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• W3092805346 abstract "Guarana is a tropical fruit that grows in the Amazon basin and the northeastern part of Brazil, from which a popular soft drink is manufactured. The main methylxanthine (MX) in the guarana powder is caffeine (CF); theobromine (TB) and theophylline (TP) are also present in much lower concentrations. Because of the popularity of this soft drink, and the stimulating effects of MXs, we introduced an undergraduate chemistry project on their extraction from guarana powder and subsequent analysis. A literature survey showed that there is some variation in the values of λmax and emax of the three MXs. Using purified authentic samples, we showed that values of λmax and emax of CF, TB and TP are very close. Consequently, their concentrations in guarana extract cannot be calculated using a single technique (UV-Vis). After discussing this problem with the students, the following project was carried out: (i) guarana powder was extracted with acidified aqueous ethanol under different experimental conditions (alcohol volume fraction in the binary solvent mixture, ; powder extraction time, t); (ii) the apparent CF concentration was calculated from the absorbance of the extract at λmax = 272.5 nm; (iii) the extracts were analyzed by high performance liquid chromatography; the three MXs were identified in the chromatograms using internal standards, and the true [CF] calculated. Stage (i) involved use of chemometrics to optimize powder extraction, and to get information on the relative importance of the experimental variables ( is more important than t). Stage (ii) was a straightforward application of Beer’s law. Stage (iii) demonstrated the power of using tandem techniques to solve complex, everyday situation, namely the separation and analysis of biologically active components in consumer products (soft- and energy drinks)." @default.
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• W3092805346 date "2020-09-15" @default.
• W3092805346 modified "2023-09-23" @default.
• W3092805346 title "Application of Chemometrics and Tandem Techniques in the Chemistry of Beverages: Analysis of Methylxanthines in Guaraná Powder Extract" @default.
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• W3092805346 doi "https://doi.org/10.5923/j.jlce.20200802.03" @default.
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