FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3092562145> ?p ?o ?g. }

• W3092562145 endingPage "68" @default.
• W3092562145 startingPage "60" @default.
• W3092562145 abstract "A powerful and fast glycopeptide/glycan enrichment method is critical for the efficiency and throughput of mass spectrometry (MS)-based glycoproteomic and glycomic analyses, especially for large-scale sample analysis. Here, we report an ultrafast and effective method for both intact N-glycopeptide and N-glycan enrichment and apply it to human serum samples. In this method, a natural hydrophilic material, bacterial cellulose (BC), was adopted and fully optimized for enrichment. This method offers the following advantages: (i) The enrichment material has natural hydrophilicity and is low-cost, biocompatible, biodegradable and easily accessible; (ii) the whole enrichment procedure is remarkably simple and fast. It takes only 10 min for intact glycopeptides/glycans to be easily purified from mixtures; (iii) the specificity of this method is over 94% for both glycan and glycopeptide enrichment; and (iv) the outstanding specificity of this technique enables high isolation efficiency for the enrichment of both intact glycopeptides and glycans. A total of 36 N-glycans and 31 N-glycopeptides were identified from human immunoglobulin G (IgG). The glycan and glycopeptide absorption capacity of BC was as high as 333 μg/mg and 250 μg/mg (IgG/BC) respectively. The selectivity for glycan and glycopeptide enrichment reached 1:100 (IgG/bovine serum albumin (BSA), molar ratio) and 1:200 (maltoheptaose (DP7)/BSA, molar ratio), respectively. Furthermore, a total of 159 N-glycans and 523 N-glycopeptides were identified in human serum by using this method. Overall, the BC-based enrichment method we present here provides an ultrafast and highly efficient method for the enrichment of both N-glycopeptides and N-glycans in complex samples and shows great potential in large-scale glycoproteomic and glycomic analyses." @default.
• W3092562145 created "2020-10-15" @default.
• W3092562145 creator A5014402433 @default.
• W3092562145 creator A5014920470 @default.
• W3092562145 creator A5041754114 @default.
• W3092562145 creator A5051500470 @default.
• W3092562145 creator A5054493990 @default.
• W3092562145 creator A5057539657 @default.
• W3092562145 creator A5059446924 @default.
• W3092562145 creator A5059765034 @default.
• W3092562145 date "2020-12-01" @default.
• W3092562145 modified "2023-10-14" @default.
• W3092562145 title "An ultrafast and highly efficient enrichment method for both N-Glycopeptides and N-Glycans by bacterial cellulose" @default.
• W3092562145 cites W1798165859 @default.
• W3092562145 cites W1971159119 @default.
• W3092562145 cites W1978929408 @default.
• W3092562145 cites W1982936666 @default.
• W3092562145 cites W1985017852 @default.
• W3092562145 cites W2005767430 @default.
• W3092562145 cites W2016027337 @default.
• W3092562145 cites W2022754675 @default.
• W3092562145 cites W2024191848 @default.
• W3092562145 cites W2034632123 @default.
• W3092562145 cites W2042523679 @default.
• W3092562145 cites W2045510002 @default.
• W3092562145 cites W2046033544 @default.
• W3092562145 cites W2047004418 @default.
• W3092562145 cites W2068060920 @default.
• W3092562145 cites W2068922516 @default.
• W3092562145 cites W2068980146 @default.
• W3092562145 cites W2082162523 @default.
• W3092562145 cites W2100518445 @default.
• W3092562145 cites W2123960992 @default.
• W3092562145 cites W2217317199 @default.
• W3092562145 cites W2247651258 @default.
• W3092562145 cites W2311800161 @default.
• W3092562145 cites W2314318962 @default.
• W3092562145 cites W2315159203 @default.
• W3092562145 cites W2338266679 @default.
• W3092562145 cites W2590254004 @default.
• W3092562145 cites W2601885168 @default.
• W3092562145 cites W2615718202 @default.
• W3092562145 cites W2736592514 @default.
• W3092562145 cites W2807095067 @default.
• W3092562145 cites W2883650335 @default.
• W3092562145 cites W2888640582 @default.
• W3092562145 cites W2897052519 @default.
• W3092562145 cites W2901052186 @default.
• W3092562145 cites W2901145912 @default.
• W3092562145 cites W2944253192 @default.
• W3092562145 cites W2953689825 @default.
• W3092562145 cites W2966502138 @default.
• W3092562145 cites W2970852626 @default.
• W3092562145 cites W2999819052 @default.
• W3092562145 cites W3000438309 @default.
• W3092562145 cites W5908806 @default.
• W3092562145 doi "https://doi.org/10.1016/j.aca.2020.10.006" @default.
• W3092562145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33218490" @default.
• W3092562145 hasPublicationYear "2020" @default.
• W3092562145 type Work @default.
• W3092562145 sameAs 3092562145 @default.
• W3092562145 citedByCount "10" @default.
• W3092562145 countsByYear W30925621452021 @default.
• W3092562145 countsByYear W30925621452022 @default.
• W3092562145 countsByYear W30925621452023 @default.
• W3092562145 crossrefType "journal-article" @default.
• W3092562145 hasAuthorship W3092562145A5014402433 @default.
• W3092562145 hasAuthorship W3092562145A5014920470 @default.
• W3092562145 hasAuthorship W3092562145A5041754114 @default.
• W3092562145 hasAuthorship W3092562145A5051500470 @default.
• W3092562145 hasAuthorship W3092562145A5054493990 @default.
• W3092562145 hasAuthorship W3092562145A5057539657 @default.
• W3092562145 hasAuthorship W3092562145A5059446924 @default.
• W3092562145 hasAuthorship W3092562145A5059765034 @default.
• W3092562145 hasConcept C108625454 @default.
• W3092562145 hasConcept C137610392 @default.
• W3092562145 hasConcept C185592680 @default.
• W3092562145 hasConcept C206212055 @default.
• W3092562145 hasConcept C43617362 @default.
• W3092562145 hasConcept C501593827 @default.
• W3092562145 hasConcept C55493867 @default.
• W3092562145 hasConcept C86807702 @default.
• W3092562145 hasConceptScore W3092562145C108625454 @default.
• W3092562145 hasConceptScore W3092562145C137610392 @default.
• W3092562145 hasConceptScore W3092562145C185592680 @default.
• W3092562145 hasConceptScore W3092562145C206212055 @default.
• W3092562145 hasConceptScore W3092562145C43617362 @default.
• W3092562145 hasConceptScore W3092562145C501593827 @default.
• W3092562145 hasConceptScore W3092562145C55493867 @default.
• W3092562145 hasConceptScore W3092562145C86807702 @default.
• W3092562145 hasFunder F4320321001 @default.
• W3092562145 hasFunder F4320335777 @default.
• W3092562145 hasLocation W30925621451 @default.
• W3092562145 hasOpenAccess W3092562145 @default.
• W3092562145 hasPrimaryLocation W30925621451 @default.
• W3092562145 hasRelatedWork W1874751358 @default.
• W3092562145 hasRelatedWork W2070260909 @default.
• W3092562145 hasRelatedWork W2142420897 @default.

• next