FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3093175280> ?p ?o ?g. }

• W3093175280 endingPage "2315" @default.
• W3093175280 startingPage "2315" @default.
• W3093175280 abstract "Macroautophagy is a conserved degradative process for maintaining cellular homeostasis and plays a key role in aging and various human disorders. The microtubule-associated protein 1A/1B light chain 3B (MAP1LC3B or LC3B) is commonly analyzed as a key marker for autophagosomes and as a proxy for autophagic flux. Three paralogues of the LC3 gene exist in humans: LC3A, LC3B and LC3C. The molecular function, regulation and cellular localization of LC3A and LC3C have not been investigated frequently, even if a similar function to that described for LC3B appears likely. Here, we have selectively decapacitated LC3B by three separate strategies in primary human fibroblasts and analyzed the evoked effects on LC3A, LC3B and LC3C in terms of their cellular distribution and co-localization with p62, a ubiquitin and autophagy receptor. First, treatment with pharmacological sirtuin 1 (SIRT1) inhibitors to prevent the translocation of LC3B from the nucleus into the cytosol induced an increase in cytosolic LC3C, a heightened co-localization of LC3C with p62, and an increase LC3C-dependent autophagic flux as assessed by protein lipidation. Cytosolic LC3A, however, was moderately reduced, but also more co-localized with p62. Second, siRNA-based knock-down of SIRT1 broadly reproduced these findings and increased the co-localization of LC3A and particularly LC3C with p62 in presumed autophagosomes. These effects resembled the effects of pharmacological sirtuin inhibition under normal and starvation conditions. Third, siRNA-based knock-down of total LC3B in cytosol and nucleus also induced a redistribution of LC3C as if to replace LC3B in the nucleus, but only moderately affected LC3A. Total protein expression of LC3A, LC3B, LC3C, GABARAP and GABARAP-L1 following LC3B decapacitation was unaltered. Our data indicate that nuclear trapping and other causes of LC3B functional loss in the cytosol are buffered by LC3A and actively compensated by LC3C, but not by GABARAPs. The biological relevance of the potential functional compensation of LC3B decapacitation by LC3C and LC3A warrants further study." @default.
• W3093175280 created "2020-10-22" @default.
• W3093175280 creator A5002230610 @default.
• W3093175280 creator A5019356954 @default.
• W3093175280 creator A5024971580 @default.
• W3093175280 creator A5042970504 @default.
• W3093175280 creator A5049920694 @default.
• W3093175280 creator A5067236379 @default.
• W3093175280 creator A5070300628 @default.
• W3093175280 creator A5080397584 @default.
• W3093175280 date "2020-10-18" @default.
• W3093175280 modified "2023-10-16" @default.
• W3093175280 title "Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C" @default.
• W3093175280 cites W1555054913 @default.
• W3093175280 cites W1590083128 @default.
• W3093175280 cites W1985124617 @default.
• W3093175280 cites W1987769185 @default.
• W3093175280 cites W1990931780 @default.
• W3093175280 cites W1998019811 @default.
• W3093175280 cites W2002333772 @default.
• W3093175280 cites W2019666203 @default.
• W3093175280 cites W2025933441 @default.
• W3093175280 cites W2037127469 @default.
• W3093175280 cites W2040254484 @default.
• W3093175280 cites W2045970460 @default.
• W3093175280 cites W2095117493 @default.
• W3093175280 cites W2107537628 @default.
• W3093175280 cites W2111892050 @default.
• W3093175280 cites W2128162080 @default.
• W3093175280 cites W2156356581 @default.
• W3093175280 cites W2159591471 @default.
• W3093175280 cites W2300762239 @default.
• W3093175280 cites W2336330109 @default.
• W3093175280 cites W2508534437 @default.
• W3093175280 cites W2533025094 @default.
• W3093175280 cites W2555734739 @default.
• W3093175280 cites W2561316863 @default.
• W3093175280 cites W2565951690 @default.
• W3093175280 cites W2604819250 @default.
• W3093175280 cites W2752326209 @default.
• W3093175280 cites W2766025148 @default.
• W3093175280 cites W2769290777 @default.
• W3093175280 cites W2792870066 @default.
• W3093175280 cites W2799640787 @default.
• W3093175280 cites W2888605402 @default.
• W3093175280 cites W2909598539 @default.
• W3093175280 cites W4250359879 @default.
• W3093175280 doi "https://doi.org/10.3390/cells9102315" @default.
• W3093175280 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7603224" @default.
• W3093175280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33081014" @default.
• W3093175280 hasPublicationYear "2020" @default.
• W3093175280 type Work @default.
• W3093175280 sameAs 3093175280 @default.
• W3093175280 citedByCount "26" @default.
• W3093175280 countsByYear W30931752802021 @default.
• W3093175280 countsByYear W30931752802022 @default.
• W3093175280 countsByYear W30931752802023 @default.
• W3093175280 crossrefType "journal-article" @default.
• W3093175280 hasAuthorship W3093175280A5002230610 @default.
• W3093175280 hasAuthorship W3093175280A5019356954 @default.
• W3093175280 hasAuthorship W3093175280A5024971580 @default.
• W3093175280 hasAuthorship W3093175280A5042970504 @default.
• W3093175280 hasAuthorship W3093175280A5049920694 @default.
• W3093175280 hasAuthorship W3093175280A5067236379 @default.
• W3093175280 hasAuthorship W3093175280A5070300628 @default.
• W3093175280 hasAuthorship W3093175280A5080397584 @default.
• W3093175280 hasBestOaLocation W30931752801 @default.
• W3093175280 hasConcept C104317684 @default.
• W3093175280 hasConcept C119157956 @default.
• W3093175280 hasConcept C181199279 @default.
• W3093175280 hasConcept C185592680 @default.
• W3093175280 hasConcept C190062978 @default.
• W3093175280 hasConcept C190283241 @default.
• W3093175280 hasConcept C203522944 @default.
• W3093175280 hasConcept C25602115 @default.
• W3093175280 hasConcept C2776879804 @default.
• W3093175280 hasConcept C2779324830 @default.
• W3093175280 hasConcept C2780561631 @default.
• W3093175280 hasConcept C28859421 @default.
• W3093175280 hasConcept C49805395 @default.
• W3093175280 hasConcept C55493867 @default.
• W3093175280 hasConcept C86803240 @default.
• W3093175280 hasConcept C95444343 @default.
• W3093175280 hasConcept C98539663 @default.
• W3093175280 hasConceptScore W3093175280C104317684 @default.
• W3093175280 hasConceptScore W3093175280C119157956 @default.
• W3093175280 hasConceptScore W3093175280C181199279 @default.
• W3093175280 hasConceptScore W3093175280C185592680 @default.
• W3093175280 hasConceptScore W3093175280C190062978 @default.
• W3093175280 hasConceptScore W3093175280C190283241 @default.
• W3093175280 hasConceptScore W3093175280C203522944 @default.
• W3093175280 hasConceptScore W3093175280C25602115 @default.
• W3093175280 hasConceptScore W3093175280C2776879804 @default.
• W3093175280 hasConceptScore W3093175280C2779324830 @default.
• W3093175280 hasConceptScore W3093175280C2780561631 @default.
• W3093175280 hasConceptScore W3093175280C28859421 @default.
• W3093175280 hasConceptScore W3093175280C49805395 @default.
• W3093175280 hasConceptScore W3093175280C55493867 @default.

• next