FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3093025246> ?p ?o ?g. }

• W3093025246 endingPage "1736" @default.
• W3093025246 startingPage "1727" @default.
• W3093025246 abstract "The world is currently facing a novel viral pandemic (SARS-CoV-2), and large-scale testing is central to decision-making for the design of effective policies and control strategies to minimize its impact on the global population. However, testing for the presence of the virus is a major bottleneck in tracking the spreading of the disease. Given its adaptability regarding the nucleotide sequence of target regions, RT-qPCR is a strong ally to reveal the rapid geographical spreading of novel viruses. We assessed PCR variations in the SARS-CoV-2 diagnosis taking into account public genome sequences and diagnosis kits used by different countries. We analyzed 226 SARS-CoV-2 genome sequences from samples collected by March 22, 2020. Our work utilizes a phylogenetic approach that reveals the early evolution of the virus sequence as it spreads around the globe and informs the design of RT-qPCR primers and probes. The quick expansion of testing capabilities of a country during a pandemic is largely impaired by the availability of adequately trained personnel on RNA isolation and PCR analysis, as well as the availability of hardware (thermocyclers). We propose that rapid capacity development can circumvent these bottlenecks by training medical and non-medical personnel with some laboratory experience, such as biology-related graduate students. Furthermore, the use of thermocyclers available in academic and commercial labs can be promptly calibrated and certified to properly conduct testing during a pandemic. A decentralized, fast-acting training and testing certification pipeline will better prepare us to manage future pandemics." @default.
• W3093025246 created "2020-10-22" @default.
• W3093025246 creator A5014806144 @default.
• W3093025246 creator A5050717760 @default.
• W3093025246 creator A5065655310 @default.
• W3093025246 creator A5085041277 @default.
• W3093025246 creator A5090165896 @default.
• W3093025246 date "2020-10-17" @default.
• W3093025246 modified "2023-10-17" @default.
• W3093025246 title "Diagnosing the novel SARS-CoV-2 by quantitative RT-PCR: variations and opportunities" @default.
• W3093025246 cites W1934338847 @default.
• W3093025246 cites W2000576539 @default.
• W3093025246 cites W2008597300 @default.
• W3093025246 cites W2009596137 @default.
• W3093025246 cites W2066048377 @default.
• W3093025246 cites W2072334214 @default.
• W3093025246 cites W2095864050 @default.
• W3093025246 cites W2101258647 @default.
• W3093025246 cites W2106882534 @default.
• W3093025246 cites W2110992157 @default.
• W3093025246 cites W2128110156 @default.
• W3093025246 cites W2311203695 @default.
• W3093025246 cites W2342696065 @default.
• W3093025246 cites W2509643246 @default.
• W3093025246 cites W2562961696 @default.
• W3093025246 cites W2600476130 @default.
• W3093025246 cites W2727690093 @default.
• W3093025246 cites W2789402589 @default.
• W3093025246 cites W2789538521 @default.
• W3093025246 cites W2796800715 @default.
• W3093025246 cites W2894653626 @default.
• W3093025246 cites W2944626270 @default.
• W3093025246 cites W2946387619 @default.
• W3093025246 cites W3001195213 @default.
• W3093025246 cites W3001975323 @default.
• W3093025246 cites W3003637715 @default.
• W3093025246 cites W3004239190 @default.
• W3093025246 cites W3006750069 @default.
• W3093025246 cites W3007678386 @default.
• W3093025246 cites W3007831031 @default.
• W3093025246 cites W3008443627 @default.
• W3093025246 cites W3008891488 @default.
• W3093025246 cites W3009219040 @default.
• W3093025246 cites W3011420630 @default.
• W3093025246 cites W3011662277 @default.
• W3093025246 cites W3012284084 @default.
• W3093025246 cites W3013776441 @default.
• W3093025246 cites W3013981415 @default.
• W3093025246 cites W3014222358 @default.
• W3093025246 cites W3015040045 @default.
• W3093025246 cites W3015444032 @default.
• W3093025246 cites W3016924174 @default.
• W3093025246 cites W3017669300 @default.
• W3093025246 cites W3020627814 @default.
• W3093025246 cites W3021396888 @default.
• W3093025246 cites W3021922824 @default.
• W3093025246 cites W3021971574 @default.
• W3093025246 cites W3022498108 @default.
• W3093025246 cites W3027129147 @default.
• W3093025246 cites W3027147731 @default.
• W3093025246 cites W3027866910 @default.
• W3093025246 cites W3028877158 @default.
• W3093025246 cites W3034963396 @default.
• W3093025246 cites W3038071236 @default.
• W3093025246 cites W3038903970 @default.
• W3093025246 cites W3041771467 @default.
• W3093025246 cites W3042504804 @default.
• W3093025246 cites W3045178645 @default.
• W3093025246 cites W3046218112 @default.
• W3093025246 cites W3049588438 @default.
• W3093025246 cites W3085721524 @default.
• W3093025246 cites W3094371751 @default.
• W3093025246 doi "https://doi.org/10.1007/s00109-020-01992-x" @default.
• W3093025246 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7567654" @default.
• W3093025246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33067676" @default.
• W3093025246 hasPublicationYear "2020" @default.
• W3093025246 type Work @default.
• W3093025246 sameAs 3093025246 @default.
• W3093025246 citedByCount "32" @default.
• W3093025246 countsByYear W30930252462021 @default.
• W3093025246 countsByYear W30930252462022 @default.
• W3093025246 countsByYear W30930252462023 @default.
• W3093025246 crossrefType "journal-article" @default.
• W3093025246 hasAuthorship W3093025246A5014806144 @default.
• W3093025246 hasAuthorship W3093025246A5050717760 @default.
• W3093025246 hasAuthorship W3093025246A5065655310 @default.
• W3093025246 hasAuthorship W3093025246A5085041277 @default.
• W3093025246 hasAuthorship W3093025246A5090165896 @default.
• W3093025246 hasBestOaLocation W30930252461 @default.
• W3093025246 hasConcept C104317684 @default.
• W3093025246 hasConcept C141231307 @default.
• W3093025246 hasConcept C142724271 @default.
• W3093025246 hasConcept C149635348 @default.
• W3093025246 hasConcept C159047783 @default.
• W3093025246 hasConcept C17744445 @default.
• W3093025246 hasConcept C199539241 @default.
• W3093025246 hasConcept C2775941552 @default.
• W3093025246 hasConcept C2779134260 @default.

• next