Matches in SemOpenAlex for { <https://semopenalex.org/work/W3092827092> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W3092827092 abstract "Background: An effective method to develop a safe vaccine against botulism is to utilize molecular biology techniques to produce recombinant antigens, which provoke the immune response in the recipient organism. A suggested antigen is a specific recombinant fragment of the botulinum neurotoxin (BoNT), which elicits the predictable immune response and does not have any toxic effects. In this study, the binding domain of the heavy chain of BoNT serotype A, which is the responsible subunit for binding to the receptor(s) of presynaptic membranes in neuromuscular junctions, is the selected fragment of this toxin to be recombinantly produced. Objectives: In order to prevent a severe syndrome such as Botulism, developing efficient vaccines against it is a necessity. Efforts have been made to accomplish this throughout time; however, some have discontinued due to the risks and unreliability of their production and usage. Methods: The encoding gene of BoNT/A-Hc was cloned into two different strains of Pichia pastoris, which were compared to each other based on the yield of the recombinant product. Results: The results demonstrated that the expression of recombinant BoNT/A-Hc by PichiaPink strain was successful, and the achieved recombinant BoNT/A-Hc was subsequently purified and then verified by using the specific antibody and analytical methods. Conclusions: In contrast, the expression results from the X-33 strain were not significant." @default.
- W3092827092 created "2020-10-22" @default.
- W3092827092 creator A5041553681 @default.
- W3092827092 creator A5044835281 @default.
- W3092827092 date "2020-10-13" @default.
- W3092827092 modified "2023-10-18" @default.
- W3092827092 title "Expression, Purification, and Verification of Recombinant Botulinum Neurotoxin Type A Binding Domain: A Comparison Between X33 and PichiaPink Strains of Pichia pastoris" @default.
- W3092827092 cites W1495524572 @default.
- W3092827092 cites W1775749144 @default.
- W3092827092 cites W1973515844 @default.
- W3092827092 cites W1988078730 @default.
- W3092827092 cites W1997003449 @default.
- W3092827092 cites W2004951521 @default.
- W3092827092 cites W2007982098 @default.
- W3092827092 cites W2018321735 @default.
- W3092827092 cites W2022104280 @default.
- W3092827092 cites W2033500343 @default.
- W3092827092 cites W2037136314 @default.
- W3092827092 cites W2041721711 @default.
- W3092827092 cites W2048207128 @default.
- W3092827092 cites W2056119251 @default.
- W3092827092 cites W2065075871 @default.
- W3092827092 cites W2068379293 @default.
- W3092827092 cites W2078541439 @default.
- W3092827092 cites W2088973286 @default.
- W3092827092 cites W2089344597 @default.
- W3092827092 cites W2108786034 @default.
- W3092827092 cites W2121439235 @default.
- W3092827092 cites W2122092296 @default.
- W3092827092 cites W2126444066 @default.
- W3092827092 cites W2138352514 @default.
- W3092827092 cites W2141028120 @default.
- W3092827092 cites W2158406618 @default.
- W3092827092 cites W2225433666 @default.
- W3092827092 cites W2238363303 @default.
- W3092827092 cites W2337450480 @default.
- W3092827092 cites W2740139987 @default.
- W3092827092 cites W2753299301 @default.
- W3092827092 cites W69916898 @default.
- W3092827092 doi "https://doi.org/10.5812/jjnpp.80447" @default.
- W3092827092 hasPublicationYear "2020" @default.
- W3092827092 type Work @default.
- W3092827092 sameAs 3092827092 @default.
- W3092827092 citedByCount "1" @default.
- W3092827092 countsByYear W30928270922023 @default.
- W3092827092 crossrefType "journal-article" @default.
- W3092827092 hasAuthorship W3092827092A5041553681 @default.
- W3092827092 hasAuthorship W3092827092A5044835281 @default.
- W3092827092 hasBestOaLocation W30928270921 @default.
- W3092827092 hasConcept C104317684 @default.
- W3092827092 hasConcept C108305142 @default.
- W3092827092 hasConcept C153911025 @default.
- W3092827092 hasConcept C159047783 @default.
- W3092827092 hasConcept C2777010668 @default.
- W3092827092 hasConcept C2777367657 @default.
- W3092827092 hasConcept C2779186577 @default.
- W3092827092 hasConcept C2779703480 @default.
- W3092827092 hasConcept C2780115692 @default.
- W3092827092 hasConcept C2780358027 @default.
- W3092827092 hasConcept C2781372317 @default.
- W3092827092 hasConcept C2994342964 @default.
- W3092827092 hasConcept C40767141 @default.
- W3092827092 hasConcept C55493867 @default.
- W3092827092 hasConcept C86803240 @default.
- W3092827092 hasConcept C89423630 @default.
- W3092827092 hasConceptScore W3092827092C104317684 @default.
- W3092827092 hasConceptScore W3092827092C108305142 @default.
- W3092827092 hasConceptScore W3092827092C153911025 @default.
- W3092827092 hasConceptScore W3092827092C159047783 @default.
- W3092827092 hasConceptScore W3092827092C2777010668 @default.
- W3092827092 hasConceptScore W3092827092C2777367657 @default.
- W3092827092 hasConceptScore W3092827092C2779186577 @default.
- W3092827092 hasConceptScore W3092827092C2779703480 @default.
- W3092827092 hasConceptScore W3092827092C2780115692 @default.
- W3092827092 hasConceptScore W3092827092C2780358027 @default.
- W3092827092 hasConceptScore W3092827092C2781372317 @default.
- W3092827092 hasConceptScore W3092827092C2994342964 @default.
- W3092827092 hasConceptScore W3092827092C40767141 @default.
- W3092827092 hasConceptScore W3092827092C55493867 @default.
- W3092827092 hasConceptScore W3092827092C86803240 @default.
- W3092827092 hasConceptScore W3092827092C89423630 @default.
- W3092827092 hasIssue "3" @default.
- W3092827092 hasLocation W30928270921 @default.
- W3092827092 hasOpenAccess W3092827092 @default.
- W3092827092 hasPrimaryLocation W30928270921 @default.
- W3092827092 hasRelatedWork W1583048023 @default.
- W3092827092 hasRelatedWork W2048444368 @default.
- W3092827092 hasRelatedWork W2160081225 @default.
- W3092827092 hasRelatedWork W22973672 @default.
- W3092827092 hasRelatedWork W2393693149 @default.
- W3092827092 hasRelatedWork W2605850300 @default.
- W3092827092 hasRelatedWork W2783911092 @default.
- W3092827092 hasRelatedWork W2914210430 @default.
- W3092827092 hasRelatedWork W2998458153 @default.
- W3092827092 hasRelatedWork W2113038636 @default.
- W3092827092 hasVolume "15" @default.
- W3092827092 isParatext "false" @default.
- W3092827092 isRetracted "false" @default.
- W3092827092 magId "3092827092" @default.
- W3092827092 workType "article" @default.