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- W3093729315 abstract "Abstract The utilization of one-carbon compounds, such as methanol, as a feedstock for production of high value compounds is a fast developing area within cell factory engineering. Methanol is a cheap and easily stored compound, which can be produced from CO2 and renewable energy. Engineering microbial cell factories for utilization of methanol could therefore contribute to decreasing the amounts of abundant CO2 and thereby contribute to a more sustainable world. In this project, the non-conventional oleaginous yeast Yarrowia lipolytica was engineered for utilization of methanol as a substrate. Methanol is a toxic compound which can be oxidized into the even more toxic formaldehyde. Therefore, prior to the introduction of heterologous genes, the methanol and formaldehyde toxicity was determined on Strain A. The cells were determined to tolerate methanol to a level up to X M and formaldehyde to a level of Y mM. From the cultivation data the optimal methanol concentration for cultivations in this project was determined to be Z%. The first step of assimilation of methanol into the core metabolism was to introduce heterologous alcohol dehydrogenases which would oxidase the methanol into formaldehyde. For this, three candidates were tested, Enzyme A, Enzyme B and Enzyme C. The evaluation of the strain containing these genes revealed that Enzyme B and Enzyme C were not functioning well in Y. lipolytica and they were therefore discarded in favour of Enzyme A. Next, Pathway A and Pathway B were integrated separately into the strain carrying the Enzyme A genes. The Pathway A expressing strain showcased the highest efficiency in formaldehyde assimilation as well as in methanol consumption. To sum up, we successfully engineered Y. lipolytica to assimilate methanol. Further evaluation and optimization will be needed on the strain to ensure that the carbon provided by the methanol is contributing to a production of high value compounds. (Less)" @default.
- W3093729315 created "2020-10-29" @default.
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- W3093729315 date "2020-01-01" @default.
- W3093729315 modified "2023-09-27" @default.
- W3093729315 title "Engineering Yarrowia lipolytica for methanol utilization" @default.
- W3093729315 hasPublicationYear "2020" @default.
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