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- W3092837411 abstract "Abstract Background Endoplasmic reticulum (ER) stress is involved in the progression of Alzheimer’s disease (AD). Verbascoside (VB), an active phenylethanoid glycoside that was first isolated from Verbascum sinuatum (the wavyleaf mullein), possesses anti-inflammatory, antioxidative, and anti-apoptotic effects. The purpose of this study was to elucidate the beneficial effects of VB in amyloid β (Aβ) 1–42 -damaged human glioma (U251) cells and in APPswe/PSEN1dE9 transgenic (APP/PS1) mice. Methods U251 cells were co-incubated with 10 μM of Aβ 1-42 and treated with VB. The protective effects of VB were investigated by using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay, flow cytometry, fluorescence staining, and transmission electron microscopy. APP/PS1 transgenic mice were treated for 6 weeks with VB. Learning and memory were evaluated using a Morris water maze test. Immunohistochemistry, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling, thioflavin-S staining, and proteomics analysis were performed to study the potential neuroprotective mechanism. Enzyme-linked immunosorbent assays and western blot were performed to analyze altered protein levels of brain lysates in APP/PS1 mice and/or Aβ 1-42 -damaged U251 cells. Results In Aβ 1-42 -damaged U251 cells, VB significantly improved cell viability, inhibited apoptosis, reduced calcium accumulation and the intracellular concentrations of reactive oxygen species, and improved the morphology of mitochondria and ER. In APP/PS1 mice, 6-week administration of VB significantly improved memory and cognition. VB inhibited apoptosis, reduced the deposition of Aβ, reduced the formation of neurofibrillary tangles formed by hyperphosphorylated tau protein, and downregulated the expression levels of 4-hydroxynonenal and mesencephalic astrocyte-derived neurotrophic factor in the brains of APP/PS1 mice. Proteomics analysis of mouse hippocampus suggested that the neuroprotective effect of VB may be related to the reduction of ER stress. This was indicated by the fact that VB inhibited the three branches of the unfolded protein response, thereby attenuating ER stress and preventing apoptosis. Conclusions The results confirmed that VB possesses significant neuroprotective effects, which are related to the reduction of ER stress. These findings support the status of VB as a potentially effective treatment for AD and warrant further research." @default.
- W3092837411 created "2020-10-22" @default.
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- W3092837411 date "2020-10-18" @default.
- W3092837411 modified "2023-10-12" @default.
- W3092837411 title "Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice" @default.
- W3092837411 cites W1502618780 @default.
- W3092837411 cites W1563064469 @default.
- W3092837411 cites W1585299103 @default.
- W3092837411 cites W1604079405 @default.
- W3092837411 cites W1616122655 @default.
- W3092837411 cites W1919999795 @default.
- W3092837411 cites W1965514223 @default.
- W3092837411 cites W1975300361 @default.
- W3092837411 cites W1977434686 @default.
- W3092837411 cites W1990559695 @default.
- W3092837411 cites W1991936623 @default.
- W3092837411 cites W1993160420 @default.
- W3092837411 cites W1997019282 @default.
- W3092837411 cites W2009830344 @default.
- W3092837411 cites W2017406246 @default.
- W3092837411 cites W2021385892 @default.
- W3092837411 cites W2022210997 @default.
- W3092837411 cites W2024156871 @default.
- W3092837411 cites W2024264359 @default.
- W3092837411 cites W2026938853 @default.
- W3092837411 cites W2033645097 @default.
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- W3092837411 cites W2141043038 @default.
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- W3092837411 cites W2170069105 @default.
- W3092837411 cites W2252984568 @default.
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- W3092837411 cites W2439369296 @default.
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- W3092837411 doi "https://doi.org/10.1186/s12974-020-01976-1" @default.
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