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- W3092938863 abstract "The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized nickel ions, giving a highly selective interaction that allows purification of tagged proteins or protein complexes from <1% to >95% homogeneity in just one step (,). The tight association between the tag and the Ni-NTA resin allows contaminants to be easily washed away under stringent conditions, yet the bound proteins can be gently eluted by competition with imidazole, or a slight reduction in pH. Moreover, because the interaction is independent of the tertiary structure of the tag, 6xHis-tagged proteins can be purified even under the strongly denaturing conditions required to solubilize inclusion bodies." @default.
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- W3092938863 date "2003-11-14" @default.
- W3092938863 modified "2023-09-27" @default.
- W3092938863 title "One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin" @default.
- W3092938863 doi "https://doi.org/10.1385/0-89603-402-x:491" @default.
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