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- W3093781720 abstract "Novel laser light sources in the mid-infrared region enable new spectroscopy schemes beyond classical absorption spectroscopy. Herein, we introduce a refractive index sensor based on a Mach-Zehnder interferometer and an external-cavity quantum cascade laser that allows rapid acquisition of high-resolution spectra of liquid-phase samples, sensitive to relative refractive index changes down to 10 −7 . Dispersion spectra of three model proteins in deuterated solution were recorded at concentrations as low as 0.25 mg mL −1 . Comparison with Kramers-Kronig-transformed Fourier transform infrared absorbance spectra revealed high conformance, and obtained figures of merit compare well with conventional high-end FTIR spectroscopy. Finally, we performed partial least squares-based multivariate analysis of a complex ternary protein mixture to showcase the potential of dispersion spectroscopy utilizing the developed sensor to tackle complex analytical problems. The results indicate that laser-based dispersion sensing can be successfully used for qualitative and quantitative analysis of proteins." @default.
- W3093781720 created "2020-10-29" @default.
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- W3093781720 date "2020-11-18" @default.
- W3093781720 modified "2023-10-17" @default.
- W3093781720 title "Mid-IR refractive index sensor for detecting proteins employing an external cavity quantum cascade laser-based Mach-Zehnder interferometer" @default.
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- W3093781720 doi "https://doi.org/10.1364/oe.403981" @default.
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