FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3093663764> ?p ?o ?g. }

• W3093663764 abstract "ABSTRACT Necroptosis is a lytic, inflammatory cell death pathway that is dysregulated in many human pathologies. The pathway is executed by a core machinery comprising the RIPK1 and RIPK3 kinases, which assemble into necrosomes in the cytoplasm, and the terminal effector pseudokinase, MLKL. RIPK3-mediated phosphorylation of MLKL induces oligomerization and translocation to the plasma membrane where MLKL accumulates as hotspots and perturbs the lipid bilayer to cause death. The precise choreography of events in the pathway, where they occur within cells, and pathway differences between species, are of immense interest. However, they have been poorly characterized due to a dearth of validated antibodies for microscopy studies. Here, we describe a toolbox of antibodies for immunofluorescent detection of the core necroptosis effectors, RIPK1, RIPK3 and MLKL, and their phosphorylated forms, in human and mouse cells. By comparing reactivity with endogenous proteins in wild-type cells and knockout controls in basal and necroptosis-inducing conditions, we characterise the specificity of frequently-used commercial and recently-developed antibodies for detection of necroptosis signaling events. Importantly, our findings demonstrate that not all frequently-used antibodies are suitable for monitoring necroptosis by immunofluorescence microscopy, and methanol-is preferable to paraformaldehyde-fixation for robust detection of specific RIPK1, RIPK3 and MLKL signals." @default.
• W3093663764 created "2020-10-29" @default.
• W3093663764 creator A5001610383 @default.
• W3093663764 creator A5013054442 @default.
• W3093663764 creator A5017657101 @default.
• W3093663764 creator A5028698788 @default.
• W3093663764 creator A5029275926 @default.
• W3093663764 creator A5046678035 @default.
• W3093663764 creator A5047676306 @default.
• W3093663764 creator A5049789656 @default.
• W3093663764 creator A5061463554 @default.
• W3093663764 creator A5066633511 @default.
• W3093663764 creator A5085446408 @default.
• W3093663764 creator A5086108219 @default.
• W3093663764 creator A5091262490 @default.
• W3093663764 date "2020-10-26" @default.
• W3093663764 modified "2023-10-17" @default.
• W3093663764 title "A toolbox for imaging RIPK1, RIPK3 and MLKL in mouse and human cells" @default.
• W3093663764 cites W1515879175 @default.
• W3093663764 cites W1537667127 @default.
• W3093663764 cites W1809156910 @default.
• W3093663764 cites W1859746580 @default.
• W3093663764 cites W1972410570 @default.
• W3093663764 cites W1973594803 @default.
• W3093663764 cites W1979032223 @default.
• W3093663764 cites W1983151602 @default.
• W3093663764 cites W1993613360 @default.
• W3093663764 cites W1998983498 @default.
• W3093663764 cites W2007303185 @default.
• W3093663764 cites W2026477080 @default.
• W3093663764 cites W2034006241 @default.
• W3093663764 cites W2041738154 @default.
• W3093663764 cites W2052605153 @default.
• W3093663764 cites W2052740597 @default.
• W3093663764 cites W2060204808 @default.
• W3093663764 cites W2069800916 @default.
• W3093663764 cites W2072179322 @default.
• W3093663764 cites W2078141613 @default.
• W3093663764 cites W2087232320 @default.
• W3093663764 cites W2091363184 @default.
• W3093663764 cites W2099540110 @default.
• W3093663764 cites W2109882045 @default.
• W3093663764 cites W2118621467 @default.
• W3093663764 cites W2120626244 @default.
• W3093663764 cites W2130776456 @default.
• W3093663764 cites W2132161879 @default.
• W3093663764 cites W2138887185 @default.
• W3093663764 cites W2146358940 @default.
• W3093663764 cites W2159190959 @default.
• W3093663764 cites W2178250841 @default.
• W3093663764 cites W2265728551 @default.
• W3093663764 cites W2290564862 @default.
• W3093663764 cites W2413627622 @default.
• W3093663764 cites W2464409316 @default.
• W3093663764 cites W2509943971 @default.
• W3093663764 cites W2547118804 @default.
• W3093663764 cites W2573286366 @default.
• W3093663764 cites W2575767307 @default.
• W3093663764 cites W2604229956 @default.
• W3093663764 cites W2610202481 @default.
• W3093663764 cites W2618633434 @default.
• W3093663764 cites W2761041020 @default.
• W3093663764 cites W2790556376 @default.
• W3093663764 cites W2792417102 @default.
• W3093663764 cites W2803164013 @default.
• W3093663764 cites W2903516075 @default.
• W3093663764 cites W2911249293 @default.
• W3093663764 cites W2921397389 @default.
• W3093663764 cites W2953983951 @default.
• W3093663764 cites W2976299346 @default.
• W3093663764 cites W2988241748 @default.
• W3093663764 cites W2991594838 @default.
• W3093663764 cites W3004946110 @default.
• W3093663764 cites W3007474161 @default.
• W3093663764 cites W3014714615 @default.
• W3093663764 cites W3035828086 @default.
• W3093663764 cites W3035955336 @default.
• W3093663764 cites W3035966325 @default.
• W3093663764 cites W3044264283 @default.
• W3093663764 cites W3049118667 @default.
• W3093663764 cites W3088096249 @default.
• W3093663764 cites W3089373202 @default.
• W3093663764 cites W4229664383 @default.
• W3093663764 doi "https://doi.org/10.1101/2020.10.26.356063" @default.
• W3093663764 hasPublicationYear "2020" @default.
• W3093663764 type Work @default.
• W3093663764 sameAs 3093663764 @default.
• W3093663764 citedByCount "3" @default.
• W3093663764 countsByYear W30936637642021 @default.
• W3093663764 countsByYear W30936637642022 @default.
• W3093663764 crossrefType "posted-content" @default.
• W3093663764 hasAuthorship W3093663764A5001610383 @default.
• W3093663764 hasAuthorship W3093663764A5013054442 @default.
• W3093663764 hasAuthorship W3093663764A5017657101 @default.
• W3093663764 hasAuthorship W3093663764A5028698788 @default.
• W3093663764 hasAuthorship W3093663764A5029275926 @default.
• W3093663764 hasAuthorship W3093663764A5046678035 @default.
• W3093663764 hasAuthorship W3093663764A5047676306 @default.
• W3093663764 hasAuthorship W3093663764A5049789656 @default.
• W3093663764 hasAuthorship W3093663764A5061463554 @default.

• next