulmonary vasoconstriction. Removal of extracellular Ca 2+ abolished hypoxic pulmonary vasoconstriction, while blockade of L‐type voltage‐dependent Ca 2+ channels (with nifedipine), non‐selective cation channels (with 30 µM SKF‐96365), and TRPC6/TRPV1 channels (with 1 µM SAR‐7334 and 30 µM capsazepine, respectively) significantly and reversibly inhibited hypoxic pulmonary vasoconstriction. Furthermore, blockers of Ca 2+ ‐sensing receptors (by 30 µM NPS2143, an allosteric Ca 2+ ‐sensing receptors inhibitor) and Notch (by 30 µM DAPT, a γ‐secretase inhibitor) also attenuated hypoxic pulmonary vasoconstriction. These data indicate that Ca 2+ influx in pulmonary arterial smooth muscle cells through voltage‐dependent, receptor‐operated, and store‐operated Ca 2+ entry pathways all contribute to initiation of hypoxic pulmonary vasoconstriction. The extracellular Ca 2+ ‐mediated activation of Ca 2+ ‐sensing receptors and the cell–cell interaction via Notch ligands and receptors contribute to the regulation of hypoxic pulmonary vasoconstriction." @default.
- W3092416032 created "2020-10-15" @default.
- W3092416032 creator A5000852601 @default.
- W3092416032 creator A5001162420 @default.
- W3092416032 creator A5001408192 @default.
- W3092416032 creator A5011066154 @default.
- W3092416032 creator A5030895628 @default.
- W3092416032 creator A5034938550 @default.
- W3092416032 creator A5039381731 @default.
- W3092416032 creator A5047479450 @default.
- W3092416032 creator A5056829553 @default.
- W3092416032 creator A5064026168 @default.
- W3092416032 creator A5064441182 @default.
- W3092416032 creator A5069603319 @default.
- W3092416032 creator A5070187864 @default.
- W3092416032 creator A5072202652 @default.
- W3092416032 creator A5076803549 @default.
- W3092416032 creator A5077651225 @default.
- W3092416032 date "2020-10-01" @default.
- W3092416032 modified "2023-10-14" @default.
- W3092416032 title "Revisiting the mechanism of hypoxic pulmonary vasoconstriction using isolated perfused/ventilated mouse lung" @default.
- W3092416032 cites W1484561603 @default.
- W3092416032 cites W1488409237 @default.
- W3092416032 cites W1504123405 @default.
- W3092416032 cites W1523706411 @default.
- W3092416032 cites W1526317775 @default.
- W3092416032 cites W1536342083 @default.
- W3092416032 cites W1549316628 @default.
- W3092416032 cites W1568400196 @default.
- W3092416032 cites W1795554797 @default.
- W3092416032 cites W1841414574 @default.
- W3092416032 cites W1876419424 @default.
- W3092416032 cites W1876864122 @default.
- W3092416032 cites W1894088205 @default.
- W3092416032 cites W1921440755 @default.
- W3092416032 cites W1926185647 @default.
- W3092416032 cites W1930588275 @default.
- W3092416032 cites W1951323910 @default.
- W3092416032 cites W1964403908 @default.
- W3092416032 cites W1965907479 @default.
- W3092416032 cites W1973901622 @default.
- W3092416032 cites W1974029350 @default.
- W3092416032 cites W1982067488 @default.
- W3092416032 cites W1986089886 @default.
- W3092416032 cites W1987251432 @default.
- W3092416032 cites W1988860455 @default.
- W3092416032 cites W1991090418 @default.
- W3092416032 cites W1995272882 @default.
- W3092416032 cites W1998332595 @default.
- W3092416032 cites W1999277369 @default.
- W3092416032 cites W1999887461 @default.
- W3092416032 cites W2000797437 @default.
- W3092416032 cites W2001409949 @default.
- W3092416032 cites W2002439304 @default.
- W3092416032 cites W2008552098 @default.
- W3092416032 cites W2011995481 @default.
- W3092416032 cites W2016280101 @default.
- W3092416032 cites W2020123105 @default.
- W3092416032 cites W2020706943 @default.
- W3092416032 cites W2021144893 @default.
- W3092416032 cites W2021821416 @default.
- W3092416032 cites W2022613490 @default.
- W3092416032 cites W2025973200 @default.
- W3092416032 cites W2041234387 @default.
- W3092416032 cites W2048347960 @default.
- W3092416032 cites W2051152628 @default.
- W3092416032 cites W2059070349 @default.
- W3092416032 cites W2059304762 @default.
- W3092416032 cites W2069546580 @default.
- W3092416032 cites W2070667821 @default.
- W3092416032 cites W2071969879 @default.
- W3092416032 cites W2077511985 @default.
- W3092416032 cites W2078571865 @default.
- W3092416032 cites W2079674540 @default.
- W3092416032 cites W2080326827 @default.
- W3092416032 cites W2082153473 @default.
- W3092416032 cites W2088445533 @default.
- W3092416032 cites W2092587032 @default.
- W3092416032 cites W2095896860 @default.
- W3092416032 cites W2096327838 @default.
- W3092416032 cites W2099228824 @default.
- W3092416032 cites W2100577158 @default.
- W3092416032 cites W2106856165 @default.
- W3092416032 cites W2111983287 @default.
- W3092416032 cites W2112260532 @default.
- W3092416032 cites W2112543188 @default.
- W3092416032 cites W2112861506 @default.
- W3092416032 cites W2112929115 @default.
- W3092416032 cites W2113656210 @default.
- W3092416032 cites W2114167944 @default.
- W3092416032 cites W2117597411 @default.
- W3092416032 cites W2117796487 @default.
- W3092416032 cites W2118331826 @default.
- W3092416032 cites W2121460090 @default.
- W3092416032 cites W2123839214 @default.
- W3092416032 cites W2125319682 @default.
- W3092416032 cites W2125514019 @default.
- W3092416032 cites W2128213692 @default.