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- W3093701411 abstract "Abstract Currently there is no in vitro diagnostic test for acute ischemic stroke (AIS), yet rapid diagnosis is crucial for effective thrombolytic treatment. We previously demonstrated the utility of CD8(+) T-cells’ mRNA expression for AIS detection; however extracellular vesicles (EVs) were not evaluated as a source of mRNA for AIS testing. We now report a microfluidic device for the rapid and efficient affinity-enrichment of CD8(+) EVs and subsequent EV’s mRNA analysis using droplet digital PCR (ddPCR). The microfluidic device contains a dense array of micropillars modified with anti-CD8α monoclonal antibodies that enriched 158 ± 10 nm sized EVs at 4.3 ± 2.1 × 10 9 particles/100 µL of plasma. Analysis of mRNA from CD8(+) EVs and their parental T-cells revealed correlation in the expression for AIS-specific genes in both cell lines and healthy donors. In a blinded study, 80% test positivity for AIS patients and controls was revealed with a total analysis time of 3.7 h." @default.
- W3093701411 created "2020-10-29" @default.
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- W3093701411 date "2020-10-26" @default.
- W3093701411 modified "2023-10-18" @default.
- W3093701411 title "Affinity enrichment of extracellular vesicles from plasma reveals mRNA changes associated with acute ischemic stroke" @default.
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- W3093701411 doi "https://doi.org/10.1038/s42003-020-01336-y" @default.
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