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• W3093077899 abstract "Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1–4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L." @default.
• W3093077899 created "2020-10-22" @default.
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• W3093077899 date "2020-10-14" @default.
• W3093077899 modified "2023-10-03" @default.
• W3093077899 title "Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani" @default.
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• W3093077899 doi "https://doi.org/10.3390/ijms21207601" @default.
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