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- W3092468680 abstract "Agrobacterium-mediated transformation of canola (Brassica napus) via hypocotyl segments has been a commonly used method for the past 30 years. While the hypocotyl-based method is well-established, it is not readily adapted to elite germplasm and the prolonged process is not ideal for a production transformation setting. We developed an Agrobacterium-mediated transformation method based on epicotyl and higher stem (internodal) segments that is efficient, rapid, and amenable for high-throughput transformation and genome editing. The method has been successfully implemented in multiple canola genotypes. The method appears to be genotype-independent, with varying transformation efficiencies. Internodal segment transformation was used to generate transgenic events as well as CRISPR-Cas9-mediated frameshift gene knockouts." @default.
- W3092468680 created "2020-10-15" @default.
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- W3092468680 date "2020-10-08" @default.
- W3092468680 modified "2023-10-16" @default.
- W3092468680 title "Genotype-Independent Transformation and Genome Editing of Brassica napus Using a Novel Explant Material" @default.
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- W3092468680 doi "https://doi.org/10.3389/fpls.2020.579524" @default.
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