Matches in SemOpenAlex for { <https://semopenalex.org/work/W3092736617> ?p ?o ?g. }
- W3092736617 abstract "Successful regeneration of genetically modified plants from cell culture is highly dependent on the species, genotype, and tissue-type being targeted for transformation. Studies in some plant species have shown that when expression is altered, some genes regulating developmental processes are capable of triggering plant regeneration in a variety of plant cells and tissue-types previously identified as being recalcitrant to regeneration. In the present research, we report that developmental genes encoding GROWTH-REGULATING FACTORS positively enhance regeneration and transformation in both monocot and dicot species. In sugar beet (Beta vulgaris ssp. vulgaris), ectopic expression of ArabidopsisGRF5 (AtGRF5) in callus cells accelerates shoot formation and dramatically increases transformation efficiency. More importantly, overexpression of AtGRF5 enables the production of stable transformants in recalcitrant sugar beet varieties. The introduction of AtGRF5 and GRF5 orthologs into canola (Brassica napus L.), soybean (Glycine max L.), and sunflower (Helianthus annuus L.) results in significant increases in genetic transformation of the explant tissue. A positive effect on proliferation of transgenic callus cells in canola was observed upon overexpression of GRF5 genes and AtGRF6 and AtGRF9. In soybean and sunflower, the overexpression of GRF5 genes seems to increase the proliferation of transformed cells, promoting transgenic shoot formation. In addition, the transformation of two putative AtGRF5 orthologs in maize (Zea mays L.) significantly boosts transformation efficiency and resulted in fully fertile transgenic plants. Overall, the results suggest that overexpression of GRF genes render cells and tissues more competent to regeneration across a wide variety of crop species and regeneration processes. This sets GRFs apart from other developmental regulators and, therefore, they can potentially be applied to improve transformation of monocot and dicot plant species." @default.
- W3092736617 created "2020-10-22" @default.
- W3092736617 creator A5014488860 @default.
- W3092736617 creator A5023321220 @default.
- W3092736617 creator A5034144036 @default.
- W3092736617 creator A5036380907 @default.
- W3092736617 creator A5038498908 @default.
- W3092736617 creator A5039652852 @default.
- W3092736617 creator A5042205390 @default.
- W3092736617 creator A5062008042 @default.
- W3092736617 creator A5080416864 @default.
- W3092736617 creator A5082780784 @default.
- W3092736617 creator A5088620958 @default.
- W3092736617 creator A5088962106 @default.
- W3092736617 date "2020-10-12" @default.
- W3092736617 modified "2023-10-12" @default.
- W3092736617 title "Overexpression of the Transcription Factor GROWTH-REGULATING FACTOR5 Improves Transformation of Dicot and Monocot Species" @default.
- W3092736617 cites W1533158369 @default.
- W3092736617 cites W1589150986 @default.
- W3092736617 cites W1596653005 @default.
- W3092736617 cites W1887277504 @default.
- W3092736617 cites W1953388725 @default.
- W3092736617 cites W1954252376 @default.
- W3092736617 cites W1965752081 @default.
- W3092736617 cites W1970583040 @default.
- W3092736617 cites W1971335363 @default.
- W3092736617 cites W1972411553 @default.
- W3092736617 cites W1975547288 @default.
- W3092736617 cites W1979264915 @default.
- W3092736617 cites W1990359266 @default.
- W3092736617 cites W2000846272 @default.
- W3092736617 cites W2006222065 @default.
- W3092736617 cites W2014775825 @default.
- W3092736617 cites W2039225213 @default.
- W3092736617 cites W2039783967 @default.
- W3092736617 cites W2039982136 @default.
- W3092736617 cites W2040319323 @default.
- W3092736617 cites W2052027914 @default.
- W3092736617 cites W2063851753 @default.
- W3092736617 cites W2065835623 @default.
- W3092736617 cites W2079268947 @default.
- W3092736617 cites W2079564769 @default.
- W3092736617 cites W2080314138 @default.
- W3092736617 cites W2082507214 @default.
- W3092736617 cites W2090978651 @default.
- W3092736617 cites W2102346306 @default.
- W3092736617 cites W2111025355 @default.
- W3092736617 cites W2114278286 @default.
- W3092736617 cites W2114437852 @default.
- W3092736617 cites W2115190641 @default.
- W3092736617 cites W2122087918 @default.
- W3092736617 cites W2123470208 @default.
- W3092736617 cites W2124596764 @default.
- W3092736617 cites W2129847122 @default.
- W3092736617 cites W2133130447 @default.
- W3092736617 cites W2140908794 @default.
- W3092736617 cites W2141344574 @default.
- W3092736617 cites W2141995813 @default.
- W3092736617 cites W2148750035 @default.
- W3092736617 cites W2154882486 @default.
- W3092736617 cites W2159675077 @default.
- W3092736617 cites W2167279371 @default.
- W3092736617 cites W2170185310 @default.
- W3092736617 cites W2171135463 @default.
- W3092736617 cites W2171198297 @default.
- W3092736617 cites W2346418876 @default.
- W3092736617 cites W2471889160 @default.
- W3092736617 cites W2474522654 @default.
- W3092736617 cites W2487652070 @default.
- W3092736617 cites W2495839614 @default.
- W3092736617 cites W2516035302 @default.
- W3092736617 cites W2518225655 @default.
- W3092736617 cites W2520452319 @default.
- W3092736617 cites W2732418005 @default.
- W3092736617 cites W2750458583 @default.
- W3092736617 cites W2767459576 @default.
- W3092736617 cites W2787383789 @default.
- W3092736617 cites W2801634803 @default.
- W3092736617 cites W2830007178 @default.
- W3092736617 cites W2868201791 @default.
- W3092736617 cites W2891528281 @default.
- W3092736617 cites W2910520253 @default.
- W3092736617 cites W2911231558 @default.
- W3092736617 cites W2911694359 @default.
- W3092736617 cites W2912154168 @default.
- W3092736617 cites W2913386392 @default.
- W3092736617 cites W2916900865 @default.
- W3092736617 cites W2942993307 @default.
- W3092736617 cites W2981056404 @default.
- W3092736617 cites W2988484581 @default.
- W3092736617 cites W3091894549 @default.
- W3092736617 cites W4211093584 @default.
- W3092736617 cites W4234219910 @default.
- W3092736617 cites W927937097 @default.
- W3092736617 doi "https://doi.org/10.3389/fpls.2020.572319" @default.
- W3092736617 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7585916" @default.
- W3092736617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33154762" @default.
- W3092736617 hasPublicationYear "2020" @default.
- W3092736617 type Work @default.
- W3092736617 sameAs 3092736617 @default.