FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3093480265> ?p ?o ?g. }

• W3093480265 abstract "LRP1 is a large endocytic modular receptor that plays a crucial role in the scavenging of apoptotic material through binding to pattern-recognition molecules. It is a membrane anchored receptor of the LDL receptor family with 4 extracellular clusters of ligand binding modules called cysteine rich complement-type repeats that are involved in the interaction of LRP1 with its numerous ligands. Complement C1q was shown to interact with LRP1 and to be implicated in the phagocytosis of apoptotic cells. The present work aimed at exploring how these two large molecules interact at the molecular level using a dissection strategy. For that purpose, recombinant LRP1 clusters II, III and IV were produced in mammalian HEK293F cells and their binding properties were investigated. Clusters II and IV were found to interact specifically and efficiently with C1q with KDs in the nanomolar range. The use of truncated C1q fragments and recombinant mutated C1q allowed to localize more precisely the binding site for LRP1 on the collagen-like regions of C1q (CLRs), nearby the site that is implicated in the interaction with the cognate protease tetramer C1r2s2. This site could be a common anchorage for other ligands of C1q CLRs such as sulfated proteoglycans and Complement receptor type 1. The use of a cellular model, consisting in CHO LRP1-null cells transfected with full-length LRP1 or a cluster IV minireceptor (mini IV) confirmed that mini IV interacts with C1q at the cell membrane as well as full-length LRP1. Further cellular interaction studies finally highlighted that mini IV can endorse the full-length LRP1 binding efficiency for apoptotic cells and that C1q has no impact on this interaction." @default.
• W3093480265 created "2020-10-29" @default.
• W3093480265 creator A5025430502 @default.
• W3093480265 creator A5035748181 @default.
• W3093480265 creator A5039114113 @default.
• W3093480265 creator A5044598176 @default.
• W3093480265 creator A5046769409 @default.
• W3093480265 creator A5054976883 @default.
• W3093480265 creator A5056346620 @default.
• W3093480265 creator A5063144693 @default.
• W3093480265 creator A5071598397 @default.
• W3093480265 creator A5080246435 @default.
• W3093480265 creator A5000456924 @default.
• W3093480265 date "2020-10-21" @default.
• W3093480265 modified "2023-10-12" @default.
• W3093480265 title "Complement C1q Interacts With LRP1 Clusters II and IV Through a Site Close but Different From the Binding Site of Its C1r and C1s-Associated Proteases" @default.
• W3093480265 cites W1500280864 @default.
• W3093480265 cites W1502410387 @default.
• W3093480265 cites W1511615796 @default.
• W3093480265 cites W1521036527 @default.
• W3093480265 cites W1539895365 @default.
• W3093480265 cites W1560644011 @default.
• W3093480265 cites W1596468411 @default.
• W3093480265 cites W1790999934 @default.
• W3093480265 cites W1794696801 @default.
• W3093480265 cites W1826270762 @default.
• W3093480265 cites W1837942605 @default.
• W3093480265 cites W1923431801 @default.
• W3093480265 cites W1929879919 @default.
• W3093480265 cites W1983623773 @default.
• W3093480265 cites W1986670606 @default.
• W3093480265 cites W1995120469 @default.
• W3093480265 cites W1997963958 @default.
• W3093480265 cites W1998236766 @default.
• W3093480265 cites W1999357965 @default.
• W3093480265 cites W2028024668 @default.
• W3093480265 cites W2055879245 @default.
• W3093480265 cites W2072583622 @default.
• W3093480265 cites W2085539726 @default.
• W3093480265 cites W2086481114 @default.
• W3093480265 cites W2101147897 @default.
• W3093480265 cites W2103126392 @default.
• W3093480265 cites W2104573359 @default.
• W3093480265 cites W2106313529 @default.
• W3093480265 cites W2110876203 @default.
• W3093480265 cites W2123755856 @default.
• W3093480265 cites W2126261381 @default.
• W3093480265 cites W2127256767 @default.
• W3093480265 cites W2133067364 @default.
• W3093480265 cites W2136097616 @default.
• W3093480265 cites W2154521687 @default.
• W3093480265 cites W2157805702 @default.
• W3093480265 cites W2162667381 @default.
• W3093480265 cites W2172148793 @default.
• W3093480265 cites W2542724930 @default.
• W3093480265 cites W2563055933 @default.
• W3093480265 cites W2622909676 @default.
• W3093480265 cites W2791897116 @default.
• W3093480265 cites W2793196316 @default.
• W3093480265 cites W2922153329 @default.
• W3093480265 cites W2993150161 @default.
• W3093480265 doi "https://doi.org/10.3389/fimmu.2020.583754" @default.
• W3093480265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7609443" @default.
• W3093480265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33193398" @default.
• W3093480265 hasPublicationYear "2020" @default.
• W3093480265 type Work @default.
• W3093480265 sameAs 3093480265 @default.
• W3093480265 citedByCount "5" @default.
• W3093480265 countsByYear W30934802652021 @default.
• W3093480265 countsByYear W30934802652022 @default.
• W3093480265 countsByYear W30934802652023 @default.
• W3093480265 crossrefType "journal-article" @default.
• W3093480265 hasAuthorship W3093480265A5000456924 @default.
• W3093480265 hasAuthorship W3093480265A5025430502 @default.
• W3093480265 hasAuthorship W3093480265A5035748181 @default.
• W3093480265 hasAuthorship W3093480265A5039114113 @default.
• W3093480265 hasAuthorship W3093480265A5044598176 @default.
• W3093480265 hasAuthorship W3093480265A5046769409 @default.
• W3093480265 hasAuthorship W3093480265A5054976883 @default.
• W3093480265 hasAuthorship W3093480265A5056346620 @default.
• W3093480265 hasAuthorship W3093480265A5063144693 @default.
• W3093480265 hasAuthorship W3093480265A5071598397 @default.
• W3093480265 hasAuthorship W3093480265A5080246435 @default.
• W3093480265 hasBestOaLocation W30934802651 @default.
• W3093480265 hasConcept C1009742 @default.
• W3093480265 hasConcept C107824862 @default.
• W3093480265 hasConcept C111684460 @default.
• W3093480265 hasConcept C139647698 @default.
• W3093480265 hasConcept C159654299 @default.
• W3093480265 hasConcept C170493617 @default.
• W3093480265 hasConcept C175773311 @default.
• W3093480265 hasConcept C179223381 @default.
• W3093480265 hasConcept C181199279 @default.
• W3093480265 hasConcept C182220744 @default.
• W3093480265 hasConcept C185592680 @default.
• W3093480265 hasConcept C2778163477 @default.
• W3093480265 hasConcept C2780072125 @default.
• W3093480265 hasConcept C28005876 @default.
• W3093480265 hasConcept C43554185 @default.
• W3093480265 hasConcept C54355233 @default.

• next