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- W3093042014 abstract "Mature mammalian oocytes contain lipid droplets (LDs), which are neutral lipid storage organelles critically important for energy metabolism. In mice, maternal obesity, induced by long-term (> 3 months) high-fat feeding, contributes to the accumulation of LDs in mature oocytes. However, few studies have investigated the influence of short-term high-fat feeding on LD content. In this study, we demonstrated that 3 weeks of high-fat feeding is sufficient to increase LD content and intracellular triacylglycerol levels. Using a two-step centrifugation technique to release LDs into the perivitelline space, we found that short-term high-fat feeding increased the level of LDs in MII oocytes and that 3 days of high-fat feeding were sufficient to increase efficiency of LD release. Collectively, our study suggests that short-term high fat feeding can have a higher impact on lipid metabolism during oocyte maturation." @default.
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- W3093042014 date "2021-01-01" @default.
- W3093042014 modified "2023-09-27" @default.
- W3093042014 title "Impact of short-term high-fat feeding on lipid droplet content in mouse oocytes" @default.
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- W3093042014 doi "https://doi.org/10.1262/jrd.2020-094" @default.
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