Matches in SemOpenAlex for { <https://semopenalex.org/work/W3092666268> ?p ?o ?g. }
- W3092666268 endingPage "e05230" @default.
- W3092666268 startingPage "e05230" @default.
- W3092666268 abstract "Radioimmunoassay (RIA) methods have always represented a technique of choice for the determination of steroids in biological samples. The Amplified Luminescent Proximity Homogenous Assay-Linked Immunosorbent Assay (AlphaLISA) is now emerging as the new-generation immunoassay technology that does not require washing/separation steps. The aim of this study was to adapt the Perkin-Elmer's AlphaLISA kit for wool cortisol and compare it with a RIA wool cortisol assay. Wool from lambs, 35 at birth (A0) and 54 at two months old (A2), was collected and each extract was evaluated for wool cortisol concentrations (HCC) both by RIA and AlphaLISA immunoassay. The two methods showed good precision, sensitivity and specificity for determining HCC. Both methods were able to detect significant differences between the high and the low HCC assessed in lambs at A0 and A2 (P < 0.01). The HCC assessed with RIA were significantly higher than those assessed with AlphaLISA (P < 0.01). Moreover, the correlation between HCC measured using the AlphaLISA and RIA methods was strong (r = 0.878). The regression analyses show a constant and not proportional error. This could be due to the diversity in the dosage steps and to the diversity of the molecules used in the two methods. Results support the validity of using AlphaLISA as an alternative method to RIA for the quantification of cortisol in sheep wool and considering the performances showed it has a great potential to be further applied as an excellent tool to evaluate HCC in samples derived from animal species." @default.
- W3092666268 created "2020-10-22" @default.
- W3092666268 creator A5052588119 @default.
- W3092666268 creator A5056123301 @default.
- W3092666268 creator A5058947026 @default.
- W3092666268 creator A5060278387 @default.
- W3092666268 creator A5071378381 @default.
- W3092666268 date "2020-10-01" @default.
- W3092666268 modified "2023-09-28" @default.
- W3092666268 title "Comparison of AlphaLISA and RIA assays for measurement of wool cortisol concentrations" @default.
- W3092666268 cites W1542284571 @default.
- W3092666268 cites W1567661465 @default.
- W3092666268 cites W1587734848 @default.
- W3092666268 cites W1958999083 @default.
- W3092666268 cites W1968351242 @default.
- W3092666268 cites W1976757664 @default.
- W3092666268 cites W1994274623 @default.
- W3092666268 cites W2012310627 @default.
- W3092666268 cites W2051533226 @default.
- W3092666268 cites W2057929414 @default.
- W3092666268 cites W2072769184 @default.
- W3092666268 cites W2074337530 @default.
- W3092666268 cites W2074363521 @default.
- W3092666268 cites W2086338774 @default.
- W3092666268 cites W2141910921 @default.
- W3092666268 cites W2155945345 @default.
- W3092666268 cites W2160129580 @default.
- W3092666268 cites W2163386937 @default.
- W3092666268 cites W2178576449 @default.
- W3092666268 cites W2319880182 @default.
- W3092666268 cites W2354319758 @default.
- W3092666268 cites W2398472522 @default.
- W3092666268 cites W2416856247 @default.
- W3092666268 cites W2433586781 @default.
- W3092666268 cites W2513934301 @default.
- W3092666268 cites W2525585915 @default.
- W3092666268 cites W2617502138 @default.
- W3092666268 cites W2768771467 @default.
- W3092666268 cites W2793350103 @default.
- W3092666268 cites W2907506861 @default.
- W3092666268 cites W2912840571 @default.
- W3092666268 cites W2938306096 @default.
- W3092666268 cites W2950777255 @default.
- W3092666268 cites W2963321302 @default.
- W3092666268 cites W621251951 @default.
- W3092666268 doi "https://doi.org/10.1016/j.heliyon.2020.e05230" @default.
- W3092666268 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7569336" @default.
- W3092666268 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33102853" @default.
- W3092666268 hasPublicationYear "2020" @default.
- W3092666268 type Work @default.
- W3092666268 sameAs 3092666268 @default.
- W3092666268 citedByCount "7" @default.
- W3092666268 countsByYear W30926662682022 @default.
- W3092666268 countsByYear W30926662682023 @default.
- W3092666268 crossrefType "journal-article" @default.
- W3092666268 hasAuthorship W3092666268A5052588119 @default.
- W3092666268 hasAuthorship W3092666268A5056123301 @default.
- W3092666268 hasAuthorship W3092666268A5058947026 @default.
- W3092666268 hasAuthorship W3092666268A5060278387 @default.
- W3092666268 hasAuthorship W3092666268A5071378381 @default.
- W3092666268 hasBestOaLocation W30926662681 @default.
- W3092666268 hasConcept C159654299 @default.
- W3092666268 hasConcept C159985019 @default.
- W3092666268 hasConcept C176685330 @default.
- W3092666268 hasConcept C185592680 @default.
- W3092666268 hasConcept C192562407 @default.
- W3092666268 hasConcept C203014093 @default.
- W3092666268 hasConcept C2775951321 @default.
- W3092666268 hasConcept C2780420688 @default.
- W3092666268 hasConcept C43617362 @default.
- W3092666268 hasConcept C55493867 @default.
- W3092666268 hasConcept C86803240 @default.
- W3092666268 hasConceptScore W3092666268C159654299 @default.
- W3092666268 hasConceptScore W3092666268C159985019 @default.
- W3092666268 hasConceptScore W3092666268C176685330 @default.
- W3092666268 hasConceptScore W3092666268C185592680 @default.
- W3092666268 hasConceptScore W3092666268C192562407 @default.
- W3092666268 hasConceptScore W3092666268C203014093 @default.
- W3092666268 hasConceptScore W3092666268C2775951321 @default.
- W3092666268 hasConceptScore W3092666268C2780420688 @default.
- W3092666268 hasConceptScore W3092666268C43617362 @default.
- W3092666268 hasConceptScore W3092666268C55493867 @default.
- W3092666268 hasConceptScore W3092666268C86803240 @default.
- W3092666268 hasIssue "10" @default.
- W3092666268 hasLocation W30926662681 @default.
- W3092666268 hasLocation W30926662682 @default.
- W3092666268 hasLocation W30926662683 @default.
- W3092666268 hasLocation W30926662684 @default.
- W3092666268 hasLocation W30926662685 @default.
- W3092666268 hasOpenAccess W3092666268 @default.
- W3092666268 hasPrimaryLocation W30926662681 @default.
- W3092666268 hasRelatedWork W1955318616 @default.
- W3092666268 hasRelatedWork W1969688909 @default.
- W3092666268 hasRelatedWork W1994420353 @default.
- W3092666268 hasRelatedWork W1996200108 @default.
- W3092666268 hasRelatedWork W2008373365 @default.
- W3092666268 hasRelatedWork W2056391358 @default.
- W3092666268 hasRelatedWork W2139806284 @default.