FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3093446251> ?p ?o ?g. }

• W3093446251 abstract "Abstract Cryo electron microscopy (cryo-EM), a key method for structure determination involves imaging purified material embedded in vitreous ice. Images are then computationally processed to obtain three-dimensional structures approaching atomic resolution. There is increasing interest in extending structural studies by cryo-EM into the cell, where biological structures and processes may be imaged in context. The limited penetrating power of electrons prevents imaging of thick specimens (> 500 nm) however. Cryo-sectioning methods employed to overcome this are technically challenging, subject to artefacts or involve specialised and costly equipment. Here we describe the first structure of herpesvirus capsids determined by sub-tomogram averaging from nuclei of eukaryotic cells, achieved by cryo-electron tomography (cryo-ET) of re-vitrified cell sections prepared using the Tokuyasu method. Our reconstructions confirm that the capsid associated tegument complex is present on capsids prior to nuclear egress. We demonstrate that this method is suited to both 3D structure determination and correlative light/electron microscopy, thus expanding the scope of cryogenic cellular imaging." @default.
• W3093446251 created "2020-10-22" @default.
• W3093446251 creator A5000794497 @default.
• W3093446251 creator A5001010613 @default.
• W3093446251 creator A5038820258 @default.
• W3093446251 creator A5062731020 @default.
• W3093446251 creator A5070140672 @default.
• W3093446251 date "2020-10-19" @default.
• W3093446251 modified "2023-10-01" @default.
• W3093446251 title "In situ structure of virus capsids within cell nuclei by correlative light and cryo-electron tomography" @default.
• W3093446251 cites W1541539119 @default.
• W3093446251 cites W1901313370 @default.
• W3093446251 cites W1945772050 @default.
• W3093446251 cites W1954470243 @default.
• W3093446251 cites W1964610374 @default.
• W3093446251 cites W1971080731 @default.
• W3093446251 cites W1972478587 @default.
• W3093446251 cites W1983346892 @default.
• W3093446251 cites W1995785876 @default.
• W3093446251 cites W1998535024 @default.
• W3093446251 cites W2003596982 @default.
• W3093446251 cites W2009863686 @default.
• W3093446251 cites W2016314394 @default.
• W3093446251 cites W2028651832 @default.
• W3093446251 cites W2029101220 @default.
• W3093446251 cites W2045671533 @default.
• W3093446251 cites W2066991125 @default.
• W3093446251 cites W2079358116 @default.
• W3093446251 cites W2081460107 @default.
• W3093446251 cites W2085010310 @default.
• W3093446251 cites W2086466935 @default.
• W3093446251 cites W2093542233 @default.
• W3093446251 cites W2096891475 @default.
• W3093446251 cites W2097484483 @default.
• W3093446251 cites W2100455255 @default.
• W3093446251 cites W2104234755 @default.
• W3093446251 cites W2126961090 @default.
• W3093446251 cites W2132629607 @default.
• W3093446251 cites W2141698142 @default.
• W3093446251 cites W2142161929 @default.
• W3093446251 cites W2142404794 @default.
• W3093446251 cites W2148872579 @default.
• W3093446251 cites W2150052212 @default.
• W3093446251 cites W2153750826 @default.
• W3093446251 cites W2153830973 @default.
• W3093446251 cites W2154780367 @default.
• W3093446251 cites W2165155404 @default.
• W3093446251 cites W2165922128 @default.
• W3093446251 cites W22921420 @default.
• W3093446251 cites W2303909136 @default.
• W3093446251 cites W2558525431 @default.
• W3093446251 cites W2607472780 @default.
• W3093446251 cites W2734356283 @default.
• W3093446251 cites W2767236644 @default.
• W3093446251 cites W2794757315 @default.
• W3093446251 cites W2796156307 @default.
• W3093446251 cites W2991096401 @default.
• W3093446251 doi "https://doi.org/10.1038/s41598-020-74104-x" @default.
• W3093446251 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7572381" @default.
• W3093446251 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33077791" @default.
• W3093446251 hasPublicationYear "2020" @default.
• W3093446251 type Work @default.
• W3093446251 sameAs 3093446251 @default.
• W3093446251 citedByCount "15" @default.
• W3093446251 countsByYear W30934462512021 @default.
• W3093446251 countsByYear W30934462512022 @default.
• W3093446251 countsByYear W30934462512023 @default.
• W3093446251 crossrefType "journal-article" @default.
• W3093446251 hasAuthorship W3093446251A5000794497 @default.
• W3093446251 hasAuthorship W3093446251A5001010613 @default.
• W3093446251 hasAuthorship W3093446251A5038820258 @default.
• W3093446251 hasAuthorship W3093446251A5062731020 @default.
• W3093446251 hasAuthorship W3093446251A5070140672 @default.
• W3093446251 hasBestOaLocation W30934462511 @default.
• W3093446251 hasConcept C120665830 @default.
• W3093446251 hasConcept C121332964 @default.
• W3093446251 hasConcept C12554922 @default.
• W3093446251 hasConcept C138268822 @default.
• W3093446251 hasConcept C138885662 @default.
• W3093446251 hasConcept C147080431 @default.
• W3093446251 hasConcept C151730666 @default.
• W3093446251 hasConcept C154945302 @default.
• W3093446251 hasConcept C159047783 @default.
• W3093446251 hasConcept C163716698 @default.
• W3093446251 hasConcept C178790620 @default.
• W3093446251 hasConcept C185592680 @default.
• W3093446251 hasConcept C192562407 @default.
• W3093446251 hasConcept C193016168 @default.
• W3093446251 hasConcept C202878990 @default.
• W3093446251 hasConcept C20702342 @default.
• W3093446251 hasConcept C2522874641 @default.
• W3093446251 hasConcept C2776800370 @default.
• W3093446251 hasConcept C2777822432 @default.
• W3093446251 hasConcept C2777993257 @default.
• W3093446251 hasConcept C2779343474 @default.
• W3093446251 hasConcept C41008148 @default.
• W3093446251 hasConcept C41895202 @default.
• W3093446251 hasConcept C75806775 @default.
• W3093446251 hasConcept C86803240 @default.
• W3093446251 hasConcept C93877712 @default.

• next