Matches in SemOpenAlex for { <https://semopenalex.org/work/W3093300511> ?p ?o ?g. }
- W3093300511 endingPage "20.2020" @default.
- W3093300511 startingPage "ENEURO.0278" @default.
- W3093300511 abstract "Abstract Sec1/Munc18 proteins play a key role in initiating the assembly of N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, the molecular fusion machinery. Employing comparative structure modeling, site specific crosslinking by single amino acid substitutions with the photoactivatable unnatural amino acid p-Benzoyl-phenylalanine (Bpa) and reconstituted vesicle docking/fusion assays, we mapped the binding interface between Munc18-1 and the neuronal v-SNARE VAMP2 with single amino acid resolution. Our results show that helices 11 and 12 of domain 3a in Munc18-1 interact with the VAMP2 SNARE motif covering the region from layers −4 to +5. Residue Q301 in helix 11 plays a pivotal role in VAMP2 binding and template complex formation. A VAMP2 binding deficient mutant, Munc18-1 Q301D, does not stimulate lipid mixing in a reconstituted fusion assay. The neuronal SNARE-organizer Munc13-1, which also binds VAMP2, does not bypass the requirement for the Munc18-1·VAMP2 interaction. Importantly, Munc18-1 Q301D expression in Munc18-1 deficient neurons severely reduces synaptic transmission, demonstrating the physiological significance of the Munc18-1·VAMP2 interaction." @default.
- W3093300511 created "2020-10-22" @default.
- W3093300511 creator A5015928597 @default.
- W3093300511 creator A5018311085 @default.
- W3093300511 creator A5020682685 @default.
- W3093300511 creator A5026872836 @default.
- W3093300511 creator A5037327281 @default.
- W3093300511 creator A5039150236 @default.
- W3093300511 creator A5043321849 @default.
- W3093300511 creator A5056063291 @default.
- W3093300511 creator A5066246563 @default.
- W3093300511 creator A5076932666 @default.
- W3093300511 creator A5082246162 @default.
- W3093300511 date "2020-10-14" @default.
- W3093300511 modified "2023-10-18" @default.
- W3093300511 title "The Interaction of Munc18-1 Helix 11 and 12 with the Central Region of the VAMP2 SNARE Motif Is Essential for SNARE Templating and Synaptic Transmission" @default.
- W3093300511 cites W1497380575 @default.
- W3093300511 cites W1822005186 @default.
- W3093300511 cites W1822252127 @default.
- W3093300511 cites W1916164999 @default.
- W3093300511 cites W1970573820 @default.
- W3093300511 cites W1971607460 @default.
- W3093300511 cites W1973361899 @default.
- W3093300511 cites W1976479871 @default.
- W3093300511 cites W1978940699 @default.
- W3093300511 cites W1986826865 @default.
- W3093300511 cites W1987428952 @default.
- W3093300511 cites W2009761723 @default.
- W3093300511 cites W2013703566 @default.
- W3093300511 cites W2016516993 @default.
- W3093300511 cites W2024136850 @default.
- W3093300511 cites W2027775552 @default.
- W3093300511 cites W2031212561 @default.
- W3093300511 cites W2038133348 @default.
- W3093300511 cites W2040025072 @default.
- W3093300511 cites W2042089363 @default.
- W3093300511 cites W2047808133 @default.
- W3093300511 cites W2063487710 @default.
- W3093300511 cites W2067354100 @default.
- W3093300511 cites W2074369451 @default.
- W3093300511 cites W2076913495 @default.
- W3093300511 cites W2084367350 @default.
- W3093300511 cites W2086919645 @default.
- W3093300511 cites W2101097180 @default.
- W3093300511 cites W2103618752 @default.
- W3093300511 cites W2116535065 @default.
- W3093300511 cites W2120499783 @default.
- W3093300511 cites W2146322499 @default.
- W3093300511 cites W2153569460 @default.
- W3093300511 cites W2158456505 @default.
- W3093300511 cites W2159529647 @default.
- W3093300511 cites W2161367731 @default.
- W3093300511 cites W2272549607 @default.
- W3093300511 cites W2464112691 @default.
- W3093300511 cites W2581328787 @default.
- W3093300511 cites W2603594400 @default.
- W3093300511 cites W2611650009 @default.
- W3093300511 cites W2612109171 @default.
- W3093300511 cites W2657651830 @default.
- W3093300511 cites W2741524102 @default.
- W3093300511 cites W2769789189 @default.
- W3093300511 cites W2910595951 @default.
- W3093300511 cites W2940306262 @default.
- W3093300511 cites W2941374900 @default.
- W3093300511 cites W2951496883 @default.
- W3093300511 cites W2995314435 @default.
- W3093300511 cites W2996914143 @default.
- W3093300511 cites W3008624462 @default.
- W3093300511 cites W3011124312 @default.
- W3093300511 cites W3044504230 @default.
- W3093300511 cites W4211232835 @default.
- W3093300511 doi "https://doi.org/10.1523/eneuro.0278-20.2020" @default.
- W3093300511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7768276" @default.
- W3093300511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33055194" @default.
- W3093300511 hasPublicationYear "2020" @default.
- W3093300511 type Work @default.
- W3093300511 sameAs 3093300511 @default.
- W3093300511 citedByCount "18" @default.
- W3093300511 countsByYear W30933005112021 @default.
- W3093300511 countsByYear W30933005112022 @default.
- W3093300511 countsByYear W30933005112023 @default.
- W3093300511 crossrefType "journal-article" @default.
- W3093300511 hasAuthorship W3093300511A5015928597 @default.
- W3093300511 hasAuthorship W3093300511A5018311085 @default.
- W3093300511 hasAuthorship W3093300511A5020682685 @default.
- W3093300511 hasAuthorship W3093300511A5026872836 @default.
- W3093300511 hasAuthorship W3093300511A5037327281 @default.
- W3093300511 hasAuthorship W3093300511A5039150236 @default.
- W3093300511 hasAuthorship W3093300511A5043321849 @default.
- W3093300511 hasAuthorship W3093300511A5056063291 @default.
- W3093300511 hasAuthorship W3093300511A5066246563 @default.
- W3093300511 hasAuthorship W3093300511A5076932666 @default.
- W3093300511 hasAuthorship W3093300511A5082246162 @default.
- W3093300511 hasBestOaLocation W30933005111 @default.
- W3093300511 hasConcept C103038307 @default.
- W3093300511 hasConcept C2908790754 @default.
- W3093300511 hasConcept C41625074 @default.
- W3093300511 hasConcept C515207424 @default.