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- W3093592015 abstract "Abstract Infections are most frequent at the extremes of life, especially among newborns, reflecting age-specific differences in immunity. Monocytes maintain tissue-homeostasis and defence-readiness by escaping circulation in the absence of inflammation to become tissue-resident antigen presenting cells in vivo. Despite equivalent circulating levels, neonates demonstrate lower presence of monocytes inside peripheral tissues as compared to adults. To study the ability of monocytes to undergo autonomous transendothelial extravasation under biologically accurate circumstances we engineered a three-dimensional human vascular-interstitial model including collagen, fibronectin, primary endothelial cells and autologous untreated plasma. This microphysiological tissue construct enabled age-specific autonomous extravasation of monocytes through a confluent human endothelium in the absence of exogenous chemokines and activation. Both CD16− and CD16+ newborn monocytes demonstrated lower adherence and extravasation as compared to adults. In contrast, pre-activated tissue constructs were colonized by newborn monocytes at the same frequency than adult monocytes, suggesting that neonatal monocytes are capable of colonizing inflamed tissues. The presence of autologous plasma neither improved newborn homeostatic extravasation nor shaped age-specific differences in endothelial cytokines that could account for this impairment. Newborn monocytes demonstrated significantly lower surface expression of CD31 and CD11b, and mechanistic experiments using blocking antibodies confirmed a functional role for CD31 and CD54 in neonatal homeostatic extravasation. Our data suggests that newborn monocytes are intrinsically impaired in extravasation through quiescent endothelia, a phenomenon that could contribute to the divergent immune responsiveness to vaccines and susceptibility to infection observed during early life." @default.
- W3093592015 created "2020-10-29" @default.
- W3093592015 creator A5022924664 @default.
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- W3093592015 date "2020-10-20" @default.
- W3093592015 modified "2023-10-18" @default.
- W3093592015 title "Neonatal monocytes demonstrate impaired homeostatic extravasation into a microphysiological human vascular model" @default.
- W3093592015 cites W1481676924 @default.
- W3093592015 cites W1499048493 @default.
- W3093592015 cites W1502095639 @default.
- W3093592015 cites W1552767137 @default.
- W3093592015 cites W1575618103 @default.
- W3093592015 cites W1593824314 @default.
- W3093592015 cites W1594446519 @default.
- W3093592015 cites W1740633437 @default.
- W3093592015 cites W1882575789 @default.
- W3093592015 cites W1964924890 @default.
- W3093592015 cites W1970802765 @default.
- W3093592015 cites W1972588882 @default.
- W3093592015 cites W1973668473 @default.
- W3093592015 cites W1974937503 @default.
- W3093592015 cites W1978418704 @default.
- W3093592015 cites W1983106692 @default.
- W3093592015 cites W1984404713 @default.
- W3093592015 cites W2002660664 @default.
- W3093592015 cites W2004602779 @default.
- W3093592015 cites W2006518558 @default.
- W3093592015 cites W2007087804 @default.
- W3093592015 cites W2009230499 @default.
- W3093592015 cites W2013877631 @default.
- W3093592015 cites W2015837671 @default.
- W3093592015 cites W2015954946 @default.
- W3093592015 cites W2018407019 @default.
- W3093592015 cites W2020069346 @default.
- W3093592015 cites W2023078780 @default.
- W3093592015 cites W2026125174 @default.
- W3093592015 cites W2026252047 @default.
- W3093592015 cites W2027669792 @default.
- W3093592015 cites W2029829140 @default.
- W3093592015 cites W2030029841 @default.
- W3093592015 cites W2042926593 @default.
- W3093592015 cites W2047903168 @default.
- W3093592015 cites W2050681882 @default.
- W3093592015 cites W2052251033 @default.
- W3093592015 cites W2053900312 @default.
- W3093592015 cites W2055483871 @default.
- W3093592015 cites W2055958955 @default.
- W3093592015 cites W2056416839 @default.
- W3093592015 cites W2066475087 @default.
- W3093592015 cites W2068966033 @default.
- W3093592015 cites W2076552939 @default.
- W3093592015 cites W2079822769 @default.
- W3093592015 cites W2081926054 @default.
- W3093592015 cites W2086467701 @default.
- W3093592015 cites W2086845034 @default.
- W3093592015 cites W2088024377 @default.
- W3093592015 cites W2099144412 @default.
- W3093592015 cites W2102316212 @default.
- W3093592015 cites W2115541182 @default.
- W3093592015 cites W2119550637 @default.
- W3093592015 cites W2123870729 @default.
- W3093592015 cites W2124331173 @default.
- W3093592015 cites W2129672275 @default.
- W3093592015 cites W2134667046 @default.
- W3093592015 cites W2135935649 @default.
- W3093592015 cites W2139541440 @default.
- W3093592015 cites W2142589355 @default.
- W3093592015 cites W2146666642 @default.
- W3093592015 cites W2153251153 @default.
- W3093592015 cites W2155444336 @default.
- W3093592015 cites W2159495039 @default.
- W3093592015 cites W2167681235 @default.
- W3093592015 cites W2209528326 @default.
- W3093592015 cites W2338948488 @default.
- W3093592015 cites W2342859294 @default.
- W3093592015 cites W2416687760 @default.
- W3093592015 cites W2521964578 @default.
- W3093592015 cites W2539071828 @default.
- W3093592015 cites W2584128045 @default.
- W3093592015 cites W2607819139 @default.
- W3093592015 cites W2626120438 @default.
- W3093592015 cites W2717080149 @default.
- W3093592015 cites W2901229523 @default.
- W3093592015 cites W2920950392 @default.
- W3093592015 cites W2922877403 @default.
- W3093592015 cites W2941620462 @default.
- W3093592015 cites W2966771790 @default.
- W3093592015 cites W2974414775 @default.
- W3093592015 cites W2990765878 @default.
- W3093592015 cites W2990862858 @default.
- W3093592015 cites W2997709843 @default.
- W3093592015 cites W2998434468 @default.
- W3093592015 cites W4205166572 @default.
- W3093592015 doi "https://doi.org/10.1038/s41598-020-74639-z" @default.